Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     509
NP_004561                                                              20     509
XP_005253450                                                           20     509
XP_006719151                                                           20     509
NP_689824                                                              17    2528
NP_001230085                                                           17    4312
NP_005670                                                              17    4312
NP_647610                                                              17    4312
NP_001230089                                                           17    4312
NP_001230088                                                           17    4312
NP_001230087                                                           17    4312
NP_001230086                                                           17    4312
NP_060862                                                              17    4312
XP_006721392                                                           17    4312
XP_006721391                                                           17    4312
XP_005256284                                                           17    4312
XP_006721389                                                           17    4312
XP_005256283                                                           17    4312
XP_006721388                                                           17    4312
XP_005263172                                                           17    4312
XP_006725527                                                           16    5631
XP_006726289                                                           16    5631
XP_006726288                                                           16    5631
XP_006714809                                                           16    5631
XP_006710031                                                           16    5631
NP_778205                                                              16    7355
NP_778204                                                              16    7355
NP_008858                                                              16    7355
NP_477515                                                              16    7355
NP_077274                                                              16    7355
NP_001139555                                                           16    7355
NP_001139554                                                           16    7355
NP_001139528                                                           16    7355
NP_919440                                                              16    7355
NP_919439                                                              16    7355
XP_005251979                                                           16    7355
XP_006710397                                                           16    7355
XP_005244776                                                           16    7355
XP_006710396                                                           16    7355
XP_005244775                                                           16    7355
NP_849150                                                              16    7355
NP_919296                                                              16    7355
NP_001181915                                                           15    9605
XP_006718995                                                           15    9605
XP_005274425                                                           15    9605
XP_006718994                                                           15    9605
XP_006718993                                                           15    9605
XP_005274424                                                           15    9605
XP_005274423                                                           15    9605
XP_006710703                                                           15    9605
NP_203753                                                              15    9605
>NP_001275701
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSKAPTIG 9
           QVSKAP IG
Sbjct: 100 QVSKAPAIG 108
>NP_004561
          Length = 1445

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSKAPTIG 9
           QVSKAP IG
Sbjct: 100 QVSKAPAIG 108
>XP_005253450
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSKAPTIG 9
           QVSKAP IG
Sbjct: 100 QVSKAPAIG 108
>XP_006719151
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVSKAPTIG 9
           QVSKAP IG
Sbjct: 100 QVSKAPAIG 108
>NP_689824
          Length = 740

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VSKAPTIG 9
           VSKAP +G
Sbjct: 406 VSKAPNVG 413
>NP_001230085
          Length = 843

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 536 KAPTIG 541
>NP_005670
          Length = 869

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 562 KAPTIG 567
>NP_647610
          Length = 775

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 468 KAPTIG 473
>NP_001230089
          Length = 392

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  KAPTIG 9
          KAPTIG
Sbjct: 85 KAPTIG 90
>NP_001230088
          Length = 460

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 153 KAPTIG 158
>NP_001230087
          Length = 537

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 230 KAPTIG 235
>NP_001230086
          Length = 537

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 230 KAPTIG 235
>NP_060862
          Length = 2104

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SKAPTIG 9
            S+APTIG
Sbjct: 1678 SEAPTIG 1684
>XP_006721392
          Length = 744

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 437 KAPTIG 442
>XP_006721391
          Length = 744

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 437 KAPTIG 442
>XP_005256284
          Length = 802

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 495 KAPTIG 500
>XP_006721389
          Length = 844

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 537 KAPTIG 542
>XP_005256283
          Length = 869

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 562 KAPTIG 567
>XP_006721388
          Length = 870

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   KAPTIG 9
           KAPTIG
Sbjct: 563 KAPTIG 568
>XP_005263172
          Length = 2104

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    SKAPTIG 9
            S+APTIG
Sbjct: 1678 SEAPTIG 1684
>XP_006725527
          Length = 1322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 394 VSKAPTM 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 662 VSKAPTM 668

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 933 VSKAPTM 939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    VSKAPTI 8
            VSKAPT+
Sbjct: 1204 VSKAPTM 1210
>XP_006726289
          Length = 900

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 236 VSKAPTM 242

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 507 VSKAPTM 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 779 VSKAPTM 785
>XP_006726288
          Length = 659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 266 VSKAPTM 272

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 538 VSKAPTM 544
>XP_006714809
          Length = 1574

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 394 VSKAPTM 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 662 VSKAPTM 668

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VSKAPTI 8
           VSKAPT+
Sbjct: 933 VSKAPTM 939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    VSKAPTI 8
            VSKAPT+
Sbjct: 1205 VSKAPTM 1211

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    VSKAPTI 8
            VSKAPT+
Sbjct: 1476 VSKAPTM 1482
>XP_006710031
          Length = 295

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  SKAPTIG 9
          S+APT+G
Sbjct: 64 SRAPTLG 70
>NP_778205
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  QVSKAPTIG 9
          ++++APT+G
Sbjct: 18 RMNQAPTVG 26
>NP_778204
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   QVSKAPTIG 9
           ++++APT+G
Sbjct: 102 RMNQAPTVG 110
>NP_008858
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   QVSKAPTIG 9
           ++++APT+G
Sbjct: 178 RMNQAPTVG 186
>NP_477515
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VSKAPTIG 9
           V K PT+G
Sbjct: 123 VEKGPTLG 130
>NP_077274
          Length = 472

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SKAPTIG 9
           SK PT+G
Sbjct: 135 SKGPTLG 141
>NP_001139555
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SKAPTIG 9
          SK PT+G
Sbjct: 62 SKGPTLG 68
>NP_001139554
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SKAPTIG 9
           SK PT+G
Sbjct: 135 SKGPTLG 141
>NP_001139528
          Length = 399

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SKAPTIG 9
          SK PT+G
Sbjct: 62 SKGPTLG 68
>NP_919440
          Length = 207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VSKAPTIG 9
          V K PT+G
Sbjct: 71 VEKGPTLG 78
>NP_919439
          Length = 207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VSKAPTIG 9
          V K PT+G
Sbjct: 71 VEKGPTLG 78
>XP_005251979
          Length = 216

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   QVSKAPTIG 9
           ++++APT+G
Sbjct: 102 RMNQAPTVG 110
>XP_006710397
          Length = 180

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VSKAPTIG 9
          V K PT+G
Sbjct: 44 VEKGPTLG 51
>XP_005244776
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VSKAPTIG 9
           V K PT+G
Sbjct: 123 VEKGPTLG 130
>XP_006710396
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VSKAPTIG 9
           V K PT+G
Sbjct: 123 VEKGPTLG 130
>XP_005244775
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VSKAPTIG 9
           V K PT+G
Sbjct: 123 VEKGPTLG 130
>NP_849150
          Length = 1445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QVSKAPTIG 9
           QVS A T+G
Sbjct: 421 QVSDATTVG 429
>NP_919296
          Length = 275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   VSKAPTIG 9
           V K PT+G
Sbjct: 139 VEKGPTLG 146
>NP_001181915
          Length = 517

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SKAPTIG 9
           S APT+G
Sbjct: 490 SSAPTLG 496
>XP_006718995
          Length = 2382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 189 SKAPTV 194
>XP_005274425
          Length = 2566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 422 SKAPTV 427
>XP_006718994
          Length = 2567

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 422 SKAPTV 427
>XP_006718993
          Length = 2602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 422 SKAPTV 427
>XP_005274424
          Length = 2614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 422 SKAPTV 427
>XP_005274423
          Length = 2615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 422 SKAPTV 427
>XP_006710703
          Length = 552

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   SKAPTIG 9
           S APT+G
Sbjct: 525 SSAPTLG 531
>NP_203753
          Length = 2601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   SKAPTI 8
           SKAPT+
Sbjct: 422 SKAPTV 427
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.122    0.327 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 558,481
Number of extensions: 381
Number of successful extensions: 61
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 61
Number of HSP's successfully gapped: 61
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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