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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           18    1135
NP_004561                                                              18    1135
NP_001275703                                                           18    1135
XP_005253450                                                           18    1135
XP_006719151                                                           18    1135
NP_001164268                                                           16    5631
NP_078975                                                              16    5631
NP_001164267                                                           16    5631
XP_005264950                                                           16    7355
XP_006713056                                                           16    7355
XP_005264949                                                           16    7355
NP_001035522                                                           16    7355
NP_055247                                                              15    9605
NP_001265651                                                           15    9605
NP_001265653                                                           15    9605
NP_001265652                                                           15    9605
XP_005274446                                                           15    9605
XP_006715569                                                           15    9605
XP_006715568                                                           15    9605
NP_006840                                                              15    9605

>NP_001275701
          Length = 1486

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QSSETSAK 8
           QSSETSAK
Sbjct: 469 QSSETSAK 476



>NP_004561
          Length = 1445

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QSSETSAK 8
           QSSETSAK
Sbjct: 469 QSSETSAK 476



>NP_001275703
          Length = 1264

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QSSETSAK 8
           QSSETSAK
Sbjct: 247 QSSETSAK 254



>XP_005253450
          Length = 1486

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QSSETSAK 8
           QSSETSAK
Sbjct: 469 QSSETSAK 476



>XP_006719151
          Length = 1486

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QSSETSAK 8
           QSSETSAK
Sbjct: 469 QSSETSAK 476



>NP_001164268
          Length = 227

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   QSSETSAKV 9
           ++SET+AKV
Sbjct: 135 KNSETAAKV 143



>NP_078975
          Length = 268

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   QSSETSAKV 9
           ++SET+AKV
Sbjct: 135 KNSETAAKV 143



>NP_001164267
          Length = 261

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1   QSSETSAKV 9
           ++SET+AKV
Sbjct: 135 KNSETAAKV 143



>XP_005264950
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSETSAKV 9
           SSE SAKV
Sbjct: 128 SSEDSAKV 135



>XP_006713056
          Length = 384

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSETSAKV 9
           SSE SAKV
Sbjct: 128 SSEDSAKV 135



>XP_005264949
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSETSAKV 9
           SSE SAKV
Sbjct: 128 SSEDSAKV 135



>NP_001035522
          Length = 356

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  SSETSAKV 9
          SSE SAKV
Sbjct: 45 SSEDSAKV 52



>NP_055247
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSSETSAKV 9
           QSSE  AK+
Sbjct: 243 QSSEFKAKI 251



>NP_001265651
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSSETSAKV 9
           QSSE  AK+
Sbjct: 194 QSSEFKAKI 202



>NP_001265653
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSSETSAKV 9
           QSSE  AK+
Sbjct: 110 QSSEFKAKI 118



>NP_001265652
          Length = 442

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSSETSAKV 9
           QSSE  AK+
Sbjct: 132 QSSEFKAKI 140



>XP_005274446
          Length = 415

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SSETSAK 8
           SSETSA+
Sbjct: 370 SSETSAR 376



>XP_006715569
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSSETSAKV 9
           QSSE  AK+
Sbjct: 110 QSSEFKAKI 118



>XP_006715568
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSSETSAKV 9
           QSSE  AK+
Sbjct: 221 QSSEFKAKI 229



>NP_006840
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SSETSAKV 9
           +S+TSAK+
Sbjct: 263 NSQTSAKI 270



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.268   0.0829    0.165 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 207,254
Number of extensions: 126
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 19 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.4 bits)
S2: 28 (15.4 bits)