Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006501                                                              17    3301
NP_003974                                                              17    3301
NP_001070253                                                           17    3301
NP_008970                                                              17    3301
XP_005267344                                                           17    3301
NP_055726                                                              16    5631
NP_060063                                                              16    5631
NP_942595                                                              16    5631
NP_058641                                                              16    5631
XP_005263174                                                           16    5631
NP_001119577                                                           16    7355
NP_001119578                                                           16    7355
NP_078858                                                              16    7355
NP_001278232                                                           16    7355
NP_001278214                                                           16    7355
NP_001018081                                                           16    7355
NP_001137420                                                           16    7355
NP_116133                                                              16    7355
NP_078904                                                              16    7355
NP_001182412                                                           16    7355
XP_006720365                                                           16    7355
XP_005250261                                                           16    7355
XP_005249511                                                           16    7355
NP_690852                                                              16    7355
NP_003256                                                              15    9605
NP_002604                                                              15    9605
NP_001248745                                                           15    9605
NP_001171696                                                           15    9605
NP_919431                                                              15    9605
NP_699177                                                              15    9605
XP_006724525                                                           15    9605
XP_005255413                                                           15    9605
XP_006714357                                                           15    9605
XP_005275825                                                           15    9605
XP_003960932                                                           15    9605
XP_006710060                                                           15    9605
>NP_006501
          Length = 513

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   YFSLSY 9
           YFSLSY
Sbjct: 473 YFSLSY 478
>NP_003974
          Length = 515

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSYFSLSY 9
           ++YFS SY
Sbjct: 390 INYFSFSY 397
>NP_001070253
          Length = 515

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSYFSLSY 9
           ++YFS SY
Sbjct: 390 INYFSFSY 397
>NP_008970
          Length = 1174

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QVSYFSLSY 9
          +V+YFSL Y
Sbjct: 60 EVTYFSLWY 68
>XP_005267344
          Length = 1174

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QVSYFSLSY 9
          +V+YFSL Y
Sbjct: 60 EVTYFSLWY 68
>NP_055726
          Length = 961

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QVSYFS 6
           QVSYFS
Sbjct: 306 QVSYFS 311
>NP_060063
          Length = 662

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVSYFSLSY 9
           QVSYF+  +
Sbjct: 310 QVSYFAFKF 318
>NP_942595
          Length = 1161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVSYFSLSY 9
           QVSYF+  +
Sbjct: 310 QVSYFAFKF 318
>NP_058641
          Length = 291

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SYFSLSY 9
          SYF+L+Y
Sbjct: 70 SYFNLNY 76
>XP_005263174
          Length = 1124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVSYFSLSY 9
           QVSYF+  +
Sbjct: 310 QVSYFAFKF 318
>NP_001119577
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VSYFSLSY 9
           ++Y+S SY
Sbjct: 382 INYYSFSY 389
>NP_001119578
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VSYFSLSY 9
           ++Y+S SY
Sbjct: 382 INYYSFSY 389
>NP_078858
          Length = 4981

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SYFSLS 8
            SYFSLS
Sbjct: 3556 SYFSLS 3561
>NP_001278232
          Length = 4983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SYFSLS 8
            SYFSLS
Sbjct: 3558 SYFSLS 3563
>NP_001278214
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    SYFSLS 8
            SYFSLS
Sbjct: 3558 SYFSLS 3563
>NP_001018081
          Length = 1309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QVSYFSLSY 9
           +++YF L+Y
Sbjct: 379 ELTYFGLAY 387
>NP_001137420
          Length = 248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SYFSLSY 9
           ++FSLSY
Sbjct: 213 AFFSLSY 219
>NP_116133
          Length = 230

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SYFSLSY 9
           ++FSLSY
Sbjct: 195 AFFSLSY 201
>NP_078904
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SYFSLS 8
           SYFSLS
Sbjct: 401 SYFSLS 406
>NP_001182412
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   SYFSLS 8
           SYFSLS
Sbjct: 401 SYFSLS 406
>XP_006720365
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VSYFSLSY 9
           ++Y+S SY
Sbjct: 382 INYYSFSY 389
>XP_005250261
          Length = 495

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QVSYFSLS 8
           +++YFSLS
Sbjct: 401 ELAYFSLS 408
>XP_005249511
          Length = 230

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SYFSLSY 9
           ++FSLSY
Sbjct: 195 AFFSLSY 201
>NP_690852
          Length = 647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QVSYFSLS 8
           +++YFSLS
Sbjct: 553 ELAYFSLS 560
>NP_003256
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   QVSYFSLSY 9
           Q+ YF L Y
Sbjct: 299 QLEYFFLEY 307
>NP_002604
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLSY 9
           YF LSY
Sbjct: 156 YFGLSY 161
>NP_001248745
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLSY 9
           YF LSY
Sbjct: 156 YFGLSY 161
>NP_001171696
          Length = 645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVSYFSLSY 9
           Q+SY ++SY
Sbjct: 307 QMSYGTMSY 315
>NP_919431
          Length = 1717

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QVSYFSLSY 9
           Q+SY  LS+
Sbjct: 761 QLSYLGLSF 769
>NP_699177
          Length = 799

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QVSYFSLSY 9
           Q+SY ++SY
Sbjct: 398 QMSYGTMSY 406
>XP_006724525
          Length = 434

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QVSYFSLSY 9
          Q+SY ++SY
Sbjct: 33 QMSYGTMSY 41
>XP_005255413
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLSY 9
           YF LSY
Sbjct: 129 YFGLSY 134
>XP_006714357
          Length = 904

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   QVSYFSLSY 9
           Q+ YF L Y
Sbjct: 299 QLEYFFLEY 307
>XP_005275825
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLSY 9
           YF LSY
Sbjct: 129 YFGLSY 134
>XP_003960932
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLSY 9
           YF LSY
Sbjct: 129 YFGLSY 134
>XP_006710060
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   YFSLSY 9
           YF LSY
Sbjct: 129 YFGLSY 134
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.129    0.353 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 449,582
Number of extensions: 527
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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