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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006719294                                                           17    4312
NP_001138947                                                           17    4312
NP_079255                                                              16    5631
NP_001106203                                                           16    5631
NP_997297                                                              16    5631
XP_005268373                                                           16    5631
XP_005268372                                                           16    5631
XP_005268371                                                           16    5631
XP_006720031                                                           16    5631
XP_005268370                                                           16    5631
XP_006720030                                                           16    5631
XP_005268369                                                           16    5631
XP_006720029                                                           16    5631
XP_005268367                                                           16    5631
XP_005268366                                                           16    5631
XP_005268365                                                           16    5631
XP_005268364                                                           16    5631
XP_006715752                                                           16    7355
NP_001243084                                                           15    9605
NP_963889                                                              15    9605
NP_056943                                                              15    9605
NP_001273575                                                           15    9605
NP_001138397                                                           15    9605
NP_001269849                                                           15    9605
NP_001171517                                                           15    9605
NP_002453                                                              15    9605
NP_001139373                                                           15    9605
NP_060297                                                              15    9605
NP_001265869                                                           15    9605
NP_001274166                                                           15    9605
NP_060840                                                              15    9605
NP_002454                                                              15    9605
NP_001017924                                                           15    9605
NP_001271152                                                           15    9605
NP_001074472                                                           15    9605
NP_001074473                                                           15    9605
XP_005276308                                                           15    9605
XP_006724069                                                           15    9605
XP_005261041                                                           15    9605
XP_005261040                                                           15    9605
XP_005261039                                                           15    9605
XP_005261038                                                           15    9605
XP_005261037                                                           15    9605
XP_005261036                                                           15    9605
XP_005261035                                                           15    9605
XP_006724068                                                           15    9605
XP_005269061                                                           15    9605
XP_006716667                                                           15    9605
XP_005272371                                                           15    9605
XP_005265846                                                           15    9605
XP_005245374                                                           15    9605
XP_005276018                                                           15    9605
NP_054785                                                              15    9605

>XP_006719294
          Length = 2348

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1103 QAQELGIP 1110




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1187 QAQELGIP 1194




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1211 QAQELGIP 1218




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1283 QAQELGIP 1290




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1355 QAQELGIP 1362




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1379 QAQELGIP 1386




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1403 QAQELGIP 1410




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1415 QAQELGIP 1422




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1463 QAQELGIP 1470




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1487 QAQELGIP 1494




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1511 QAQELGIP 1518




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1535 QAQELGIP 1542




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1583 QAQELGIP 1590




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1595 QAQELGIP 1602




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+Q+L IP
Sbjct: 1235 QAQQLGIP 1242




 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    QSQELAIP 8
            Q QEL IP
Sbjct: 1259 QVQELGIP 1266



>NP_001138947
          Length = 2351

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1103 QAQELGIP 1110




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1187 QAQELGIP 1194




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1211 QAQELGIP 1218




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1283 QAQELGIP 1290




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1355 QAQELGIP 1362




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1379 QAQELGIP 1386




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1403 QAQELGIP 1410




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1415 QAQELGIP 1422




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1463 QAQELGIP 1470




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1487 QAQELGIP 1494




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1511 QAQELGIP 1518




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1535 QAQELGIP 1542




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1583 QAQELGIP 1590




 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+QEL IP
Sbjct: 1595 QAQELGIP 1602




 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QSQELAIP 8
            Q+Q+L IP
Sbjct: 1235 QAQQLGIP 1242




 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1    QSQELAIP 8
            Q QEL IP
Sbjct: 1259 QVQELGIP 1266



>NP_079255
          Length = 1123

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 930 QSQSLPLPA 938



>NP_001106203
          Length = 1125

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 932 QSQSLPLPA 940



>NP_997297
          Length = 1318

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QSQELAIPA 9
           Q+QE A+P+
Sbjct: 929 QAQEAAVPS 937



>XP_005268373
          Length = 984

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 930 QSQSLPLPA 938



>XP_005268372
          Length = 1043

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 989 QSQSLPLPA 997



>XP_005268371
          Length = 1067

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 936 QSQSLPLPA 944



>XP_006720031
          Length = 1080

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 862 QSQSLPLPA 870



>XP_005268370
          Length = 1112

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 894 QSQSLPLPA 902



>XP_006720030
          Length = 1128

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 910 QSQSLPLPA 918



>XP_005268369
          Length = 1132

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 914 QSQSLPLPA 922



>XP_006720029
          Length = 1150

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 932 QSQSLPLPA 940



>XP_005268367
          Length = 1154

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 936 QSQSLPLPA 944



>XP_005268366
          Length = 1180

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 962 QSQSLPLPA 970



>XP_005268365
          Length = 1182

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 989 QSQSLPLPA 997



>XP_005268364
          Length = 1207

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSQELAIPA 9
           QSQ L +PA
Sbjct: 989 QSQSLPLPA 997



>XP_006715752
          Length = 1225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QSQELAIP 8
           QSQE+A P
Sbjct: 909 QSQEIARP 916



>NP_001243084
          Length = 2290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QSQELAIP 8
           QS+ LA+P
Sbjct: 209 QSETLAVP 216



>NP_963889
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QSQELAIPA 9
          Q+Q++ IPA
Sbjct: 62 QTQQVLIPA 70



>NP_056943
          Length = 376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QSQELAIPA 9
           Q+Q++ IPA
Sbjct: 101 QTQQVLIPA 109



>NP_001273575
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QSQELAIP 8
          Q QEL++P
Sbjct: 14 QVQELSVP 21



>NP_001138397
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>NP_001269849
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>NP_001171517
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>NP_002453
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>NP_001139373
          Length = 520

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQELAIPA 9
           +QEL +PA
Sbjct: 395 TQELGLPA 402



>NP_060297
          Length = 518

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SQELAIPA 9
           +QEL +PA
Sbjct: 393 TQELGLPA 400



>NP_001265869
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QSQELAIPA 9
          Q+Q++ IPA
Sbjct: 51 QTQQVLIPA 59



>NP_001274166
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 112 QLQELCIP 119



>NP_060840
          Length = 238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QELAIP 8
           QELA+P
Sbjct: 114 QELAVP 119



>NP_002454
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QELAIPA 9
           Q+LA+PA
Sbjct: 114 QDLALPA 120



>NP_001017924
          Length = 550

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ELAIPA 9
           ELAIPA
Sbjct: 311 ELAIPA 316



>NP_001271152
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ELAIPA 9
           ELAIPA
Sbjct: 321 ELAIPA 326



>NP_001074472
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 112 QLQELCIP 119



>NP_001074473
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 112 QLQELCIP 119



>XP_005276308
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 136 QLQELCIP 143



>XP_006724069
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QELAIPA 9
           Q+LA+PA
Sbjct: 114 QDLALPA 120



>XP_005261041
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QELAIPA 9
           Q+LA+PA
Sbjct: 114 QDLALPA 120



>XP_005261040
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QELAIPA 9
           Q+LA+PA
Sbjct: 114 QDLALPA 120



>XP_005261039
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>XP_005261038
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>XP_005261037
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>XP_005261036
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>XP_005261035
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 66 QDLALPA 72



>XP_006724068
          Length = 685

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  QELAIPA 9
          Q+LA+PA
Sbjct: 89 QDLALPA 95



>XP_005269061
          Length = 207

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   QELAIP 8
           QELA+P
Sbjct: 114 QELAVP 119



>XP_006716667
          Length = 768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QSQELAIP 8
          Q QEL++P
Sbjct: 14 QVQELSVP 21



>XP_005272371
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 112 QLQELCIP 119



>XP_005265846
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 136 QLQELCIP 143



>XP_005245374
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2 SQELAIPA 9
         SQE A+PA
Sbjct: 2 SQEGAVPA 9



>XP_005276018
          Length = 248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 136 QLQELCIP 143



>NP_054785
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSQELAIP 8
           Q QEL IP
Sbjct: 112 QLQELCIP 119



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.116    0.286 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 525,898
Number of extensions: 811
Number of successful extensions: 83
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 83
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)