Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001185761                                                           18    1482
NP_001185763                                                           18    1482
NP_055459                                                              18    1482
XP_006726412                                                           18    1482
XP_006726410                                                           18    1482
XP_006711760                                                           18    1482
XP_006711719                                                           18    1482
XP_006711718                                                           18    1482
XP_006711717                                                           18    1482
XP_006711716                                                           18    1482
XP_006711715                                                           18    1482
XP_006711714                                                           18    1482
XP_006711713                                                           18    1482
XP_006711712                                                           18    1482
XP_006711711                                                           18    1482
XP_006711710                                                           18    1482
XP_006711709                                                           18    1482
XP_005277503                                                           18    1482
XP_005273038                                                           18    1482
XP_006711708                                                           18    1482
XP_005277500                                                           18    1482
XP_006711707                                                           18    1482
XP_005277499                                                           18    1482
XP_006711706                                                           18    1482
XP_006711705                                                           18    1482
XP_006711164                                                           18    1482
XP_005275915                                                           18    1482
XP_006710140                                                           18    1482
NP_001189358                                                           17    2528
NP_057234                                                              17    2528
XP_006725228                                                           17    2528
XP_005267815                                                           17    2528
NP_001185771                                                           16    5631
NP_006422                                                              16    5631
NP_001008395                                                           16    7355
NP_001243481                                                           15    9605
NP_065100                                                              15    9605
NP_078884                                                              15    9605
NP_001273450                                                           15    9605
NP_001273451                                                           15    9605
NP_001096068                                                           15    9605
NP_001096067                                                           15    9605
NP_001096069                                                           15    9605
NP_001096070                                                           15    9605
XP_006720745                                                           15    9605
XP_005254716                                                           15    9605
XP_006720744                                                           15    9605
XP_006720743                                                           15    9605
XP_006720742                                                           15    9605
XP_006720740                                                           15    9605
XP_005253771                                                           15    9605
XP_006719050                                                           15    9605
XP_005253767                                                           15    9605
XP_005253768                                                           15    9605
XP_005263095                                                           15    9605
XP_005263096                                                           15    9605
XP_005263093                                                           15    9605
XP_005263094                                                           15    9605
NP_006165                                                              15    9605
>NP_001185761
          Length = 2240

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1827 REALLSSR 1834
>NP_001185763
          Length = 2362

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1933 REALLSSR 1940
>NP_055459
          Length = 2346

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1933 REALLSSR 1940
>XP_006726412
          Length = 140

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  REALLSSR 8
          REALLSSR
Sbjct: 5  REALLSSR 12
>XP_006726410
          Length = 2087

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1674 REALLSSR 1681
>XP_006711760
          Length = 140

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  REALLSSR 8
          REALLSSR
Sbjct: 5  REALLSSR 12
>XP_006711719
          Length = 2302

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1889 REALLSSR 1896
>XP_006711718
          Length = 1436

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   REALLSSR 8
           REALLSSR
Sbjct: 940 REALLSSR 947
>XP_006711717
          Length = 2385

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1889 REALLSSR 1896
>XP_006711716
          Length = 2411

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1998 REALLSSR 2005
>XP_006711715
          Length = 2428

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1932 REALLSSR 1939
>XP_006711714
          Length = 2429

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1933 REALLSSR 1940
>XP_006711713
          Length = 2432

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1936 REALLSSR 1943
>XP_006711712
          Length = 2473

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_006711711
          Length = 2482

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1986 REALLSSR 1993
>XP_006711710
          Length = 2494

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1998 REALLSSR 2005
>XP_006711709
          Length = 2495

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1999 REALLSSR 2006
>XP_005277503
          Length = 2498

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_005273038
          Length = 2509

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_006711708
          Length = 2512

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_005277500
          Length = 2525

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_006711707
          Length = 2553

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_005277499
          Length = 2561

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_006711706
          Length = 2591

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2095 REALLSSR 2102
>XP_006711705
          Length = 2592

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 2096 REALLSSR 2103
>XP_006711164
          Length = 2087

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    REALLSSR 8
            REALLSSR
Sbjct: 1674 REALLSSR 1681
>XP_005275915
          Length = 109

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  REALLSSR 8
          REALLSSR
Sbjct: 5  REALLSSR 12
>XP_006710140
          Length = 290

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   REALLSSR 8
           REALLSSR
Sbjct: 186 REALLSSR 193
>NP_001189358
          Length = 635

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EALLSSRF 9
           EALLS+RF
Sbjct: 386 EALLSARF 393
>NP_057234
          Length = 587

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EALLSSRF 9
           EALLS+RF
Sbjct: 338 EALLSARF 345
>XP_006725228
          Length = 635

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EALLSSRF 9
           EALLS+RF
Sbjct: 386 EALLSARF 393
>XP_005267815
          Length = 635

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   EALLSSRF 9
           EALLS+RF
Sbjct: 386 EALLSARF 393
>NP_001185771
          Length = 488

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1  REALLSS 7
          REALLSS
Sbjct: 91 REALLSS 97
>NP_006422
          Length = 535

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   REALLSS 7
           REALLSS
Sbjct: 138 REALLSS 144
>NP_001008395
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  REALLSSRF 9
          REA L +RF
Sbjct: 84 REAALDARF 92
>NP_001243481
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 249 EALLDQRF 256
>NP_065100
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 239 RDAAYSSRF 247
>NP_078884
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 163 EALLDQRF 170
>NP_001273450
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 158 RDAAYSSRF 166
>NP_001273451
          Length = 257

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 158 RDAAYSSRF 166
>NP_001096068
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REALLSSR 8
           R+ALLS R
Sbjct: 141 RDALLSQR 148
>NP_001096067
          Length = 596

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REALLSSR 8
           R+ALLS R
Sbjct: 141 RDALLSQR 148
>NP_001096069
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REALLSSR 8
           R+ALLS R
Sbjct: 141 RDALLSQR 148
>NP_001096070
          Length = 584

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   REALLSSR 8
           R+ALLS R
Sbjct: 141 RDALLSQR 148
>XP_006720745
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 163 EALLDQRF 170
>XP_005254716
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 163 EALLDQRF 170
>XP_006720744
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 163 EALLDQRF 170
>XP_006720743
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 163 EALLDQRF 170
>XP_006720742
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 206 EALLDQRF 213
>XP_006720740
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EALLSSRF 9
           EALL  RF
Sbjct: 240 EALLDQRF 247
>XP_005253771
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 140 RDAAYSSRF 148
>XP_006719050
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 140 RDAAYSSRF 148
>XP_005253767
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 232 RDAAYSSRF 240
>XP_005253768
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REALLSSRF 9
           R+A  SSRF
Sbjct: 232 RDAAYSSRF 240
>XP_005263095
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALLSSRF 9
           ALLSSR+
Sbjct: 190 ALLSSRY 196
>XP_005263096
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALLSSRF 9
           ALLSSR+
Sbjct: 190 ALLSSRY 196
>XP_005263093
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALLSSRF 9
           ALLSSR+
Sbjct: 190 ALLSSRY 196
>XP_005263094
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALLSSRF 9
           ALLSSR+
Sbjct: 190 ALLSSRY 196
>NP_006165
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ALLSSRF 9
           ALLSSR+
Sbjct: 190 ALLSSRY 196
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.134    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 297,039
Number of extensions: 353
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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