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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           19     665
NP_858045                                                              19     665
NP_006525                                                              19     665
NP_001167559                                                           19     665
NP_085135                                                              17    4312
XP_005258413                                                           17    4312
NP_078966                                                              16    5631
NP_001265506                                                           16    5631
NP_001265507                                                           16    5631
NP_001265505                                                           16    5631
NP_001265504                                                           16    5631
NP_001192202                                                           16    5631
XP_006723004                                                           16    5631
NP_001165074                                                           16    7355
NP_001165077                                                           16    7355
NP_001035845                                                           16    7355
NP_006027                                                              16    7355
NP_001035844                                                           16    7355
NP_001003801                                                           16    7355
XP_005264714                                                           16    7355
XP_005264712                                                           16    7355
XP_005264711                                                           16    7355
XP_006712215                                                           16    7355
XP_006712214                                                           16    7355
XP_005264710                                                           16    7355
NP_112174                                                              16    7355
NP_006531                                                              15    9605
NP_061927                                                              15    9605
XP_006717968                                                           15    9605
XP_006717967                                                           15    9605
XP_006717966                                                           15    9605
XP_006717965                                                           15    9605
XP_005251190                                                           15    9605
XP_005251188                                                           15    9605
XP_005251189                                                           15    9605
XP_005251187                                                           15    9605
XP_005251186                                                           15    9605
XP_005251185                                                           15    9605

>NP_001167558
          Length = 1423

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QTKSKLFLN 9
           QTKSKLF N
Sbjct: 346 QTKSKLFRN 354



>NP_858045
          Length = 1424

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QTKSKLFLN 9
           QTKSKLF N
Sbjct: 346 QTKSKLFRN 354



>NP_006525
          Length = 1420

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QTKSKLFLN 9
           QTKSKLF N
Sbjct: 346 QTKSKLFRN 354



>NP_001167559
          Length = 1415

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QTKSKLFLN 9
           QTKSKLF N
Sbjct: 356 QTKSKLFRN 364



>NP_085135
          Length = 2248

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    QTKSKLF 7
            QTK+KLF
Sbjct: 1105 QTKAKLF 1111



>XP_005258413
          Length = 2249

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    QTKSKLF 7
            QTK+KLF
Sbjct: 1106 QTKAKLF 1112



>NP_078966
          Length = 14507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    QTKSKLFL 8
            QTKSKL L
Sbjct: 9279 QTKSKLSL 9286



>NP_001265506
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TKSKLFL 8
           TK+KLFL
Sbjct: 105 TKAKLFL 111



>NP_001265507
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TKSKLFL 8
           TK+KLFL
Sbjct: 105 TKAKLFL 111



>NP_001265505
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TKSKLFL 8
           TK+KLFL
Sbjct: 105 TKAKLFL 111



>NP_001265504
          Length = 140

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TKSKLFL 8
           TK+KLFL
Sbjct: 105 TKAKLFL 111



>NP_001192202
          Length = 148

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   TKSKLFL 8
           TK+KLFL
Sbjct: 113 TKAKLFL 119



>XP_006723004
          Length = 6443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1    QTKSKLFL 8
            QTKSKL L
Sbjct: 1193 QTKSKLSL 1200



>NP_001165074
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>NP_001165077
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>NP_001035845
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>NP_006027
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>NP_001035844
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>NP_001003801
          Length = 483

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  TKSKLF 7
          TKSKLF
Sbjct: 14 TKSKLF 19



>XP_005264714
          Length = 678

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>XP_005264712
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>XP_005264711
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>XP_006712215
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>XP_006712214
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>XP_005264710
          Length = 738

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1 QTKSKLFL 8
         Q K+KLFL
Sbjct: 2 QQKTKLFL 9



>NP_112174
          Length = 551

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SKLFLN 9
           SKLFLN
Sbjct: 330 SKLFLN 335



>NP_006531
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



>NP_061927
          Length = 835

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTKSKLFL 8
           +TK K+FL
Sbjct: 481 ETKKKVFL 488



>XP_006717968
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTKSKLFL 8
           +TK K+FL
Sbjct: 481 ETKKKVFL 488



>XP_006717967
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTKSKLFL 8
           +TK K+FL
Sbjct: 481 ETKKKVFL 488



>XP_006717966
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTKSKLFL 8
           +TK K+FL
Sbjct: 481 ETKKKVFL 488



>XP_006717965
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTKSKLFL 8
           +TK K+FL
Sbjct: 487 ETKKKVFL 494



>XP_005251190
          Length = 1395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



>XP_005251188
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



>XP_005251189
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



>XP_005251187
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



>XP_005251186
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



>XP_005251185
          Length = 1464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QTKSKL 6
           QTKSKL
Sbjct: 350 QTKSKL 355



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.126    0.318 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 386,380
Number of extensions: 653
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)