Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
XP_005255358                                                           18    1482
XP_005255356                                                           18    1482
XP_005255355                                                           18    1482
NP_001138483                                                           18    1482
XP_005275744                                                           17    2528
NP_689824                                                              17    4312
XP_005257359                                                           17    4312
NP_005524                                                              17    4312
NP_009049                                                              16    5631
NP_001128653                                                           16    5631
NP_001011547                                                           16    5631
XP_006714558                                                           16    5631
NP_055833                                                              15    9605
NP_001186871                                                           15    9605
NP_055843                                                              15    9605
NP_001155038                                                           15    9605
NP_001155037                                                           15    9605
NP_001155036                                                           15    9605
NP_001155035                                                           15    9605
NP_001155034                                                           15    9605
NP_001155033                                                           15    9605
NP_001155032                                                           15    9605
XP_006715462                                                           15    9605
XP_006715461                                                           15    9605
XP_006715460                                                           15    9605
XP_006715459                                                           15    9605
XP_006715458                                                           15    9605
XP_006715457                                                           15    9605
XP_006715456                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RAIFTSEIG 9
            R IFTSEIG
Sbjct: 2940 RGIFTSEIG 2948
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    RAIFTSEIG 9
            R IFTSEIG
Sbjct: 2940 RGIFTSEIG 2948
>XP_005255358
          Length = 1086

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIFTSEIG 9
           A+FTSEIG
Sbjct: 227 AMFTSEIG 234
>XP_005255356
          Length = 1114

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIFTSEIG 9
           A+FTSEIG
Sbjct: 200 AMFTSEIG 207
>XP_005255355
          Length = 1121

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIFTSEIG 9
           A+FTSEIG
Sbjct: 227 AMFTSEIG 234
>NP_001138483
          Length = 1141

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   AIFTSEIG 9
           A+FTSEIG
Sbjct: 227 AMFTSEIG 234
>XP_005275744
          Length = 231

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   AIFTSEIG 9
           A+FTS+IG
Sbjct: 191 ALFTSQIG 198
>NP_689824
          Length = 740

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  RAIFTSEIG 9
          RA+ TSE+G
Sbjct: 88 RALSTSELG 96
>XP_005257359
          Length = 286

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AIFTSEIG 9
           A+FTSE G
Sbjct: 279 AVFTSESG 286
>NP_005524
          Length = 288

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AIFTSEIG 9
           A+FTSE G
Sbjct: 281 AVFTSESG 288
>NP_009049
          Length = 3097

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    IFTSEIG 9
            +FTSE+G
Sbjct: 1537 LFTSELG 1543
>NP_001128653
          Length = 706

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RAIFTSEIG 9
           RA+F  E+G
Sbjct: 365 RALFPDEVG 373
>NP_001011547
          Length = 681

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   RAIFTSEIG 9
           RA+F  E+G
Sbjct: 340 RALFPDEVG 348
>XP_006714558
          Length = 2612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    IFTSEIG 9
            +FTSE+G
Sbjct: 1052 LFTSELG 1058
>NP_055833
          Length = 2465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2184 AIFSSEI 2190
>NP_001186871
          Length = 2476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2175 AIFSSEI 2181
>NP_055843
          Length = 1360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            A+FTSE+
Sbjct: 1000 ALFTSEL 1006
>NP_001155038
          Length = 1268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 908 ALFTSEL 914
>NP_001155037
          Length = 1276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 916 ALFTSEL 922
>NP_001155036
          Length = 1297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 937 ALFTSEL 943
>NP_001155035
          Length = 1305

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 945 ALFTSEL 951
>NP_001155034
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 963 ALFTSEL 969
>NP_001155033
          Length = 1331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 971 ALFTSEL 977
>NP_001155032
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   AIFTSEI 8
           A+FTSE+
Sbjct: 992 ALFTSEL 998
>XP_006715462
          Length = 2452

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2151 AIFSSEI 2157
>XP_006715461
          Length = 2456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2175 AIFSSEI 2181
>XP_006715460
          Length = 2465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2184 AIFSSEI 2190
>XP_006715459
          Length = 2475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2174 AIFSSEI 2180
>XP_006715458
          Length = 2476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2175 AIFSSEI 2181
>XP_006715457
          Length = 2485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2184 AIFSSEI 2190
>XP_006715456
          Length = 2485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    AIFTSEI 8
            AIF+SEI
Sbjct: 2184 AIFSSEI 2190
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.326    0.141    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,943
Number of extensions: 481
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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