Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_037493                                                              16    5631
XP_006723429                                                           16    5631
XP_006723428                                                           16    5631
NP_001001557                                                           16    7355
XP_005255279                                                           16    7355
NP_001073886                                                           16    7355
NP_001159521                                                           15    9605
NP_060109                                                              15    9605
NP_001364                                                              15    9605
NP_060289                                                              15    9605
NP_001273144                                                           15    9605
NP_116237                                                              15    9605
NP_659441                                                              15    9605
NP_085150                                                              15    9605
XP_006725181                                                           15    9605
XP_006725180                                                           15    9605
XP_005258694                                                           15    9605
XP_006722987                                                           15    9605
XP_005254029                                                           15    9605
XP_006716362                                                           15    9605
XP_006716361                                                           15    9605
XP_006711798                                                           15    9605
XP_005245194                                                           15    9605
NP_001131141                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    QSFVDRSL 8
            QSFVDRSL
Sbjct: 1817 QSFVDRSL 1824
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    QSFVDRSL 8
            QSFVDRSL
Sbjct: 1817 QSFVDRSL 1824
>NP_037493
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSFVDRSLF 9
           QSF D S+F
Sbjct: 155 QSFSDMSIF 163
>XP_006723429
          Length = 397

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  QSFVDRSLF 9
          QSF D S+F
Sbjct: 70 QSFSDMSIF 78
>XP_006723428
          Length = 482

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QSFVDRSLF 9
           QSF D S+F
Sbjct: 155 QSFSDMSIF 163
>NP_001001557
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SFVDRSL 8
           SFVDR L
Sbjct: 129 SFVDRGL 135
>XP_005255279
          Length = 855

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSFVDRSL 8
           +SF+DRS+
Sbjct: 655 ESFLDRSV 662
>NP_001073886
          Length = 869

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QSFVDRSL 8
           +SF+DRS+
Sbjct: 669 ESFLDRSV 676
>NP_001159521
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSFVDRSL 8
           Q FV RSL
Sbjct: 229 QDFVSRSL 236
>NP_060109
          Length = 2916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QSFVDRSLF 9
           Q+FV +SLF
Sbjct: 775 QAFVLKSLF 783
>NP_001364
          Length = 4515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FVDRSLF 9
            F D+SLF
Sbjct: 2645 FTDKSLF 2651
>NP_060289
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SFVDRSL 8
           SFVD+S+
Sbjct: 311 SFVDKSM 317
>NP_001273144
          Length = 293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SFVDRSLF 9
          S+VD +LF
Sbjct: 56 SYVDETLF 63
>NP_116237
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SFVDRSLF 9
          S+VD +LF
Sbjct: 32 SYVDETLF 39
>NP_659441
          Length = 787

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FVDRSL 8
           F+DRSL
Sbjct: 156 FIDRSL 161
>NP_085150
          Length = 941

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QSFVDRSLF 9
           Q F D+SL+
Sbjct: 592 QPFADQSLY 600
>XP_006725181
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FVDRSL 8
           F+DRSL
Sbjct: 156 FIDRSL 161
>XP_006725180
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FVDRSL 8
           F+DRSL
Sbjct: 156 FIDRSL 161
>XP_005258694
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QSFVDRSL 8
           Q FV RSL
Sbjct: 229 QDFVSRSL 236
>XP_006722987
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   SFVDRS 7
           SF+DRS
Sbjct: 316 SFIDRS 321
>XP_005254029
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  SFVDRSLF 9
          S+VD +LF
Sbjct: 32 SYVDETLF 39
>XP_006716362
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FVDRSL 8
           F+DRSL
Sbjct: 156 FIDRSL 161
>XP_006716361
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   FVDRSL 8
           F+DRSL
Sbjct: 156 FIDRSL 161
>XP_006711798
          Length = 4586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    FVDRSLF 9
            F D+SLF
Sbjct: 2651 FTDKSLF 2657
>XP_005245194
          Length = 1111

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QSFVDRSLF 9
           + ++DRS+F
Sbjct: 988 EGYLDRSVF 996
>NP_001131141
          Length = 1076

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QSFVDRSLF 9
           + ++DRS+F
Sbjct: 953 EGYLDRSVF 961
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.143    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,025
Number of extensions: 712
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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