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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              19     665
NP_001263689                                                           19     665
NP_001119584                                                           19     665
NP_001119586                                                           19     665
NP_001263625                                                           19     665
NP_001119590                                                           19     665
NP_001263690                                                           19     665
NP_001119585                                                           19     665
NP_001263624                                                           19     665
NP_001119588                                                           19     665
NP_001119587                                                           19     665
NP_001119589                                                           19     665
NP_955452                                                              17    4312
XP_006713543                                                           17    4312
NP_055448                                                              17    4312
NP_004332                                                              16    5631
NP_149094                                                              16    5631
XP_005264615                                                           16    5631
XP_006712164                                                           16    5631
XP_005264614                                                           16    5631
XP_005264613                                                           16    5631
XP_005264612                                                           16    5631
NP_001263627                                                           16    7355
NP_001263628                                                           16    7355
NP_001263626                                                           16    7355
NP_001164663                                                           16    7355
NP_001003698                                                           15    9605
NP_001161816                                                           15    9605
NP_001003699                                                           15    9605
NP_001003700                                                           15    9605
NP_056078                                                              15    9605
NP_001167587                                                           15    9605
NP_001167588                                                           15    9605
XP_005254313                                                           15    9605
XP_005254312                                                           15    9605
XP_006715224                                                           15    9605
XP_005249329                                                           15    9605
XP_006715223                                                           15    9605
XP_006715222                                                           15    9605
XP_005249333                                                           15    9605
XP_006715221                                                           15    9605
XP_006715220                                                           15    9605
XP_005249332                                                           15    9605
XP_006715219                                                           15    9605

>NP_000537
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263689
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119584
          Length = 393

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001119586
          Length = 341

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263625
          Length = 302

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119590
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001263690
          Length = 354

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119585
          Length = 346

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 158 RAMAIYKQS 166



>NP_001263624
          Length = 307

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   RAMAIHKQS 9
           RAMAI+KQS
Sbjct: 119 RAMAIYKQS 127



>NP_001119588
          Length = 209

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  RAMAIHKQS 9
          RAMAI+KQS
Sbjct: 26 RAMAIYKQS 34



>NP_001119587
          Length = 261

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  RAMAIHKQS 9
          RAMAI+KQS
Sbjct: 26 RAMAIYKQS 34



>NP_001119589
          Length = 214

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  RAMAIHKQS 9
          RAMAI+KQS
Sbjct: 26 RAMAIYKQS 34



>NP_955452
          Length = 2590

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAMAIHKQ 8
           R MAIHK+
Sbjct: 711 RQMAIHKR 718



>XP_006713543
          Length = 2591

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAMAIHKQ 8
           R MAIHK+
Sbjct: 712 RQMAIHKR 719



>NP_055448
          Length = 1173

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RAMAIHKQ 8
           RA+AI+KQ
Sbjct: 631 RALAIYKQ 638



>NP_004332
          Length = 2225

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    AMAIHKQ 8
            AMA+H+Q
Sbjct: 1024 AMALHRQ 1030



>NP_149094
          Length = 686

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAMAIHKQS 9
           RAMA H++S
Sbjct: 415 RAMAHHEES 423



>XP_005264615
          Length = 2162

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMAIHKQ 8
           AMA+H+Q
Sbjct: 961 AMALHRQ 967



>XP_006712164
          Length = 2179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AMAIHKQ 8
           AMA+H+Q
Sbjct: 961 AMALHRQ 967



>XP_005264614
          Length = 2191

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    AMAIHKQ 8
            AMA+H+Q
Sbjct: 1024 AMALHRQ 1030



>XP_005264613
          Length = 2208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    AMAIHKQ 8
            AMA+H+Q
Sbjct: 1024 AMALHRQ 1030



>XP_005264612
          Length = 2242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    AMAIHKQ 8
            AMA+H+Q
Sbjct: 1024 AMALHRQ 1030



>NP_001263627
          Length = 182

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 MAIHKQS 9
         MAI+KQS
Sbjct: 1 MAIYKQS 7



>NP_001263628
          Length = 187

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 MAIHKQS 9
         MAI+KQS
Sbjct: 1 MAIYKQS 7



>NP_001263626
          Length = 234

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3 MAIHKQS 9
         MAI+KQS
Sbjct: 1 MAIYKQS 7



>NP_001164663
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   AMAIHKQS 9
           AMA+HK +
Sbjct: 486 AMALHKDN 493



>NP_001003698
          Length = 1687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>NP_001161816
          Length = 1687

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>NP_001003699
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>NP_001003700
          Length = 1476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>NP_056078
          Length = 3036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RAMAIHKQS 9
            +A  IHK+S
Sbjct: 2652 KAKVIHKES 2660



>NP_001167587
          Length = 3037

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RAMAIHKQS 9
            +A  IHK+S
Sbjct: 2653 KAKVIHKES 2661



>NP_001167588
          Length = 2400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RAMAIHKQS 9
            +A  IHK+S
Sbjct: 2016 KAKVIHKES 2024



>XP_005254313
          Length = 3057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RAMAIHKQS 9
            +A  IHK+S
Sbjct: 2673 KAKVIHKES 2681



>XP_005254312
          Length = 3058

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    RAMAIHKQS 9
            +A  IHK+S
Sbjct: 2674 KAKVIHKES 2682



>XP_006715224
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_005249329
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_006715223
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_006715222
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_005249333
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_006715221
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_006715220
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_005249332
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



>XP_006715219
          Length = 1742

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   AMAIHKQS 9
           ++A+HKQ+
Sbjct: 330 SLALHKQT 337



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.325    0.117    0.308 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 479,226
Number of extensions: 411
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)