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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              18    1135
NP_065107                                                              17    2528
NP_689481                                                              17    2528
XP_006719055                                                           17    2528
XP_005253773                                                           17    2528
NP_001152759                                                           16    5631
NP_000356                                                              16    5631
NP_055811                                                              16    5631
NP_001139806                                                           16    5631
XP_006715737                                                           16    5631
XP_005247296                                                           16    5631
XP_006713604                                                           16    5631
XP_005247295                                                           16    5631
NP_001124432                                                           16    5631
NP_005227                                                              16    7355
NP_006725                                                              16    7355
NP_060009                                                              16    7355
XP_006724347                                                           16    7355
XP_006724346                                                           16    7355
XP_006721125                                                           16    7355
XP_005267014                                                           16    7355
NP_038464                                                              15    9605
NP_995314                                                              15    9605
NP_078904                                                              15    9605
NP_001182412                                                           15    9605
XP_006712264                                                           15    9605
XP_006712263                                                           15    9605

>NP_003290
          Length = 803

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QSISELIGQ 9
           QS SELIGQ
Sbjct: 186 QSTSELIGQ 194



>NP_065107
          Length = 552

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SISELIGQ 9
           SISELIG+
Sbjct: 151 SISELIGE 158



>NP_689481
          Length = 475

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   QSISELIGQ 9
           Q+ SELIGQ
Sbjct: 384 QAASELIGQ 392



>XP_006719055
          Length = 419

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SISELIGQ 9
           SISELIG+
Sbjct: 151 SISELIGE 158



>XP_005253773
          Length = 509

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SISELIGQ 9
           SISELIG+
Sbjct: 151 SISELIGE 158



>NP_001152759
          Length = 286

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QSISELIG 8
          QS+ ELIG
Sbjct: 57 QSLGELIG 64



>NP_000356
          Length = 249

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QSISELIG 8
          QS+ ELIG
Sbjct: 20 QSLGELIG 27



>NP_055811
          Length = 1655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    SISELIGQ 9
            SIS LIGQ
Sbjct: 1157 SISALIGQ 1164



>NP_001139806
          Length = 1562

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QSISELIGQ 9
           + ISE++GQ
Sbjct: 530 EGISEILGQ 538



>XP_006715737
          Length = 1349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QSISELIGQ 9
           + ISE++GQ
Sbjct: 530 EGISEILGQ 538



>XP_005247296
          Length = 1673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    SISELIGQ 9
            SIS LIGQ
Sbjct: 1175 SISALIGQ 1182



>XP_006713604
          Length = 1685

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    SISELIGQ 9
            SIS LIGQ
Sbjct: 1187 SISALIGQ 1194



>XP_005247295
          Length = 1705

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    SISELIGQ 9
            SIS LIGQ
Sbjct: 1207 SISALIGQ 1214



>NP_001124432
          Length = 1693

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    SISELIGQ 9
            SIS LIGQ
Sbjct: 1195 SISALIGQ 1202



>NP_005227
          Length = 916

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +SIS+LIG
Sbjct: 727 KSISDLIG 734



>NP_006725
          Length = 2446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SISELIGQ 9
            SIS+++GQ
Sbjct: 1352 SISQILGQ 1359



>NP_060009
          Length = 4116

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QSISELIG 8
           Q ISE++G
Sbjct: 958 QQISEIVG 965



>XP_006724347
          Length = 1002

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ISELIGQ 9
           +SEL+GQ
Sbjct: 544 VSELLGQ 550



>XP_006724346
          Length = 1003

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ISELIGQ 9
           +SEL+GQ
Sbjct: 544 VSELLGQ 550



>XP_006721125
          Length = 4070

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QSISELIG 8
           Q ISE++G
Sbjct: 912 QQISEIVG 919



>XP_005267014
          Length = 2446

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SISELIGQ 9
            SIS+++GQ
Sbjct: 1352 SISQILGQ 1359



>NP_038464
          Length = 1128

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +S+SEL+G
Sbjct: 837 RSLSELLG 844



>NP_995314
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +S+SEL+G
Sbjct: 843 RSLSELLG 850



>NP_078904
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +SIS+L+G
Sbjct: 143 ESISDLLG 150



>NP_001182412
          Length = 714

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +SIS+L+G
Sbjct: 143 ESISDLLG 150



>XP_006712264
          Length = 1126

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +S+SEL+G
Sbjct: 835 RSLSELLG 842



>XP_006712263
          Length = 1132

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSISELIG 8
           +S+SEL+G
Sbjct: 841 RSLSELLG 848



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.127    0.295 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 373,233
Number of extensions: 537
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)