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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_115803                                                              18    1482
XP_005261689                                                           16    5631
XP_006724326                                                           16    5631
NP_001275911                                                           15    9605
NP_775800                                                              15    9605
NP_001275910                                                           15    9605
NP_001275909                                                           15    9605
XP_005258260                                                           15    9605
XP_005258259                                                           15    9605
XP_005258258                                                           15    9605

>NP_115803
          Length = 1156

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QQSSISAQ 8
           QQSSISAQ
Sbjct: 620 QQSSISAQ 627




 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   QQSSISAQ 8
           QQSSISAQ
Sbjct: 632 QQSSISAQ 639



>XP_005261689
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  QSSISAQL 9
          Q+S+SAQL
Sbjct: 75 QASVSAQL 82



>XP_006724326
          Length = 717

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  QSSISAQL 9
          Q+S+SAQL
Sbjct: 75 QASVSAQL 82



>NP_001275911
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



>NP_775800
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



>NP_001275910
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



>NP_001275909
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



>XP_005258260
          Length = 353

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



>XP_005258259
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



>XP_005258258
          Length = 533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  QQSSISAQL 9
          Q+SS+SA L
Sbjct: 13 QESSVSALL 21



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.100    0.204 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 206,462
Number of extensions: 171
Number of successful extensions: 11
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)