Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115927                                                              20     390
NP_056019                                                              17    4312
XP_006725002                                                           17    4312
XP_006715725                                                           17    4312
XP_006715724                                                           17    4312
XP_006715723                                                           17    4312
XP_006715722                                                           17    4312
XP_005276117                                                           17    4312
NP_775771                                                              16    5631
NP_003102                                                              16    5631
NP_001017371                                                           16    5631
NP_001166183                                                           16    5631
XP_006720466                                                           16    5631
XP_005254230                                                           16    5631
XP_006720465                                                           16    5631
XP_005254228                                                           16    5631
NP_005974                                                              16    7355
NP_001230049                                                           16    7355
NP_055325                                                              16    7355
NP_149015                                                              16    7355
XP_006715989                                                           16    7355
XP_006714550                                                           16    7355
NP_001804                                                              15    9605
NP_653223                                                              15    9605
NP_004527                                                              15    9605
NP_001273663                                                           15    9605
NP_075043                                                              15    9605
NP_001186978                                                           15    9605
NP_443111                                                              15    9605
NP_115830                                                              15    9605
NP_004188                                                              15    9605
NP_954658                                                              15    9605
XP_006725522                                                           15    9605
XP_006725520                                                           15    9605
XP_006725519                                                           15    9605
XP_006725518                                                           15    9605
XP_006725517                                                           15    9605
XP_006725516                                                           15    9605
XP_005276859                                                           15    9605
XP_005276857                                                           15    9605
XP_006726285                                                           15    9605
XP_006726284                                                           15    9605
XP_006726283                                                           15    9605
XP_006726282                                                           15    9605
XP_006719279                                                           15    9605
XP_005268670                                                           15    9605
XP_006719278                                                           15    9605
XP_005248581                                                           15    9605
XP_005248580                                                           15    9605
XP_006714692                                                           15    9605
XP_006714691                                                           15    9605
XP_006714690                                                           15    9605
XP_006714689                                                           15    9605
XP_006714629                                                           15    9605
XP_005271973                                                           15    9605
NP_001164275                                                           15    9605
NP_001164279                                                           15    9605
>NP_115927
          Length = 1321

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QRQVFSVVP 9
           QRQVFSV P
Sbjct: 660 QRQVFSVAP 668
>NP_056019
          Length = 1657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QRQVFSVVP 9
            Q QV+ VVP
Sbjct: 1081 QAQVYEVVP 1089
>XP_006725002
          Length = 599

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QRQVFSVVP 9
           ++QVF++VP
Sbjct: 248 EQQVFTLVP 256
>XP_006715725
          Length = 1516

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QRQVFSVVP 9
            Q QV+ VVP
Sbjct: 1081 QAQVYEVVP 1089
>XP_006715724
          Length = 1533

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QRQVFSVVP 9
            Q QV+ VVP
Sbjct: 1081 QAQVYEVVP 1089
>XP_006715723
          Length = 1559

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QRQVFSVVP 9
            Q QV+ VVP
Sbjct: 1081 QAQVYEVVP 1089
>XP_006715722
          Length = 1614

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QRQVFSVVP 9
            Q QV+ VVP
Sbjct: 1081 QAQVYEVVP 1089
>XP_005276117
          Length = 599

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QRQVFSVVP 9
           ++QVF++VP
Sbjct: 248 EQQVFTLVP 256
>NP_775771
          Length = 1244

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           Q FSVVP
Sbjct: 829 QTFSVVP 835
>NP_003102
          Length = 781

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQVFSVVP 9
           +Q+FSV P
Sbjct: 149 QQIFSVAP 156
>NP_001017371
          Length = 713

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RQVFSVVP 9
          +Q+FSV P
Sbjct: 81 QQIFSVAP 88
>NP_001166183
          Length = 778

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQVFSVVP 9
           +Q+FSV P
Sbjct: 146 QQIFSVAP 153
>XP_006720466
          Length = 1175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           Q FSVVP
Sbjct: 760 QTFSVVP 766
>XP_005254230
          Length = 1175

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           Q FSVVP
Sbjct: 760 QTFSVVP 766
>XP_006720465
          Length = 1239

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           Q FSVVP
Sbjct: 824 QTFSVVP 830
>XP_005254228
          Length = 1250

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           Q FSVVP
Sbjct: 835 QTFSVVP 841
>NP_005974
          Length = 424

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           QVF +VP
Sbjct: 366 QVFGIVP 372
>NP_001230049
          Length = 210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           QVF +VP
Sbjct: 152 QVFGIVP 158
>NP_055325
          Length = 4935

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSVVP
Sbjct: 2619 VFSVVP 2624
>NP_149015
          Length = 5142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSVVP
Sbjct: 2619 VFSVVP 2624
>XP_006715989
          Length = 5148

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSVVP
Sbjct: 2619 VFSVVP 2624
>XP_006714550
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           QVF +VP
Sbjct: 321 QVFGIVP 327
>NP_001804
          Length = 2701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    QRQVFSVV 8
            QR++FS++
Sbjct: 1042 QRKIFSLI 1049
>NP_653223
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   QVFSVVP 9
           QVF V+P
Sbjct: 249 QVFCVIP 255
>NP_004527
          Length = 1403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>NP_001273663
          Length = 2580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    QRQVFSVV 8
            QR++FS++
Sbjct: 1017 QRKIFSLI 1024
>NP_075043
          Length = 1241

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 955 VFSVIP 960
>NP_001186978
          Length = 2715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RQVFSVV 8
           RQ+FSV+
Sbjct: 214 RQIFSVM 220
>NP_443111
          Length = 724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 316 QYSVYSIVP 324
>NP_115830
          Length = 368

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QVFSVVP 9
           QV+S+VP
Sbjct: 174 QVYSLVP 180
>NP_004188
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QVFSVVP 9
           QV+S+VP
Sbjct: 174 QVYSLVP 180
>NP_954658
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 315 QYSVYSIVP 323
>XP_006725522
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 479 VFSVIP 484
>XP_006725520
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 729 VFSVIP 734
>XP_006725519
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 853 VFSVIP 858
>XP_006725518
          Length = 1219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 933 VFSVIP 938
>XP_006725517
          Length = 1348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>XP_006725516
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>XP_005276859
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 479 VFSVIP 484
>XP_005276857
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 729 VFSVIP 734
>XP_006726285
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 853 VFSVIP 858
>XP_006726284
          Length = 1219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 933 VFSVIP 938
>XP_006726283
          Length = 1348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>XP_006726282
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>XP_006719279
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 316 QYSVYSIVP 324
>XP_005268670
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 246 QYSVYSIVP 254
>XP_006719278
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 316 QYSVYSIVP 324
>XP_005248581
          Length = 765

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 479 VFSVIP 484
>XP_005248580
          Length = 1015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 729 VFSVIP 734
>XP_006714692
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 853 VFSVIP 858
>XP_006714691
          Length = 1219

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VFSVVP 9
           VFSV+P
Sbjct: 933 VFSVIP 938
>XP_006714690
          Length = 1348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>XP_006714689
          Length = 1352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    VFSVVP 9
            VFSV+P
Sbjct: 1117 VFSVIP 1122
>XP_006714629
          Length = 409

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q+Q+FS  P
Sbjct: 155 QKQIFSPPP 163
>XP_005271973
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q+Q+FS  P
Sbjct: 158 QKQIFSPPP 166
>NP_001164275
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 316 QYSVYSIVP 324
>NP_001164279
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QRQVFSVVP 9
           Q  V+S+VP
Sbjct: 316 QYSVYSIVP 324
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.137    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 471,171
Number of extensions: 755
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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