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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005350                                                              19     869
NP_008851                                                              18    1935
NP_001189364                                                           18    1935
NP_001159436                                                           18    1935
NP_001159435                                                           18    1935
XP_006726844                                                           16    7355
XP_006723587                                                           16    7355
XP_006710121                                                           16    7355

>NP_005350
          Length = 552

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QQAATAQAS 9
           QQAATAQA+
Sbjct: 449 QQAATAQAA 457



>NP_008851
          Length = 1998

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QQAATAQAS 9
           QQAATA AS
Sbjct: 459 QQAATATAS 467



>NP_001189364
          Length = 2009

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QQAATAQAS 9
           QQAATA AS
Sbjct: 459 QQAATATAS 467



>NP_001159436
          Length = 1981

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QQAATAQAS 9
           QQAATA AS
Sbjct: 459 QQAATATAS 467



>NP_001159435
          Length = 2009

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QQAATAQAS 9
           QQAATA AS
Sbjct: 459 QQAATATAS 467



>XP_006726844
          Length = 225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QQAATAQ 7
           +QAATAQ
Sbjct: 202 EQAATAQ 208



>XP_006723587
          Length = 225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QQAATAQ 7
           +QAATAQ
Sbjct: 202 EQAATAQ 208



>XP_006710121
          Length = 225

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   QQAATAQ 7
           +QAATAQ
Sbjct: 202 EQAATAQ 208



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.284   0.0825    0.172 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 147,965
Number of extensions: 111
Number of successful extensions: 8
Number of sequences better than 10000.0: 8
Number of HSP's gapped: 8
Number of HSP's successfully gapped: 8
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)