Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057235                                                              16    5631
NP_056044                                                              16    7355
NP_001073944                                                           16    7355
NP_003893                                                              16    7355
NP_001127949                                                           16    7355
NP_002573                                                              16    7355
NP_001229921                                                           16    7355
XP_005256625                                                           16    7355
XP_005256624                                                           16    7355
XP_005256622                                                           16    7355
XP_006721555                                                           16    7355
XP_006721554                                                           16    7355
XP_005246602                                                           16    7355
XP_006712584                                                           16    7355
XP_005246601                                                           16    7355
XP_005246600                                                           16    7355
XP_006711085                                                           16    7355
XP_006711084                                                           16    7355
XP_005271366                                                           16    7355
NP_003679                                                              15    9605
NP_001119526                                                           15    9605
NP_061978                                                              15    9605
NP_001119527                                                           15    9605
NP_004111                                                              15    9605
NP_001273279                                                           15    9605
NP_444507                                                              15    9605
NP_064579                                                              15    9605
NP_999627                                                              15    9605
NP_001263997                                                           15    9605
NP_002044                                                              15    9605
NP_001263992                                                           15    9605
NP_001263995                                                           15    9605
NP_001263991                                                           15    9605
NP_001263993                                                           15    9605
NP_001263994                                                           15    9605
NP_001263990                                                           15    9605
NP_060754                                                              15    9605
NP_001244299                                                           15    9605
XP_006724629                                                           15    9605
XP_006724628                                                           15    9605
XP_005272743                                                           15    9605
XP_006724088                                                           15    9605
XP_005255219                                                           15    9605
XP_006712702                                                           15    9605
XP_005246745                                                           15    9605
XP_005246744                                                           15    9605
XP_005246743                                                           15    9605
XP_005246742                                                           15    9605
XP_006712701                                                           15    9605
XP_005246741                                                           15    9605
XP_006712700                                                           15    9605
XP_005246740                                                           15    9605
XP_005246739                                                           15    9605
XP_005246738                                                           15    9605
XP_005270798                                                           15    9605
XP_006710637                                                           15    9605
>NP_057235
          Length = 1235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QPEALKVAA 9
           QP++LKV A
Sbjct: 739 QPKSLKVRA 747
>NP_056044
          Length = 1309

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    PEALKVA 8
            P+ALKVA
Sbjct: 1140 PKALKVA 1146
>NP_001073944
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EALKVAA 9
           EALK+AA
Sbjct: 449 EALKIAA 455
>NP_003893
          Length = 644

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QPEALKVA 8
          QP+A KVA
Sbjct: 72 QPDAKKVA 79
>NP_001127949
          Length = 578

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QPE +K+A
Sbjct: 426 QPEQVKIA 433
>NP_002573
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QPE +K+A
Sbjct: 487 QPEQVKIA 494
>NP_001229921
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QPE +K+A
Sbjct: 441 QPEQVKIA 448
>XP_005256625
          Length = 1347

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    PEALKVA 8
            P+ALKVA
Sbjct: 1178 PKALKVA 1184
>XP_005256624
          Length = 1348

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    PEALKVA 8
            P+ALKVA
Sbjct: 1179 PKALKVA 1185
>XP_005256622
          Length = 1383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    PEALKVA 8
            P+ALKVA
Sbjct: 1214 PKALKVA 1220
>XP_006721555
          Length = 1444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    PEALKVA 8
            P+ALKVA
Sbjct: 1275 PKALKVA 1281
>XP_006721554
          Length = 1445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    PEALKVA 8
            P+ALKVA
Sbjct: 1276 PKALKVA 1282
>XP_005246602
          Length = 651

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EALKVAA 9
           EALK+AA
Sbjct: 449 EALKIAA 455
>XP_006712584
          Length = 657

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EALKVAA 9
           EALK+AA
Sbjct: 449 EALKIAA 455
>XP_005246601
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EALKVAA 9
           EALK+AA
Sbjct: 449 EALKIAA 455
>XP_005246600
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EALKVAA 9
           EALK+AA
Sbjct: 449 EALKIAA 455
>XP_006711085
          Length = 643

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QPEALKVA 8
          QP+A KVA
Sbjct: 72 QPDAKKVA 79
>XP_006711084
          Length = 664

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QP+A KVA
Sbjct: 93  QPDAKKVA 100
>XP_005271366
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QP+A KVA
Sbjct: 93  QPDAKKVA 100
>NP_003679
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QPEALKV 7
           +P+ALKV
Sbjct: 833 EPQALKV 839
>NP_001119526
          Length = 898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QPEALKV 7
           +P+ALKV
Sbjct: 810 EPQALKV 816
>NP_061978
          Length = 4242

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QPEALKVAA 9
            QP A+ VAA
Sbjct: 3487 QPRAVPVAA 3495
>NP_001119527
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QPEALKV 7
           +P+ALKV
Sbjct: 809 EPQALKV 815
>NP_004111
          Length = 591

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  PEALKV 7
          PEALK+
Sbjct: 25 PEALKI 30
>NP_001273279
          Length = 604

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QPEALKV 7
           QP ALKV
Sbjct: 594 QPTALKV 600
>NP_444507
          Length = 686

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QPEALKVAA 9
           Q EA++VAA
Sbjct: 307 QAEAIEVAA 315
>NP_064579
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>NP_999627
          Length = 774

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QPEALKV 7
           QP ALKV
Sbjct: 764 QPTALKV 770
>NP_001263997
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>NP_002044
          Length = 592

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  PEALKV 7
          PEALK+
Sbjct: 25 PEALKI 30
>NP_001263992
          Length = 243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>NP_001263995
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>NP_001263991
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>NP_001263993
          Length = 238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>NP_001263994
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>NP_001263990
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>NP_060754
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  PEALKV 7
          PEALK+
Sbjct: 25 PEALKI 30
>NP_001244299
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QPE+ KVA
Sbjct: 201 QPESPKVA 208
>XP_006724629
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QPEALKV 7
           +P+ALKV
Sbjct: 797 EPQALKV 803
>XP_006724628
          Length = 908

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QPEALKV 7
           +P+ALKV
Sbjct: 820 EPQALKV 826
>XP_005272743
          Length = 920

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   QPEALKV 7
           +P+ALKV
Sbjct: 832 EPQALKV 838
>XP_006724088
          Length = 646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QPEALKVAA 9
           Q EA++VAA
Sbjct: 267 QAEAIEVAA 275
>XP_005255219
          Length = 351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QPEALKVA 8
           QPE+ KVA
Sbjct: 173 QPESPKVA 180
>XP_006712702
          Length = 238

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>XP_005246745
          Length = 244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_005246744
          Length = 264

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_005246743
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_005246742
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_006712701
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>XP_005246741
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_006712700
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>XP_005246740
          Length = 316

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 24 PEKLKVA 30
>XP_005246739
          Length = 317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_005246738
          Length = 322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  PEALKVA 8
          PE LKVA
Sbjct: 50 PEKLKVA 56
>XP_005270798
          Length = 544

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  PEALKV 7
          PEALK+
Sbjct: 25 PEALKI 30
>XP_006710637
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  PEALKV 7
          PEALK+
Sbjct: 25 PEALKI 30
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.116    0.291 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 640,810
Number of extensions: 839
Number of successful extensions: 56
Number of sequences better than 10000.0: 56
Number of HSP's gapped: 56
Number of HSP's successfully gapped: 56
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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