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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              19     869
NP_001087241                                                           19     869
NP_004110                                                              19     869
XP_005265799                                                           19     869
XP_005265798                                                           19     869
XP_005265797                                                           19     869
NP_005202                                                              18    1935
NP_001275634                                                           18    1935
NP_001035275                                                           17    4312
XP_006723530                                                           17    4312
XP_006723529                                                           17    4312
NP_996898                                                              15    9605
NP_001166126                                                           15    9605
NP_060730                                                              15    9605
NP_003921                                                              15    9605
NP_001139754                                                           15    9605
XP_005268081                                                           15    9605
XP_005249958                                                           15    9605
XP_005247321                                                           15    9605
XP_006713623                                                           15    9605
NP_003127                                                              15    9605

>NP_000213
          Length = 976

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           REALMS+LK
Sbjct: 634 REALMSELK 642



>NP_001087241
          Length = 972

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           REALMS+LK
Sbjct: 630 REALMSELK 638



>NP_004110
          Length = 993

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           REALMS+LK
Sbjct: 655 REALMSELK 663



>XP_005265799
          Length = 973

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           REALMS+LK
Sbjct: 631 REALMSELK 639



>XP_005265798
          Length = 976

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           REALMS+LK
Sbjct: 635 REALMSELK 643



>XP_005265797
          Length = 977

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           REALMS+LK
Sbjct: 635 REALMSELK 643



>NP_005202
          Length = 972

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           +EALMS+LK
Sbjct: 627 KEALMSELK 635



>NP_001275634
          Length = 972

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   REALMSKLK 9
           +EALMS+LK
Sbjct: 627 KEALMSELK 635



>NP_001035275
          Length = 523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EALMSKLK 9
           EALM+K+K
Sbjct: 110 EALMTKIK 117



>XP_006723530
          Length = 470

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  EALMSKLK 9
          EALM+K+K
Sbjct: 57 EALMTKIK 64



>XP_006723529
          Length = 523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   EALMSKLK 9
           EALM+K+K
Sbjct: 110 EALMTKIK 117



>NP_996898
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALMSKLK 9
           ALMSK+K
Sbjct: 476 ALMSKVK 482



>NP_001166126
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSKLK 9
           EALM+K K
Sbjct: 176 EALMTKTK 183



>NP_060730
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSKLK 9
           EALM+K K
Sbjct: 110 EALMTKTK 117



>NP_003921
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REALMSKLK 9
          RE+L+ K+K
Sbjct: 45 RESLIKKIK 53



>NP_001139754
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSKLK 9
           EAL+ KLK
Sbjct: 267 EALIEKLK 274



>XP_005268081
          Length = 447

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSKLK 9
           EAL+ KLK
Sbjct: 316 EALIEKLK 323



>XP_005249958
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  REALMSKLK 9
          RE+L+ K+K
Sbjct: 45 RESLIKKIK 53



>XP_005247321
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALMSKLK 9
           ALMSK+K
Sbjct: 476 ALMSKVK 482



>XP_006713623
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALMSKLK 9
           ALMSK+K
Sbjct: 459 ALMSKVK 465



>NP_003127
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   EALMSKLK 9
           EAL+ KLK
Sbjct: 316 EALIEKLK 323



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.123    0.281 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 267,037
Number of extensions: 412
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)