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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              21     175
XP_005271924                                                           21     175
XP_005271923                                                           21     175
NP_001191676                                                           17    3301
NP_002904                                                              17    3301
NP_002462                                                              16    5631
NP_000248                                                              16    5631
XP_005267753                                                           16    5631
XP_006720215                                                           16    5631
XP_006720214                                                           16    5631
XP_005267751                                                           16    5631
NP_001005514                                                           16    5631
NP_001264198                                                           16    7355
NP_036553                                                              16    7355
NP_112211                                                              16    7355
NP_056155                                                              16    7355
XP_005267209                                                           16    7355
XP_005265063                                                           16    7355
NP_001005482                                                           16    7355
NP_689491                                                              15    9605
NP_001952                                                              15    9605
NP_115956                                                              15    9605
NP_001239004                                                           15    9605
NP_001239002                                                           15    9605
NP_004776                                                              15    9605
NP_001123484                                                           15    9605
NP_001239005                                                           15    9605
XP_005255760                                                           15    9605
XP_006721044                                                           15    9605
XP_005255759                                                           15    9605
NP_001004759                                                           15    9605

>NP_001261
          Length = 1710

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QQNVRGMRK 9
           QQNVRGM+K
Sbjct: 340 QQNVRGMKK 348



>XP_005271924
          Length = 1710

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QQNVRGMRK 9
           QQNVRGM+K
Sbjct: 340 QQNVRGMKK 348



>XP_005271923
          Length = 1798

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QQNVRGMRK 9
           QQNVRGM+K
Sbjct: 340 QQNVRGMKK 348



>NP_001191676
          Length = 1148

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QQNVRGMRK 9
           Q+NV+G RK
Sbjct: 508 QKNVQGKRK 516



>NP_002904
          Length = 1147

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QQNVRGMRK 9
           Q+NV+G RK
Sbjct: 508 QKNVQGKRK 516



>NP_002462
          Length = 1939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    QNVRGMRK 9
            ++V+GMRK
Sbjct: 1837 ESVKGMRK 1844



>NP_000248
          Length = 1935

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    QNVRGMRK 9
            ++V+GMRK
Sbjct: 1835 ESVKGMRK 1842



>XP_005267753
          Length = 1935

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    QNVRGMRK 9
            ++V+GMRK
Sbjct: 1835 ESVKGMRK 1842



>XP_006720215
          Length = 1968

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    QNVRGMRK 9
            ++V+GMRK
Sbjct: 1866 ESVKGMRK 1873



>XP_006720214
          Length = 1978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    QNVRGMRK 9
            ++V+GMRK
Sbjct: 1876 ESVKGMRK 1883



>XP_005267751
          Length = 1979

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    QNVRGMRK 9
            ++V+GMRK
Sbjct: 1877 ESVKGMRK 1884



>NP_001005514
          Length = 310

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QQNVRGMRK 9
           +++V+GMRK
Sbjct: 228 KKSVKGMRK 236



>NP_001264198
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  QNVRGMR 8
          +NV+GMR
Sbjct: 47 RNVKGMR 53



>NP_036553
          Length = 1914

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QNVRGMRK 9
           +N RGM+K
Sbjct: 966 KNARGMQK 973



>NP_112211
          Length = 165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VRGMRK 9
           +RGMRK
Sbjct: 151 IRGMRK 156



>NP_056155
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QQNVRGMRK 9
           Q N++ MRK
Sbjct: 151 QSNIKHMRK 159



>XP_005267209
          Length = 108

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  VRGMRK 9
          +RGMRK
Sbjct: 94 IRGMRK 99



>XP_005265063
          Length = 903

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QQNVRGMRK 9
           Q N++ MRK
Sbjct: 151 QSNIKHMRK 159



>NP_001005482
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QQNVRGMRK 9
           +++VRG+RK
Sbjct: 233 KKSVRGVRK 241



>NP_689491
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2  QNVRGMRK 9
          + +RG+RK
Sbjct: 74 EEIRGLRK 81



>NP_001952
          Length = 858

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   QQNVRGMR 8
           ++N+RG+R
Sbjct: 694 EENMRGVR 701



>NP_115956
          Length = 430

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QQNVRGMRK 9
           ++N+RG+ K
Sbjct: 110 KENIRGLDK 118




 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QQNVRGMRK 9
           ++N+RG+ K
Sbjct: 158 KENIRGLDK 166



>NP_001239004
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QNVRGMR 8
          QNV G+R
Sbjct: 89 QNVEGLR 95



>NP_001239002
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QNVRGMR 8
          QNV G+R
Sbjct: 91 QNVEGLR 97



>NP_004776
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   QNVRGMR 8
           QNV G+R
Sbjct: 202 QNVEGLR 208



>NP_001123484
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   QNVRGMR 8
           QNV G+R
Sbjct: 202 QNVEGLR 208



>NP_001239005
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QNVRGMR 8
          QNV G+R
Sbjct: 91 QNVEGLR 97



>XP_005255760
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QNVRGMR 8
          QNV G+R
Sbjct: 67 QNVEGLR 73



>XP_006721044
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QNVRGMR 8
          QNV G+R
Sbjct: 67 QNVEGLR 73



>XP_005255759
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   QNVRGMR 8
           QNV G+R
Sbjct: 250 QNVEGLR 256



>NP_001004759
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NVRGMR 8
           NVRG+R
Sbjct: 345 NVRGLR 350



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.129    0.348 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 435,148
Number of extensions: 632
Number of successful extensions: 32
Number of sequences better than 10000.0: 31
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)