Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     869
NP_001263689                                                           19     869
NP_001119584                                                           19     869
NP_001119586                                                           19     869
NP_001263625                                                           19     869
NP_001119590                                                           19     869
NP_001263690                                                           19     869
NP_001119585                                                           19     869
NP_001263624                                                           19     869
NP_001119588                                                           19     869
NP_001119587                                                           19     869
NP_001119589                                                           19     869
NP_001263627                                                           18    1935
NP_001263628                                                           18    1935
NP_001263626                                                           18    1935
NP_001230133                                                           17    3301
NP_001135936                                                           17    3301
NP_057665                                                              17    3301
NP_064506                                                              17    4312
NP_055483                                                              15    9605
NP_038464                                                              15    9605
NP_995314                                                              15    9605
NP_006815                                                              15    9605
NP_001153408                                                           15    9605
NP_001626                                                              15    9605
NP_647477                                                              15    9605
XP_006715753                                                           15    9605
XP_006715752                                                           15    9605
XP_006712264                                                           15    9605
XP_006712263                                                           15    9605
XP_005246254                                                           15    9605
XP_005246253                                                           15    9605
XP_005246252                                                           15    9605
XP_006711970                                                           15    9605
XP_006711969                                                           15    9605
XP_005246249                                                           15    9605
XP_006711968                                                           15    9605
XP_006711967                                                           15    9605
>NP_000537
          Length = 393

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001263689
          Length = 354

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119584
          Length = 393

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001119586
          Length = 341

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001263625
          Length = 302

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119590
          Length = 354

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001263690
          Length = 354

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119585
          Length = 346

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 158 RAMAIYKQS 166
>NP_001263624
          Length = 307

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R MAIYKQS
Sbjct: 119 RAMAIYKQS 127
>NP_001119588
          Length = 209

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RDMAIYKQS 9
          R MAIYKQS
Sbjct: 26 RAMAIYKQS 34
>NP_001119587
          Length = 261

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RDMAIYKQS 9
          R MAIYKQS
Sbjct: 26 RAMAIYKQS 34
>NP_001119589
          Length = 214

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  RDMAIYKQS 9
          R MAIYKQS
Sbjct: 26 RAMAIYKQS 34
>NP_001263627
          Length = 182

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7
>NP_001263628
          Length = 187

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7
>NP_001263626
          Length = 234

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3 MAIYKQS 9
         MAIYKQS
Sbjct: 1 MAIYKQS 7
>NP_001230133
          Length = 296

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RDMAIYKQ 8
           RD+A+Y Q
Sbjct: 124 RDLAVYNQ 131
>NP_001135936
          Length = 296

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RDMAIYKQ 8
           RD+A+Y Q
Sbjct: 124 RDLAVYNQ 131
>NP_057665
          Length = 431

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   RDMAIYKQ 8
           RD+A+Y Q
Sbjct: 124 RDLAVYNQ 131
>NP_064506
          Length = 1516

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  RDMAIYKQS 9
          +++AIYKQ+
Sbjct: 72 QELAIYKQT 80
>NP_055483
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1580 EMAIYKK 1586
>NP_038464
          Length = 1128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1118 AVYKQS 1123
>NP_995314
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1124 AVYKQS 1129
>NP_006815
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R+M+ Y+Q+
Sbjct: 104 REMSFYRQA 112
>NP_001153408
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   RDMAIYKQS 9
           R+M+ Y+Q+
Sbjct: 159 REMSFYRQA 167
>NP_001626
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDMAIYK 7
           RD+A YK
Sbjct: 677 RDLATYK 683
>NP_647477
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDMAIYK 7
           RD+A YK
Sbjct: 635 RDLATYK 641
>XP_006715753
          Length = 819

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RDMAIYK 7
           RD+A YK
Sbjct: 801 RDLATYK 807
>XP_006715752
          Length = 1225

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    RDMAIYK 7
            RD+A YK
Sbjct: 1207 RDLATYK 1213
>XP_006712264
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1116 AVYKQS 1121
>XP_006712263
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    AIYKQS 9
            A+YKQS
Sbjct: 1122 AVYKQS 1127
>XP_005246254
          Length = 947

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   DMAIYKQ 8
           +MAIYK+
Sbjct: 578 EMAIYKK 584
>XP_005246253
          Length = 1038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   DMAIYKQ 8
           +MAIYK+
Sbjct: 669 EMAIYKK 675
>XP_005246252
          Length = 1850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1481 EMAIYKK 1487
>XP_006711970
          Length = 1948

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1579 EMAIYKK 1585
>XP_006711969
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1580 EMAIYKK 1586
>XP_005246249
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1580 EMAIYKK 1586
>XP_006711968
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1580 EMAIYKK 1586
>XP_006711967
          Length = 1949

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    DMAIYKQ 8
            +MAIYK+
Sbjct: 1580 EMAIYKK 1586
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.127    0.335 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 394,968
Number of extensions: 671
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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