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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     390
NP_004561                                                              20     390
XP_005253450                                                           20     390
XP_006719151                                                           20     390
XP_006722357                                                           17    2528
NP_003046                                                              17    3301
NP_780775                                                              17    4312
NP_001230199                                                           17    4312
NP_055176                                                              17    4312
NP_653271                                                              16    7355
NP_852125                                                              16    7355
NP_001269353                                                           16    7355
NP_478137                                                              16    7355
NP_005640                                                              16    7355
XP_005253689                                                           16    7355
XP_006719761                                                           16    7355
NP_001177900                                                           16    7355
NP_004755                                                              16    7355
NP_065770                                                              15    9605

>NP_001275701
          Length = 1486

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  QVSLGFEQI 9
          QVSLGF+QI
Sbjct: 35 QVSLGFDQI 43



>NP_004561
          Length = 1445

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  QVSLGFEQI 9
          QVSLGF+QI
Sbjct: 35 QVSLGFDQI 43



>XP_005253450
          Length = 1486

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  QVSLGFEQI 9
          QVSLGF+QI
Sbjct: 35 QVSLGFDQI 43



>XP_006719151
          Length = 1486

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  QVSLGFEQI 9
          QVSLGF+QI
Sbjct: 35 QVSLGFDQI 43



>XP_006722357
          Length = 347

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   QVSLGFEQ 8
           QVSLGF Q
Sbjct: 181 QVSLGFPQ 188



>NP_003046
          Length = 532

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SLGFEQI 9
           SLGFEQ+
Sbjct: 445 SLGFEQL 451



>NP_780775
          Length = 1026

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QVSLGFEQI 9
           QVSLG+E +
Sbjct: 915 QVSLGWEPV 923



>NP_001230199
          Length = 1100

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QVSLGFEQI 9
           QVSLG+E +
Sbjct: 989 QVSLGWEPV 997



>NP_055176
          Length = 1100

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QVSLGFEQI 9
           QVSLG+E +
Sbjct: 989 QVSLGWEPV 997



>NP_653271
          Length = 1454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QVSLGF 6
           QVSLGF
Sbjct: 561 QVSLGF 566



>NP_852125
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QVSLGFE 7
           Q+SLGF+
Sbjct: 399 QISLGFK 405



>NP_001269353
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  QVSLGF 6
          QVSLGF
Sbjct: 70 QVSLGF 75



>NP_478137
          Length = 612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QVSLGFEQI 9
          QVSL FE +
Sbjct: 11 QVSLTFEDV 19



>NP_005640
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QVSLGFEQI 9
          QVSL FE +
Sbjct: 11 QVSLTFEDV 19



>XP_005253689
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  QVSLGFEQI 9
          Q+S GF+++
Sbjct: 66 QISAGFQEL 74



>XP_006719761
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  QVSLGFEQI 9
          Q+S GF+++
Sbjct: 66 QISAGFQEL 74



>NP_001177900
          Length = 829

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  QVSLGFEQI 9
          Q+S GF+++
Sbjct: 66 QISAGFQEL 74



>NP_004755
          Length = 861

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  QVSLGFEQI 9
          Q+S GF+++
Sbjct: 66 QISAGFQEL 74



>NP_065770
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   SLGFEQI 9
           SLGFE++
Sbjct: 202 SLGFERL 208



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.141    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 465,474
Number of extensions: 469
Number of successful extensions: 19
Number of sequences better than 10000.0: 19
Number of HSP's gapped: 19
Number of HSP's successfully gapped: 19
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)