Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_056160                                                              16    5631
NP_001188356                                                           16    5631
NP_055491                                                              16    5631
NP_001018494                                                           16    5631
XP_006715109                                                           16    5631
XP_006715108                                                           16    5631
XP_006715107                                                           16    5631
XP_006715106                                                           16    5631
XP_006715105                                                           16    5631
XP_006715104                                                           16    5631
XP_006715103                                                           16    5631
XP_006715102                                                           16    5631
XP_006712389                                                           16    5631
NP_001812                                                              16    7355
NP_001136245                                                           15    9605
NP_001136239                                                           15    9605
NP_001136236                                                           15    9605
NP_001136238                                                           15    9605
NP_001136240                                                           15    9605
NP_001136235                                                           15    9605
NP_001136237                                                           15    9605
NP_149045                                                              15    9605
NP_689534                                                              15    9605
NP_001073975                                                           15    9605
XP_005253360                                                           15    9605
XP_005253359                                                           15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QVSRFIELK 9
            QVSRFIEL+
Sbjct: 2033 QVSRFIELE 2041
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QVSRFIELK 9
            QVSRFIEL+
Sbjct: 2033 QVSRFIELE 2041
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QVSRFIELK 9
            QVSRFIEL+
Sbjct: 1986 QVSRFIELE 1994
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QVSRFIELK 9
            QVSRFIEL+
Sbjct: 2065 QVSRFIELE 2073
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QVSRFIELK 9
            QVSRFIEL+
Sbjct: 2078 QVSRFIELE 2086
>NP_056160
          Length = 1067

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>NP_001188356
          Length = 1068

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>NP_055491
          Length = 1186

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SRFIELK 9
           SRFI+LK
Sbjct: 863 SRFIQLK 869
>NP_001018494
          Length = 1188

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SRFIELK 9
           SRFI+LK
Sbjct: 863 SRFIQLK 869
>XP_006715109
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>XP_006715108
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>XP_006715107
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>XP_006715106
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>XP_006715105
          Length = 1077

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 145 VTRFIELE 152
>XP_006715104
          Length = 1111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 179 VTRFIELE 186
>XP_006715103
          Length = 1167

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 244 VTRFIELE 251
>XP_006715102
          Length = 1176

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSRFIELK 9
           V+RFIEL+
Sbjct: 244 VTRFIELE 251
>XP_006712389
          Length = 130

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QVSRFIEL 8
          Q+S FIEL
Sbjct: 32 QISSFIEL 39
>NP_001812
          Length = 656

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VSRFIELK 9
           VSR++E K
Sbjct: 253 VSRYVEFK 260
>NP_001136245
          Length = 1932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1654 VSRTVELK 1661
>NP_001136239
          Length = 1915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1637 VSRTVELK 1644
>NP_001136236
          Length = 1957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1679 VSRTVELK 1686
>NP_001136238
          Length = 1952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1674 VSRTVELK 1681
>NP_001136240
          Length = 1935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1657 VSRTVELK 1664
>NP_001136235
          Length = 1962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1684 VSRTVELK 1691
>NP_001136237
          Length = 1886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1608 VSRTVELK 1615
>NP_149045
          Length = 1955

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VSRFIELK 9
            VSR +ELK
Sbjct: 1677 VSRTVELK 1684
>NP_689534
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QVSRFIEL 8
           ++SRFIE+
Sbjct: 131 KLSRFIEI 138
>NP_001073975
          Length = 249

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  VSRFIELK 9
          V RFI+LK
Sbjct: 84 VLRFIQLK 91
>XP_005253360
          Length = 172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QVSRFIEL 8
          ++SRFIE+
Sbjct: 30 KLSRFIEI 37
>XP_005253359
          Length = 196

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QVSRFIEL 8
          ++SRFIE+
Sbjct: 54 KLSRFIEI 61
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.330    0.142    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 349,859
Number of extensions: 478
Number of successful extensions: 31
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 31
Number of HSP's successfully gapped: 31
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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