Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006715                                                              18    1482
NP_005913                                                              18    1482
XP_005267046                                                           18    1482
XP_005267045                                                           18    1482
NP_001185863                                                           17    4312
NP_258261                                                              17    4312
XP_005249527                                                           17    4312
XP_006715303                                                           17    4312
NP_000171                                                              16    7355
NP_000119                                                              16    7355
NP_079106                                                              16    7355
XP_006726609                                                           16    7355
XP_006719787                                                           16    7355
XP_005262880                                                           16    7355
XP_005262879                                                           16    7355
XP_006714200                                                           16    7355
XP_005262878                                                           16    7355
XP_006710027                                                           16    7355
>NP_006715
          Length = 1558

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QRARIALV 8
           QRARIALV
Sbjct: 555 QRARIALV 562
>NP_005913
          Length = 1608

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QRARIALV 8
           QRARIALV
Sbjct: 555 QRARIALV 562
>XP_005267046
          Length = 1554

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QRARIALV 8
           QRARIALV
Sbjct: 555 QRARIALV 562
>XP_005267045
          Length = 1604

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QRARIALV 8
           QRARIALV
Sbjct: 555 QRARIALV 562
>NP_001185863
          Length = 1492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   QRARIAL 7
           QRARIAL
Sbjct: 730 QRARIAL 736
>NP_258261
          Length = 1464

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   QRARIAL 7
           QRARIAL
Sbjct: 702 QRARIAL 708
>XP_005249527
          Length = 822

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   QRARIAL 7
           QRARIAL
Sbjct: 730 QRARIAL 736
>XP_006715303
          Length = 1463

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   QRARIAL 7
           QRARIAL
Sbjct: 730 QRARIAL 736
>NP_000171
          Length = 1103

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   ARIALVT 9
           AR+ALVT
Sbjct: 182 ARVALVT 188
>NP_000119
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QRARIALVT 9
           Q+A+I LVT
Sbjct: 533 QKAKIPLVT 541
>NP_079106
          Length = 534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1  QRARIALVT 9
          Q+A++AL+T
Sbjct: 42 QKAQVALMT 50
>XP_006726609
          Length = 2838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RARIALVT 9
            R R+ALVT
Sbjct: 2452 RCRLALVT 2459
>XP_006719787
          Length = 2838

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RARIALVT 9
            R R+ALVT
Sbjct: 2452 RCRLALVT 2459
>XP_005262880
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QRARIALVT 9
           Q+A+I LVT
Sbjct: 444 QKAKIPLVT 452
>XP_005262879
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QRARIALVT 9
           Q+A+I LVT
Sbjct: 502 QKAKIPLVT 510
>XP_006714200
          Length = 610

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QRARIALVT 9
           Q+A+I LVT
Sbjct: 518 QKAKIPLVT 526
>XP_005262878
          Length = 626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QRARIALVT 9
           Q+A+I LVT
Sbjct: 534 QKAKIPLVT 542
>XP_006710027
          Length = 2837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    RARIALVT 9
            R R+ALVT
Sbjct: 2451 RCRLALVT 2458
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.136    0.328 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 245,239
Number of extensions: 281
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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