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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000213                                                              18    1935
NP_001087241                                                           18    1935
XP_005265799                                                           18    1935
XP_005265798                                                           18    1935
XP_005265797                                                           18    1935
NP_005202                                                              16    5631
NP_001004106                                                           16    5631
NP_001004105                                                           16    5631
NP_002073                                                              16    5631
NP_001275634                                                           16    5631
NP_055928                                                              16    5631
XP_005256618                                                           16    5631
XP_005256617                                                           16    5631
XP_006714922                                                           16    5631
XP_006714921                                                           16    5631
NP_003836                                                              16    7355
NP_653267                                                              16    7355
NP_689835                                                              16    7355
XP_005248505                                                           16    7355
NP_006019                                                              15    9605
NP_001269109                                                           15    9605
NP_006237                                                              15    9605
NP_001269111                                                           15    9605
NP_001269110                                                           15    9605
NP_001269108                                                           15    9605
NP_005289                                                              15    9605
NP_001129386                                                           15    9605
NP_060918                                                              15    9605

>NP_000213
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVAK MAFL
Sbjct: 775 QVAKGMAFL 783



>NP_001087241
          Length = 972

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVAK MAFL
Sbjct: 771 QVAKGMAFL 779



>XP_005265799
          Length = 973

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVAK MAFL
Sbjct: 772 QVAKGMAFL 780



>XP_005265798
          Length = 976

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVAK MAFL
Sbjct: 775 QVAKGMAFL 783



>XP_005265797
          Length = 977

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVAK MAFL
Sbjct: 776 QVAKGMAFL 784



>NP_005202
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVA+ MAFL
Sbjct: 761 QVAQGMAFL 769



>NP_001004106
          Length = 576

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  QVAKCMAFL 9
          ++++C+AFL
Sbjct: 76 ELSRCVAFL 84



>NP_001004105
          Length = 560

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  QVAKCMAFL 9
          ++++C+AFL
Sbjct: 76 ELSRCVAFL 84



>NP_002073
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  QVAKCMAFL 9
          ++++C+AFL
Sbjct: 76 ELSRCVAFL 84



>NP_001275634
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QVAKCMAFL 9
           QVA+ MAFL
Sbjct: 761 QVAQGMAFL 769



>NP_055928
          Length = 2961

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAKCMAFL 9
           VAKC A++
Sbjct: 249 VAKCYAYI 256



>XP_005256618
          Length = 2962

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAKCMAFL 9
           VAKC A++
Sbjct: 249 VAKCYAYI 256



>XP_005256617
          Length = 2966

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAKCMAFL 9
           VAKC A++
Sbjct: 249 VAKCYAYI 256



>XP_006714922
          Length = 557

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  QVAKCMAFL 9
          ++++C+AFL
Sbjct: 44 ELSRCVAFL 52



>XP_006714921
          Length = 559

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  QVAKCMAFL 9
          ++++C+AFL
Sbjct: 46 ELSRCVAFL 54



>NP_003836
          Length = 520

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   QVAKCM 6
           QVAKC+
Sbjct: 117 QVAKCL 122



>NP_653267
          Length = 4753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    KCMAF 8
            KCMAF
Sbjct: 1036 KCMAF 1040



>NP_689835
          Length = 549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VAKCMAF 8
           VA+C+AF
Sbjct: 313 VAECVAF 319



>XP_005248505
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VAKCMAF 8
           VA+C+AF
Sbjct: 314 VAECVAF 320



>NP_006019
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 5   CMAFL 9
           CMAFL
Sbjct: 309 CMAFL 313



>NP_001269109
          Length = 462

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   QVAKCMA 7
           Q+AKC++
Sbjct: 347 QIAKCVS 353



>NP_006237
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   QVAKCMA 7
           Q+AKC++
Sbjct: 347 QIAKCVS 353



>NP_001269111
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   QVAKCMA 7
           Q+AKC++
Sbjct: 271 QIAKCVS 277



>NP_001269110
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   QVAKCMA 7
           Q+AKC++
Sbjct: 271 QIAKCVS 277



>NP_001269108
          Length = 467

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   QVAKCMA 7
           Q+AKC++
Sbjct: 347 QIAKCVS 353



>NP_005289
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VAKCMAFL 9
           +A C+AF+
Sbjct: 291 IATCLAFV 298



>NP_001129386
          Length = 1503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 3    AKCMAF 8
            A CMAF
Sbjct: 1148 ASCMAF 1153



>NP_060918
          Length = 1230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VAKCMAFL 9
           +AKC+A L
Sbjct: 799 IAKCVAAL 806



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.347    0.136    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 477,780
Number of extensions: 419
Number of successful extensions: 28
Number of sequences better than 10000.0: 28
Number of HSP's gapped: 28
Number of HSP's successfully gapped: 28
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)