Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              18    1135
NP_004546                                                              17    2528
NP_775186                                                              17    2528
NP_775188                                                              17    2528
NP_065928                                                              17    3301
XP_005256527                                                           17    3301
NP_060557                                                              17    4312
XP_006710825                                                           17    4312
NP_001162023                                                           17    4312
NP_065852                                                              16    7355
NP_775766                                                              15    9605
NP_001239049                                                           15    9605
NP_055963                                                              15    9605
NP_149107                                                              15    9605
NP_001239053                                                           15    9605
NP_001239051                                                           15    9605
NP_001253981                                                           15    9605
NP_001239048                                                           15    9605
XP_005257983                                                           15    9605
XP_005268266                                                           15    9605
XP_006720366                                                           15    9605
XP_005268265                                                           15    9605
XP_005247048                                                           15    9605
XP_005247047                                                           15    9605
NP_663748                                                              15    9605
NP_775259                                                              15    9605
NP_001028748                                                           15    9605

>NP_002430
          Length = 1137

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QTISGIVNL 9
           Q ISGIVNL
Sbjct: 487 QIISGIVNL 495



>NP_004546
          Length = 1068

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TISGIVNL 9
           T+SG+VNL
Sbjct: 837 TLSGLVNL 844



>NP_775186
          Length = 1065

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TISGIVNL 9
           T+SG+VNL
Sbjct: 837 TLSGLVNL 844



>NP_775188
          Length = 1075

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   TISGIVNL 9
           T+SG+VNL
Sbjct: 837 TLSGLVNL 844



>NP_065928
          Length = 4427

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QTISGIVN 8
           QT++G+VN
Sbjct: 887 QTLAGVVN 894



>XP_005256527
          Length = 4427

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QTISGIVN 8
           QT++G+VN
Sbjct: 887 QTLAGVVN 894



>NP_060557
          Length = 674

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TISGIVNL 9
           T+SG++NL
Sbjct: 260 TVSGLLNL 267



>XP_006710825
          Length = 669

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TISGIVNL 9
           T+SG++NL
Sbjct: 255 TVSGLLNL 262



>NP_001162023
          Length = 634

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   TISGIVNL 9
           T+SG++NL
Sbjct: 220 TVSGLLNL 227



>NP_065852
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2  TISGIVNL 9
          T+SG VNL
Sbjct: 29 TVSGRVNL 36



>NP_775766
          Length = 888

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           T +GI+NL
Sbjct: 313 TAAGIINL 320



>NP_001239049
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 344 TISGLITL 351



>NP_055963
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 344 TISGLITL 351



>NP_149107
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTISGIVN 8
           Q +SGI+N
Sbjct: 568 QCMSGIIN 575



>NP_001239053
          Length = 1143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 344 TISGLITL 351



>NP_001239051
          Length = 1288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 309 TISGLITL 316



>NP_001253981
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QTISGIV 7
           QT+SGI+
Sbjct: 400 QTVSGIL 406



>NP_001239048
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 344 TISGLITL 351



>XP_005257983
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QTISGIV 7
           QT+SGI+
Sbjct: 400 QTVSGIL 406



>XP_005268266
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTISGIVN 8
           Q +SGI+N
Sbjct: 462 QCMSGIIN 469



>XP_006720366
          Length = 971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTISGIVN 8
           Q +SGI+N
Sbjct: 580 QCMSGIIN 587



>XP_005268265
          Length = 991

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QTISGIVN 8
           Q +SGI+N
Sbjct: 580 QCMSGIIN 587



>XP_005247048
          Length = 1107

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 128 TISGLITL 135



>XP_005247047
          Length = 1137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TISGIVNL 9
           TISG++ L
Sbjct: 158 TISGLITL 165



>NP_663748
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QTISGIVN 8
          QT+S +VN
Sbjct: 45 QTVSQVVN 52



>NP_775259
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QTISGIV 7
           QT+SGI+
Sbjct: 400 QTVSGIL 406



>NP_001028748
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QTISGIV 7
           QT+SGI+
Sbjct: 400 QTVSGIL 406



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.136    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 408,051
Number of extensions: 537
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)