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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005261689                                                           18    1482
XP_006724326                                                           18    1482
NP_115803                                                              17    2528
NP_057160                                                              16    5631
XP_005273211                                                           16    5631

>XP_005261689
          Length = 677

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  QSSISAQLQ 9
          Q+S+SAQLQ
Sbjct: 75 QASVSAQLQ 83



>XP_006724326
          Length = 717

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  QSSISAQLQ 9
          Q+S+SAQLQ
Sbjct: 75 QASVSAQLQ 83



>NP_115803
          Length = 1156

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QSSISAQLQ 9
           QSSISAQ Q
Sbjct: 621 QSSISAQQQ 629




 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QSSISAQLQ 9
           QSSISAQ Q
Sbjct: 633 QSSISAQQQ 641



>NP_057160
          Length = 194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QSSISAQLQ 9
           QSS+S +LQ
Sbjct: 156 QSSVSQELQ 164



>XP_005273211
          Length = 161

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QSSISAQLQ 9
           QSS+S +LQ
Sbjct: 123 QSSVSQELQ 131



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.100    0.204 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 230,502
Number of extensions: 164
Number of successful extensions: 6
Number of sequences better than 10000.0: 5
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)