Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              20     390
XP_006711527                                                           16    5631
XP_005245411                                                           16    5631
NP_056142                                                              16    7355
NP_002438                                                              16    7355
NP_001231866                                                           16    7355
NP_001272369                                                           16    7355
NP_689969                                                              16    7355
NP_001272368                                                           16    7355
NP_001229743                                                           16    7355
NP_001229742                                                           16    7355
NP_055757                                                              16    7355
NP_001229740                                                           16    7355
NP_001229738                                                           16    7355
XP_006715445                                                           16    7355
XP_005248741                                                           16    7355
XP_005248740                                                           16    7355
XP_006715444                                                           16    7355
XP_005248739                                                           16    7355
XP_005248738                                                           16    7355
XP_005248737                                                           16    7355
XP_005248736                                                           16    7355
XP_005248335                                                           16    7355
XP_006714526                                                           16    7355
XP_005265227                                                           16    7355
XP_006711311                                                           16    7355
XP_005245106                                                           16    7355
NP_001153777                                                           15    9605
NP_079136                                                              15    9605
NP_859074                                                              15    9605
NP_659473                                                              15    9605
NP_004064                                                              15    9605
NP_001186094                                                           15    9605
XP_005257183                                                           15    9605
XP_005257182                                                           15    9605
XP_006721805                                                           15    9605
XP_006721804                                                           15    9605
XP_005257179                                                           15    9605
XP_006721803                                                           15    9605
XP_005257176                                                           15    9605
XP_006721334                                                           15    9605
XP_005273535                                                           15    9605
XP_005273534                                                           15    9605
NP_849150                                                              15    9605
NP_001032402                                                           15    9605
>NP_004251
          Length = 1208

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RDTDQALLS 9
           RDTDQALL+
Sbjct: 682 RDTDQALLT 690
>XP_006711527
          Length = 333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RDTDQALL 8
           RD+D ALL
Sbjct: 210 RDSDDALL 217
>XP_005245411
          Length = 380

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RDTDQALL 8
           RD+D ALL
Sbjct: 257 RDSDDALL 264
>NP_056142
          Length = 1016

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DTDQALLS 9
           DTD+ LLS
Sbjct: 750 DTDRPLLS 757
>NP_002438
          Length = 1400

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RDTDQALLS 9
            RD D ALL+
Sbjct: 1060 RDLDSALLA 1068
>NP_001231866
          Length = 1351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RDTDQALLS 9
            RD D ALL+
Sbjct: 1011 RDLDSALLA 1019
>NP_001272369
          Length = 1385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RDTDQALL 8
           +DT++ALL
Sbjct: 237 KDTEEALL 244
>NP_689969
          Length = 1708

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RDTDQALL 8
           +DT++ALL
Sbjct: 522 KDTEEALL 529
>NP_001272368
          Length = 1732

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RDTDQALL 8
           +DT++ALL
Sbjct: 522 KDTEEALL 529
>NP_001229743
          Length = 663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 595 RDTSQAL 601
>NP_001229742
          Length = 692

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 624 RDTSQAL 630
>NP_055757
          Length = 722

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 654 RDTSQAL 660
>NP_001229740
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 659 RDTSQAL 665
>NP_001229738
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 659 RDTSQAL 665
>XP_006715445
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 628 RDTSQAL 634
>XP_005248741
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 524 RDTSQAL 530
>XP_005248740
          Length = 686

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 618 RDTSQAL 624
>XP_006715444
          Length = 721

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 653 RDTSQAL 659
>XP_005248739
          Length = 726

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 658 RDTSQAL 664
>XP_005248738
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 659 RDTSQAL 665
>XP_005248737
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 679 RDTSQAL 685
>XP_005248736
          Length = 752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RDT QAL
Sbjct: 684 RDTSQAL 690
>XP_005248335
          Length = 1423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RDTDQALL 8
           +DT++ALL
Sbjct: 237 KDTEEALL 244
>XP_006714526
          Length = 1670

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   RDTDQALL 8
           +DT++ALL
Sbjct: 522 KDTEEALL 529
>XP_005265227
          Length = 1401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    RDTDQALLS 9
            RD D ALL+
Sbjct: 1061 RDLDSALLA 1069
>XP_006711311
          Length = 950

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DTDQALLS 9
           DTD+ LLS
Sbjct: 684 DTDRPLLS 691
>XP_005245106
          Length = 970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DTDQALLS 9
           DTD+ LLS
Sbjct: 704 DTDRPLLS 711
>NP_001153777
          Length = 473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TDQALLS 9
           TDQ LLS
Sbjct: 433 TDQGLLS 439
>NP_079136
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TDQALLS 9
           TDQ LLS
Sbjct: 409 TDQGLLS 415
>NP_859074
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RDTDQALLS 9
           R+T QAL S
Sbjct: 316 RNTSQALFS 324
>NP_659473
          Length = 955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 862 RDNDQAI 868
>NP_004064
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   TDQALL 8
           TDQALL
Sbjct: 569 TDQALL 574
>NP_001186094
          Length = 955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 862 RDNDQAI 868
>XP_005257183
          Length = 547

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 454 RDNDQAI 460
>XP_005257182
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 555 RDNDQAI 561
>XP_006721805
          Length = 874

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 863 RDNDQAI 869
>XP_006721804
          Length = 908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 815 RDNDQAI 821
>XP_005257179
          Length = 913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 820 RDNDQAI 826
>XP_006721803
          Length = 956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 863 RDNDQAI 869
>XP_005257176
          Length = 956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 863 RDNDQAI 869
>XP_006721334
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   TDQALLS 9
           TDQ LLS
Sbjct: 436 TDQGLLS 442
>XP_005273535
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RDTDQALLS 9
           R+T QAL S
Sbjct: 140 RNTSQALFS 148
>XP_005273534
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   RDTDQALLS 9
           R+T QAL S
Sbjct: 303 RNTSQALFS 311
>NP_849150
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   DTDQALL 8
           DT QALL
Sbjct: 670 DTQQALL 676
>NP_001032402
          Length = 211

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDTDQAL 7
           RD DQA+
Sbjct: 118 RDNDQAI 124
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.125    0.314 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 497,755
Number of extensions: 760
Number of successful extensions: 45
Number of sequences better than 10000.0: 45
Number of HSP's gapped: 45
Number of HSP's successfully gapped: 45
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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