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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1482
NP_000858                                                              16    5631
XP_005246577                                                           16    5631
XP_006712545                                                           16    5631
NP_001019849                                                           15    9605
NP_001129628                                                           15    9605
NP_055725                                                              15    9605

>NP_004295
          Length = 1620

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QSSSLAMLD 9
            Q SSLAMLD
Sbjct: 1217 QPSSLAMLD 1225



>NP_000858
          Length = 481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QSSSLAMLD 9
           QSSS+ +LD
Sbjct: 454 QSSSIILLD 462



>XP_005246577
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QSSSLAMLD 9
           QSSS+ +LD
Sbjct: 335 QSSSIILLD 343



>XP_006712545
          Length = 456

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QSSSLAMLD 9
           QSSS+ +LD
Sbjct: 429 QSSSIILLD 437



>NP_001019849
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SSLAMLD 9
           SSLA+LD
Sbjct: 170 SSLALLD 176



>NP_001129628
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  QSSSLAML 8
          +SSS+AML
Sbjct: 3  ESSSVAML 10



>NP_055725
          Length = 953

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  QSSSLAML 8
          +SSS+AML
Sbjct: 3  ESSSVAML 10



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.109    0.239 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 252,975
Number of extensions: 103
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)