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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              18    1935
NP_055426                                                              17    4312
XP_005248757                                                           17    4312
XP_006715468                                                           17    4312
XP_005248756                                                           17    4312
XP_006715467                                                           17    4312
NP_065099                                                              16    7355
NP_076991                                                              16    7355
NP_002564                                                              16    7355
NP_001139412                                                           16    7355
NP_001139411                                                           16    7355
NP_001138985                                                           15    9605
NP_055581                                                              15    9605
NP_115791                                                              15    9605
NP_060345                                                              15    9605
NP_001269221                                                           15    9605
NP_009119                                                              15    9605
NP_001269220                                                           15    9605
NP_001035828                                                           15    9605
NP_001138987                                                           15    9605
NP_001138986                                                           15    9605
NP_001269222                                                           15    9605
XP_005249975                                                           15    9605
XP_006715858                                                           15    9605
XP_006715857                                                           15    9605
XP_006713277                                                           15    9605
XP_006711061                                                           15    9605
XP_006711060                                                           15    9605
XP_005244868                                                           15    9605
XP_003119848                                                           15    9605

>NP_004251
          Length = 1208

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   RALRSTLLR 9
           RALRS LLR
Sbjct: 367 RALRSRLLR 375



>NP_055426
          Length = 5596

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RALRSTLL 8
            RALR TLL
Sbjct: 4020 RALRETLL 4027



>XP_005248757
          Length = 3591

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RALRSTLL 8
            RALR TLL
Sbjct: 2015 RALRETLL 2022



>XP_006715468
          Length = 3744

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RALRSTLL 8
            RALR TLL
Sbjct: 2168 RALRETLL 2175



>XP_005248756
          Length = 3905

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RALRSTLL 8
            RALR TLL
Sbjct: 2329 RALRETLL 2336



>XP_006715467
          Length = 5550

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    RALRSTLL 8
            RALR TLL
Sbjct: 4020 RALRETLL 4027



>NP_065099
          Length = 1286

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RALRSTLLR 9
          + LR+TLLR
Sbjct: 72 KRLRTTLLR 80



>NP_076991
          Length = 562

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  RALRSTLLR 9
          RAL  TLLR
Sbjct: 53 RALTCTLLR 61



>NP_002564
          Length = 231

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RALRSTLLR 9
           RAL S LLR
Sbjct: 206 RALHSLLLR 214



>NP_001139412
          Length = 231

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RALRSTLLR 9
           RAL S LLR
Sbjct: 206 RALHSLLLR 214



>NP_001139411
          Length = 231

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   RALRSTLLR 9
           RAL S LLR
Sbjct: 206 RALHSLLLR 214



>NP_001138985
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           R LRST+L
Sbjct: 366 RKLRSTML 373



>NP_055581
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           R LRST+L
Sbjct: 366 RKLRSTML 373



>NP_115791
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RALRSTL 7
           RALR+TL
Sbjct: 866 RALRNTL 872



>NP_060345
          Length = 304

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           RALR TL+
Sbjct: 278 RALRRTLM 285



>NP_001269221
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RALRSTLLR 9
           RAL S+LLR
Sbjct: 212 RALFSSLLR 220



>NP_009119
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RALRSTLLR 9
           RAL S+LLR
Sbjct: 212 RALFSSLLR 220



>NP_001269220
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RALRSTLLR 9
           RAL S+LLR
Sbjct: 229 RALFSSLLR 237



>NP_001035828
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   RALRSTLL 8
           RAL++TLL
Sbjct: 514 RALQNTLL 521



>NP_001138987
          Length = 346

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           R LRST+L
Sbjct: 298 RKLRSTML 305



>NP_001138986
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           R LRST+L
Sbjct: 386 RKLRSTML 393



>NP_001269222
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   RALRSTLLR 9
           RAL S+LLR
Sbjct: 124 RALFSSLLR 132



>XP_005249975
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           R LRST+L
Sbjct: 366 RKLRSTML 373



>XP_006715858
          Length = 1267

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RALRSTL 7
           RALR+TL
Sbjct: 979 RALRNTL 985



>XP_006715857
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   RALRSTL 7
           RALR+TL
Sbjct: 980 RALRNTL 986



>XP_006713277
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RALRSTLL 8
           RALR TL+
Sbjct: 274 RALRRTLM 281



>XP_006711061
          Length = 1553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RALRSTLLR 9
            +A R+TLLR
Sbjct: 1095 QAARATLLR 1103



>XP_006711060
          Length = 1595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RALRSTLLR 9
            +A R+TLLR
Sbjct: 1137 QAARATLLR 1145



>XP_005244868
          Length = 1639

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    RALRSTLLR 9
            +A R+TLLR
Sbjct: 1181 QAARATLLR 1189



>XP_003119848
          Length = 515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RALRSTLLR 9
          +A R+TLLR
Sbjct: 57 QAARATLLR 65



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.141    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 273,126
Number of extensions: 337
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)