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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              19     665
XP_005271924                                                           19     665
XP_005271923                                                           19     665
NP_001379                                                              17    3301
XP_006721531                                                           17    3301
XP_005256556                                                           17    3301
NP_004138                                                              17    4312
NP_059107                                                              16    5631
NP_075013                                                              16    5631
NP_075014                                                              16    5631
NP_000335                                                              16    5631
NP_075015                                                              16    5631
NP_075012                                                              16    5631
NP_001243481                                                           16    5631
NP_078884                                                              16    5631
XP_005248632                                                           16    5631
XP_005276832                                                           16    5631
XP_006720745                                                           16    5631
XP_005254716                                                           16    5631
XP_006720744                                                           16    5631
XP_006720743                                                           16    5631
XP_006720742                                                           16    5631
XP_006720741                                                           16    5631
XP_006720740                                                           16    5631
XP_006714740                                                           16    5631
NP_060800                                                              16    7355
NP_001120683                                                           16    7355
NP_001245227                                                           16    7355
NP_001245228                                                           16    7355
NP_001245229                                                           16    7355
XP_005270059                                                           16    7355
XP_006717994                                                           16    7355
NP_005314                                                              15    9605

>NP_001261
          Length = 1710

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RAKKNKATK 9
            RAKKNKA K
Sbjct: 1335 RAKKNKAMK 1343



>XP_005271924
          Length = 1710

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RAKKNKATK 9
            RAKKNKA K
Sbjct: 1335 RAKKNKAMK 1343



>XP_005271923
          Length = 1798

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RAKKNKATK 9
            RAKKNKA K
Sbjct: 1335 RAKKNKAMK 1343



>NP_001379
          Length = 364

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RAKKNKATK 9
          R +KNKAT+
Sbjct: 16 RTQKNKATE 24



>XP_006721531
          Length = 312

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RAKKNKATK 9
          R +KNKAT+
Sbjct: 16 RTQKNKATE 24



>XP_005256556
          Length = 385

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RAKKNKATK 9
          R +KNKAT+
Sbjct: 16 RTQKNKATE 24



>NP_004138
          Length = 367

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  RAKKNKAT 8
          R +KNKAT
Sbjct: 17 RTQKNKAT 24



>NP_059107
          Length = 294

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_075013
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_075014
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_000335
          Length = 294

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_075015
          Length = 250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_075012
          Length = 262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_001243481
          Length = 476

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 382 RKKKSKATQ 390



>NP_078884
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 296 RKKKSKATQ 304



>XP_005248632
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>XP_005276832
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>XP_006720745
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 296 RKKKSKATQ 304



>XP_005254716
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 296 RKKKSKATQ 304



>XP_006720744
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 296 RKKKSKATQ 304



>XP_006720743
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 296 RKKKSKATQ 304



>XP_006720742
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 339 RKKKSKATQ 347



>XP_006720741
          Length = 436

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 342 RKKKSKATQ 350



>XP_006720740
          Length = 467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   RAKKNKATK 9
           R KK+KAT+
Sbjct: 373 RKKKSKATQ 381



>XP_006714740
          Length = 282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  AKKNKATK 9
          AKKNK+ K
Sbjct: 75 AKKNKSQK 82



>NP_060800
          Length = 456

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 388 AKKEKATQ 395



>NP_001120683
          Length = 631

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 388 AKKEKATQ 395



>NP_001245227
          Length = 571

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 328 AKKEKATQ 335



>NP_001245228
          Length = 558

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 388 AKKEKATQ 395



>NP_001245229
          Length = 498

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 328 AKKEKATQ 335



>XP_005270059
          Length = 573

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 388 AKKEKATQ 395



>XP_006717994
          Length = 617

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AKKNKATK 9
           AKK KAT+
Sbjct: 388 AKKEKATQ 395



>NP_005314
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   RAKKNKAT 8
           RAKK +AT
Sbjct: 152 RAKKPRAT 159



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.295    0.104    0.246 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 397,345
Number of extensions: 441
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)