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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_002430                                                              20     390
NP_005712                                                              16    7355
NP_006288                                                              15    9605
NP_852468                                                              15    9605
NP_598395                                                              15    9605
NP_001025226                                                           15    9605
NP_001164                                                              15    9605
NP_001192246                                                           15    9605
NP_006678                                                              15    9605
XP_005267693                                                           15    9605
XP_005267692                                                           15    9605
NP_892015                                                              15    9605

>NP_002430
          Length = 1137

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QTAKVGDKT 9
           Q AKVGDKT
Sbjct: 692 QAAKVGDKT 700



>NP_005712
          Length = 418

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QTAKVGDK 8
          ++AKVGD+
Sbjct: 39 ESAKVGDQ 46



>NP_006288
          Length = 633

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QTAKVGDKT 9
          + AK G+KT
Sbjct: 34 RAAKAGEKT 42



>NP_852468
          Length = 140

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QTAKVGDKT 9
          +TAK+ D+T
Sbjct: 38 KTAKIEDET 46



>NP_598395
          Length = 200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QTAKVGDKT 9
          +TAK+ D+T
Sbjct: 38 KTAKIEDET 46



>NP_001025226
          Length = 1502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    QTAKVGDKT 9
            + AK+ DKT
Sbjct: 1356 EAAKIPDKT 1364



>NP_001164
          Length = 1501

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    QTAKVGDKT 9
            + AK+ DKT
Sbjct: 1355 EAAKIPDKT 1363



>NP_001192246
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TAKVGDKT 9
           TAK G KT
Sbjct: 142 TAKPGSKT 149



>NP_006678
          Length = 435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QTAKVGD 7
           +TAK GD
Sbjct: 105 ETAKTGD 111



>XP_005267693
          Length = 1502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    QTAKVGDKT 9
            + AK+ DKT
Sbjct: 1356 EAAKIPDKT 1364



>XP_005267692
          Length = 1502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    QTAKVGDKT 9
            + AK+ DKT
Sbjct: 1356 EAAKIPDKT 1364



>NP_892015
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   TAKVGDKT 9
           TAK G KT
Sbjct: 141 TAKPGSKT 148



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.297    0.114    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 454,673
Number of extensions: 522
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)