Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001156685                                                           18    1135
NP_000133                                                              18    1135
XP_006713936                                                           18    1135
XP_006713935                                                           18    1135
XP_006713934                                                           18    1135
XP_006713933                                                           18    1135
NP_075254                                                              17    3301
XP_006713932                                                           17    4312
XP_006713931                                                           17    4312
NP_004230                                                              16    5631
NP_005286                                                              16    5631
XP_005268271                                                           16    5631
XP_005268270                                                           16    5631
XP_006720384                                                           16    5631
XP_006716001                                                           16    7355
NP_000836                                                              16    7355
NP_001107604                                                           15    9605
XP_006712765                                                           15    9605
XP_006712764                                                           15    9605
XP_005246844                                                           15    9605
XP_006712763                                                           15    9605
XP_006712762                                                           15    9605
XP_006712761                                                           15    9605
XP_005275790                                                           15    9605
>NP_001156685
          Length = 808

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           QVSLESN S
Sbjct: 424 QVSLESNAS 432
>NP_000133
          Length = 806

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           QVSLESN S
Sbjct: 422 QVSLESNAS 430
>XP_006713936
          Length = 807

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           QVSLESN S
Sbjct: 422 QVSLESNAS 430
>XP_006713935
          Length = 807

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           QVSLESN S
Sbjct: 423 QVSLESNAS 431
>XP_006713934
          Length = 809

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           QVSLESN S
Sbjct: 424 QVSLESNAS 432
>XP_006713933
          Length = 810

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           QVSLESN S
Sbjct: 425 QVSLESNAS 433
>NP_075254
          Length = 694

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVSLESNTS 9
           +VSLESN S
Sbjct: 310 KVSLESNAS 318
>XP_006713932
          Length = 810

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VSLESNTS 9
           VSLESN S
Sbjct: 427 VSLESNAS 434
>XP_006713931
          Length = 811

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   VSLESNTS 9
           VSLESN S
Sbjct: 427 VSLESNAS 434
>NP_004230
          Length = 1979

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QVSLESNT 8
           Q+SLE NT
Sbjct: 702 QLSLEKNT 709
>NP_005286
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   VSLESNTS 9
           +SLESNT+
Sbjct: 105 LSLESNTA 112
>XP_005268271
          Length = 1537

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QVSLESNT 8
           Q+SLE NT
Sbjct: 260 QLSLEKNT 267
>XP_005268270
          Length = 1950

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QVSLESNT 8
           Q+SLE NT
Sbjct: 673 QLSLEKNT 680
>XP_006720384
          Length = 1978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QVSLESNT 8
           Q+SLE NT
Sbjct: 701 QLSLEKNT 708
>XP_006716001
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SLESNTS 9
           SLE+NTS
Sbjct: 888 SLETNTS 894
>NP_000836
          Length = 908

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SLESNTS 9
           SLE+NTS
Sbjct: 888 SLETNTS 894
>NP_001107604
          Length = 2694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QVSLESN 7
           QVSLE N
Sbjct: 986 QVSLEKN 992
>XP_006712765
          Length = 2679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QVSLESN 7
           QVSLE N
Sbjct: 958 QVSLEKN 964
>XP_006712764
          Length = 2697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    QVSLESN 7
            QVSLE N
Sbjct: 1015 QVSLEKN 1021
>XP_005246844
          Length = 2707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QVSLESN 7
           QVSLE N
Sbjct: 986 QVSLEKN 992
>XP_006712763
          Length = 2716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    QVSLESN 7
            QVSLE N
Sbjct: 1015 QVSLEKN 1021
>XP_006712762
          Length = 2723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    QVSLESN 7
            QVSLE N
Sbjct: 1015 QVSLEKN 1021
>XP_006712761
          Length = 2736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1    QVSLESN 7
            QVSLE N
Sbjct: 1015 QVSLEKN 1021
>XP_005275790
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QVSLESNT 8
           QVSL  NT
Sbjct: 104 QVSLSENT 111
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.277   0.0942    0.199 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 338,175
Number of extensions: 317
Number of successful extensions: 24
Number of sequences better than 10000.0: 24
Number of HSP's gapped: 24
Number of HSP's successfully gapped: 24
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.6 bits)
S2: 28 (15.4 bits)

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