Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003310                                                              19     869
NP_597676                                                              19     869
NP_001243779                                                           19     869
NP_596869                                                              19     869
XP_005246888                                                           19     869
XP_006712788                                                           19     869
XP_006712787                                                           19     869
XP_006712786                                                           19     869
NP_001254479                                                           19     869
NP_597681                                                              19     869
NP_004963                                                              18    1482
NP_733841                                                              18    1482
NP_004671                                                              18    1482
NP_001003895                                                           18    1482
NP_004816                                                              18    1482
XP_005251512                                                           18    1482
NP_001003894                                                           18    1482
NP_001001722                                                           18    1482
NP_001243489                                                           17    4312
NP_004935                                                              17    4312
NP_031392                                                              16    7355
NP_001007268                                                           16    7355
NP_001182570                                                           16    7355
NP_001167560                                                           16    7355
NP_114423                                                              16    7355
NP_001167561                                                           16    7355
XP_005260914                                                           16    7355
XP_005260913                                                           16    7355
XP_005246450                                                           16    7355
XP_005246449                                                           16    7355
NP_001120674                                                           15    9605
NP_001245142                                                           15    9605
NP_001120675                                                           15    9605
NP_060856                                                              15    9605
NP_001245141                                                           15    9605
NP_003015                                                              15    9605
NP_006018                                                              15    9605
NP_569082                                                              15    9605
NP_003677                                                              15    9605
XP_005261083                                                           15    9605
XP_006720039                                                           15    9605
XP_005268381                                                           15    9605
XP_005270035                                                           15    9605
XP_005263618                                                           15    9605
XP_005263614                                                           15    9605
XP_005263613                                                           15    9605
XP_005263612                                                           15    9605
XP_005263609                                                           15    9605
XP_005263607                                                           15    9605
XP_005273407                                                           15    9605
XP_006711903                                                           15    9605
NP_942090                                                              15    9605
>NP_003310
          Length = 26926

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RCN+KL+ E
Sbjct: 6843 RCNMKLVPE 6851
>NP_597676
          Length = 27051

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RCN+KL+ E
Sbjct: 6968 RCNMKLVPE 6976
>NP_001243779
          Length = 34350

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     RCNLKLIME 9
             RCN+KL+ E
Sbjct: 14267 RCNMKLVPE 14275
>NP_596869
          Length = 33423

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     RCNLKLIME 9
             RCN+KL+ E
Sbjct: 13340 RCNMKLVPE 13348
>XP_005246888
          Length = 22663

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RCN+KL+ E
Sbjct: 2580 RCNMKLVPE 2588
>XP_006712788
          Length = 24022

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RCN+KL+ E
Sbjct: 3939 RCNMKLVPE 3947
>XP_006712787
          Length = 33978

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     RCNLKLIME 9
             RCN+KL+ E
Sbjct: 13895 RCNMKLVPE 13903
>XP_006712786
          Length = 35484

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     RCNLKLIME 9
             RCN+KL+ E
Sbjct: 15401 RCNMKLVPE 15409
>NP_001254479
          Length = 35991

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1     RCNLKLIME 9
             RCN+KL+ E
Sbjct: 15908 RCNMKLVPE 15916
>NP_597681
          Length = 27118

 Score = 18.9 bits (37), Expect =   869
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RCN+KL+ E
Sbjct: 7035 RCNMKLVPE 7043
>NP_004963
          Length = 1132

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           R NLKLIME
Sbjct: 922 RRNLKLIME 930
>NP_733841
          Length = 540

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           RCN+KL +E
Sbjct: 498 RCNIKLELE 506
>NP_004671
          Length = 554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           RCN+KL +E
Sbjct: 498 RCNIKLELE 506
>NP_001003895
          Length = 554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           RCN+KL +E
Sbjct: 498 RCNIKLELE 506
>NP_004816
          Length = 541

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           RCN+KL +E
Sbjct: 499 RCNIKLELE 507
>XP_005251512
          Length = 1132

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           R NLKLIME
Sbjct: 922 RRNLKLIME 930
>NP_001003894
          Length = 540

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           RCN+KL +E
Sbjct: 498 RCNIKLELE 506
>NP_001001722
          Length = 541

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RCNLKLIME 9
           RCN+KL +E
Sbjct: 499 RCNIKLELE 507
>NP_001243489
          Length = 275

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RCNLKLIME 9
          RC++ L+ME
Sbjct: 51 RCDIILVME 59
>NP_004935
          Length = 305

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  RCNLKLIME 9
          RC++ L+ME
Sbjct: 51 RCDIILVME 59
>NP_031392
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RCNLKLI 7
           RCN K+I
Sbjct: 116 RCNRKMI 122
>NP_001007268
          Length = 1324

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RCNLKLI 7
           RCN K+I
Sbjct: 116 RCNRKMI 122
>NP_001182570
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RCNLKLI 7
           RCN K+I
Sbjct: 116 RCNRKMI 122
>NP_001167560
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CNLKLIM 8
           CNL L+M
Sbjct: 154 CNLDLLM 160
>NP_114423
          Length = 891

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CNLKLIM 8
           CNL L+M
Sbjct: 170 CNLDLLM 176
>NP_001167561
          Length = 918

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CNLKLIM 8
           CNL L+M
Sbjct: 197 CNLDLLM 203
>XP_005260914
          Length = 856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CNLKLIM 8
           CNL L+M
Sbjct: 135 CNLDLLM 141
>XP_005260913
          Length = 998

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   CNLKLIM 8
           CNL L+M
Sbjct: 315 CNLDLLM 321
>XP_005246450
          Length = 1461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RCNLKLI 7
           RCN K+I
Sbjct: 116 RCNRKMI 122
>XP_005246449
          Length = 1463

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   RCNLKLI 7
           RCN K+I
Sbjct: 116 RCNRKMI 122
>NP_001120674
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 307 RCGIFLILE 315
>NP_001245142
          Length = 397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 305 RCGIFLILE 313
>NP_001120675
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 307 RCGIFLILE 315
>NP_060856
          Length = 399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 307 RCGIFLILE 315
>NP_001245141
          Length = 453

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 361 RCGIFLILE 369
>NP_003015
          Length = 1721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2    CNLKLI 7
            CN+KL+
Sbjct: 1291 CNIKLL 1296
>NP_006018
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CNLKLI 7
           CN+KL+
Sbjct: 707 CNIKLL 712
>NP_569082
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CNLKLI 7
           CN+KL+
Sbjct: 707 CNIKLL 712
>NP_003677
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CNLKLI 7
           CN+KL+
Sbjct: 707 CNIKLL 712
>XP_005261083
          Length = 1716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2    CNLKLI 7
            CN+KL+
Sbjct: 1286 CNIKLL 1291
>XP_006720039
          Length = 232

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 140 RCGIFLILE 148
>XP_005268381
          Length = 397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RCNLKLIME 9
           RC + LI+E
Sbjct: 305 RCGIFLILE 313
>XP_005270035
          Length = 1189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RC+L+ +ME
Sbjct: 1054 RCSLEGLME 1062
>XP_005263618
          Length = 146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CNLKLIME 9
           CN  LI+E
Sbjct: 134 CNYMLILE 141
>XP_005263614
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CNLKLIME 9
           CN  LI+E
Sbjct: 194 CNYMLILE 201
>XP_005263613
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CNLKLIME 9
           CN  LI+E
Sbjct: 194 CNYMLILE 201
>XP_005263612
          Length = 210

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CNLKLIME 9
           CN  LI+E
Sbjct: 198 CNYMLILE 205
>XP_005263609
          Length = 240

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CNLKLIME 9
           CN  LI+E
Sbjct: 228 CNYMLILE 235
>XP_005263607
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   CNLKLIME 9
           CN  LI+E
Sbjct: 288 CNYMLILE 295
>XP_005273407
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CNLKLI 7
           CN+KL+
Sbjct: 706 CNIKLL 711
>XP_006711903
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   CNLKLI 7
           CN+KL+
Sbjct: 707 CNIKLL 712
>NP_942090
          Length = 1189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    RCNLKLIME 9
            RC+L+ +ME
Sbjct: 1054 RCSLEGLME 1062
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.145    0.447 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 490,786
Number of extensions: 621
Number of successful extensions: 52
Number of sequences better than 10000.0: 52
Number of HSP's gapped: 52
Number of HSP's successfully gapped: 52
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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