Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              20     509
NP_004220                                                              17    4312
XP_005272758                                                           17    4312
XP_005272757                                                           17    4312
XP_005272756                                                           17    4312
NP_001157745                                                           16    5631
NP_001074010                                                           16    5631
XP_005254311                                                           16    5631
XP_006720508                                                           16    5631
XP_006720507                                                           16    5631
XP_005254310                                                           16    5631
XP_005254309                                                           16    5631
XP_005254306                                                           16    5631
XP_005254305                                                           16    5631
XP_005254304                                                           16    5631
XP_005254303                                                           16    5631
XP_006720506                                                           16    5631
XP_005254302                                                           16    5631
XP_005254301                                                           16    5631
XP_006720505                                                           16    5631
XP_005254300                                                           16    5631
NP_001035904                                                           16    7355
NP_596870                                                              16    7355
NP_848561                                                              16    7355
NP_001243353                                                           16    7355
NP_000846                                                              16    7355
NP_001230469                                                           15    9605
NP_062548                                                              15    9605
NP_057239                                                              15    9605
NP_004663                                                              15    9605
NP_001254                                                              15    9605
NP_001230470                                                           15    9605
XP_006726201                                                           15    9605
XP_005256979                                                           15    9605
XP_006721694                                                           15    9605
XP_005262744                                                           15    9605
XP_005246086                                                           15    9605
XP_005245779                                                           15    9605
XP_005245778                                                           15    9605
XP_005245777                                                           15    9605
XP_005245776                                                           15    9605
XP_005245775                                                           15    9605
XP_005245774                                                           15    9605
NP_005830                                                              15    9605
>NP_005219
          Length = 1210

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   REAISPKAN 9
           REA SPKAN
Sbjct: 748 REATSPKAN 756
>NP_004220
          Length = 1454

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    REAISPKAN 9
            R A+SPK N
Sbjct: 1250 RVALSPKTN 1258
>XP_005272758
          Length = 1338

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    REAISPKAN 9
            R A+SPK N
Sbjct: 1134 RVALSPKTN 1142
>XP_005272757
          Length = 1410

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    REAISPKAN 9
            R A+SPK N
Sbjct: 1206 RVALSPKTN 1214
>XP_005272756
          Length = 1443

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    REAISPKAN 9
            R A+SPK N
Sbjct: 1250 RVALSPKTN 1258
>NP_001157745
          Length = 3065

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1999 EAVDPEAN 2006
>NP_001074010
          Length = 2856

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1790 EAVDPEAN 1797
>XP_005254311
          Length = 1627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EAISPKAN 9
           EA+ P+AN
Sbjct: 560 EAVDPEAN 567
>XP_006720508
          Length = 2658

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1591 EAVDPEAN 1598
>XP_006720507
          Length = 2763

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1696 EAVDPEAN 1703
>XP_005254310
          Length = 2906

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1839 EAVDPEAN 1846
>XP_005254309
          Length = 2956

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1889 EAVDPEAN 1896
>XP_005254306
          Length = 3066

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 1999 EAVDPEAN 2006
>XP_005254305
          Length = 3114

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254304
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254303
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_006720506
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254302
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254301
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_006720505
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>XP_005254300
          Length = 3115

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    EAISPKAN 9
            EA+ P+AN
Sbjct: 2048 EAVDPEAN 2055
>NP_001035904
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EAISPKAN 9
           EAI PK N
Sbjct: 143 EAIPPKMN 150
>NP_596870
          Length = 5604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    EAISPKAN 9
            EAI PK N
Sbjct: 5172 EAIEPKDN 5179
>NP_848561
          Length = 254

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   EAISPKAN 9
           EAI PK N
Sbjct: 179 EAIPPKMN 186
>NP_001243353
          Length = 763

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EAISPKAN 9
           E +SPK N
Sbjct: 365 EIVSPKVN 372
>NP_000846
          Length = 732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   EAISPKAN 9
           E +SPK N
Sbjct: 365 EIVSPKVN 372
>NP_001230469
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   REAISP 6
           REA+SP
Sbjct: 320 REAVSP 325
>NP_062548
          Length = 455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   ISPKAN 9
           +SPKAN
Sbjct: 269 LSPKAN 274
>NP_057239
          Length = 603

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   REAISPKA 8
           RE++SP A
Sbjct: 297 RESVSPTA 304
>NP_004663
          Length = 1288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    REAISPKA 8
            +EA+SP++
Sbjct: 1125 KEAVSPRS 1132
>NP_001254
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  REAISP 6
          REA+SP
Sbjct: 14 REAVSP 19
>NP_001230470
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   REAISP 6
           REA+SP
Sbjct: 262 REAVSP 267
>XP_006726201
          Length = 245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  ISPKAN 9
          +SPKAN
Sbjct: 59 LSPKAN 64
>XP_005256979
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   REAISP 6
           REA+SP
Sbjct: 262 REAVSP 267
>XP_006721694
          Length = 621

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   REAISP 6
           REA+SP
Sbjct: 320 REAVSP 325
>XP_005262744
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  REAISP 6
          REA+SP
Sbjct: 14 REAVSP 19
>XP_005246086
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    REAISPKA 8
            +EA+SP++
Sbjct: 1117 KEAVSPRS 1124
>XP_005245779
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 655 REARSPQPN 663
>XP_005245778
          Length = 902

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 669 REARSPQPN 677
>XP_005245777
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 682 REARSPQPN 690
>XP_005245776
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 683 REARSPQPN 691
>XP_005245775
          Length = 917

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 684 REARSPQPN 692
>XP_005245774
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 685 REARSPQPN 693
>NP_005830
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   REAISPKAN 9
           REA SP+ N
Sbjct: 671 REARSPQPN 679
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.115    0.297 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 689,116
Number of extensions: 788
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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