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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     869
NP_060250                                                              17    2528
XP_006716523                                                           17    2528
XP_006716522                                                           17    2528
XP_005251324                                                           17    2528
XP_005251323                                                           17    2528
NP_001116251                                                           16    7355
NP_079061                                                              16    7355
NP_150094                                                              15    9605
XP_006710739                                                           15    9605
XP_005270979                                                           15    9605
NP_056972                                                              15    9605

>NP_005219
          Length = 1210

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REATSSKAN 9
           REATS KAN
Sbjct: 748 REATSPKAN 756



>NP_060250
          Length = 2997

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    REATSSKAN 9
            REATSS +N
Sbjct: 2449 REATSSTSN 2457



>XP_006716523
          Length = 1912

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    REATSSKAN 9
            REATSS +N
Sbjct: 1364 REATSSTSN 1372



>XP_006716522
          Length = 2192

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    REATSSKAN 9
            REATSS +N
Sbjct: 1644 REATSSTSN 1652



>XP_005251324
          Length = 2996

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    REATSSKAN 9
            REATSS +N
Sbjct: 2448 REATSSTSN 2456



>XP_005251323
          Length = 2997

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    REATSSKAN 9
            REATSS +N
Sbjct: 2449 REATSSTSN 2457



>NP_001116251
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REATSSKAN 9
           REAT  K+N
Sbjct: 162 REATLQKSN 170



>NP_079061
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REATSSKAN 9
           REAT  K+N
Sbjct: 162 REATLQKSN 170



>NP_150094
          Length = 3564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ATSSKAN 9
            ATSSK N
Sbjct: 3380 ATSSKVN 3386



>XP_006710739
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ATSSKAN 9
           ATS KAN
Sbjct: 311 ATSDKAN 317



>XP_005270979
          Length = 728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ATSSKAN 9
           ATS KAN
Sbjct: 311 ATSDKAN 317



>NP_056972
          Length = 728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   ATSSKAN 9
           ATS KAN
Sbjct: 311 ATSDKAN 317



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.278   0.0893    0.195 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 219,041
Number of extensions: 201
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)