Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001261                                                              21     229
XP_005271924                                                           21     229
XP_005271923                                                           21     229
NP_112211                                                              17    2528
XP_005267209                                                           17    2528
NP_002462                                                              16    5631
NP_000248                                                              16    5631
NP_001036037                                                           16    5631
NP_001262                                                              16    5631
XP_005267753                                                           16    5631
XP_006720215                                                           16    5631
XP_006720214                                                           16    5631
XP_005267751                                                           16    5631
NP_001191676                                                           16    7355
NP_002904                                                              16    7355
NP_115956                                                              16    7355
NP_001264198                                                           16    7355
NP_036553                                                              16    7355
NP_001264262                                                           16    7355
XP_003959998                                                           16    7355
NP_001005514                                                           16    7355
NP_619634                                                              16    7355
NP_821079                                                              15    9605
NP_001120670                                                           15    9605
NP_009198                                                              15    9605
NP_055573                                                              15    9605
NP_689491                                                              15    9605
NP_065816                                                              15    9605
NP_001239004                                                           15    9605
NP_001239002                                                           15    9605
NP_004776                                                              15    9605
NP_001123484                                                           15    9605
NP_001239005                                                           15    9605
XP_005257055                                                           15    9605
XP_005257054                                                           15    9605
XP_005257053                                                           15    9605
XP_005257052                                                           15    9605
XP_005255760                                                           15    9605
XP_006721044                                                           15    9605
XP_005255759                                                           15    9605
XP_005271603                                                           15    9605
XP_005271602                                                           15    9605
XP_006710573                                                           15    9605
XP_006710572                                                           15    9605
XP_006710571                                                           15    9605
XP_006710570                                                           15    9605
XP_006710569                                                           15    9605
XP_006710568                                                           15    9605
XP_006710567                                                           15    9605
XP_006710566                                                           15    9605
XP_005245859                                                           15    9605
NP_001005482                                                           15    9605
NP_001004759                                                           15    9605
>NP_001261
          Length = 1710

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNVRGMRKL 9
           QNVRGM+KL
Sbjct: 341 QNVRGMKKL 349
>XP_005271924
          Length = 1710

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNVRGMRKL 9
           QNVRGM+KL
Sbjct: 341 QNVRGMKKL 349
>XP_005271923
          Length = 1798

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNVRGMRKL 9
           QNVRGM+KL
Sbjct: 341 QNVRGMKKL 349
>NP_112211
          Length = 165

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VRGMRKL 9
           +RGMRKL
Sbjct: 151 IRGMRKL 157
>XP_005267209
          Length = 108

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   VRGMRKL 9
           +RGMRKL
Sbjct: 94  IRGMRKL 100
>NP_002462
          Length = 1939

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QNVRGMRK 8
            ++V+GMRK
Sbjct: 1837 ESVKGMRK 1844
>NP_000248
          Length = 1935

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QNVRGMRK 8
            ++V+GMRK
Sbjct: 1835 ESVKGMRK 1842
>NP_001036037
          Length = 501

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           Q V+G++KL
Sbjct: 330 QKVKGLKKL 338
>NP_001262
          Length = 1828

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           Q V+G++KL
Sbjct: 330 QKVKGLKKL 338
>XP_005267753
          Length = 1935

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QNVRGMRK 8
            ++V+GMRK
Sbjct: 1835 ESVKGMRK 1842
>XP_006720215
          Length = 1968

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QNVRGMRK 8
            ++V+GMRK
Sbjct: 1866 ESVKGMRK 1873
>XP_006720214
          Length = 1978

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QNVRGMRK 8
            ++V+GMRK
Sbjct: 1876 ESVKGMRK 1883
>XP_005267751
          Length = 1979

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    QNVRGMRK 8
            ++V+GMRK
Sbjct: 1877 ESVKGMRK 1884
>NP_001191676
          Length = 1148

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +NV+G RK+
Sbjct: 509 KNVQGKRKI 517
>NP_002904
          Length = 1147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +NV+G RK+
Sbjct: 509 KNVQGKRKI 517
>NP_115956
          Length = 430

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +N+RG+ K+
Sbjct: 111 ENIRGLDKI 119

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +N+RG+ K+
Sbjct: 159 ENIRGLDKI 167
>NP_001264198
          Length = 2925

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  QNVRGMR 7
          +NV+GMR
Sbjct: 47 RNVKGMR 53
>NP_036553
          Length = 1914

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QNVRGMRK 8
           +N RGM+K
Sbjct: 966 KNARGMQK 973
>NP_001264262
          Length = 917

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           QN++G +KL
Sbjct: 473 QNLQGKKKL 481
>XP_003959998
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           QN++G +KL
Sbjct: 195 QNLQGKKKL 203
>NP_001005514
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QNVRGMRK 8
           ++V+GMRK
Sbjct: 229 KSVKGMRK 236
>NP_619634
          Length = 147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VRGMRKL 9
           +RGM+KL
Sbjct: 135 IRGMQKL 141
>NP_821079
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           +  RG+RKL
Sbjct: 151 EQFRGLRKL 159
>NP_001120670
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           Q VRG RK+
Sbjct: 741 QVVRGQRKV 749
>NP_009198
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           Q VRG RK+
Sbjct: 741 QVVRGQRKV 749
>NP_055573
          Length = 992

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +NV+G +KL
Sbjct: 604 KNVKGPKKL 612
>NP_689491
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  QNVRGMRK 8
          + +RG+RK
Sbjct: 74 EEIRGLRK 81
>NP_065816
          Length = 5183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3236 HVRGIKKL 3243
>NP_001239004
          Length = 224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QNVRGMR 7
          QNV G+R
Sbjct: 89 QNVEGLR 95
>NP_001239002
          Length = 226

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QNVRGMR 7
          QNV G+R
Sbjct: 91 QNVEGLR 97
>NP_004776
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QNVRGMR 7
           QNV G+R
Sbjct: 202 QNVEGLR 208
>NP_001123484
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QNVRGMR 7
           QNV G+R
Sbjct: 202 QNVEGLR 208
>NP_001239005
          Length = 215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QNVRGMR 7
          QNV G+R
Sbjct: 91 QNVEGLR 97
>XP_005257055
          Length = 578

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           Q VRG RK+
Sbjct: 514 QVVRGQRKV 522
>XP_005257054
          Length = 745

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           Q VRG RK+
Sbjct: 681 QVVRGQRKV 689
>XP_005257053
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           Q VRG RK+
Sbjct: 741 QVVRGQRKV 749
>XP_005257052
          Length = 805

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QNVRGMRKL 9
           Q VRG RK+
Sbjct: 741 QVVRGQRKV 749
>XP_005255760
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QNVRGMR 7
          QNV G+R
Sbjct: 67 QNVEGLR 73
>XP_006721044
          Length = 202

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  QNVRGMR 7
          QNV G+R
Sbjct: 67 QNVEGLR 73
>XP_005255759
          Length = 342

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   QNVRGMR 7
           QNV G+R
Sbjct: 250 QNVEGLR 256
>XP_005271603
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +NV+G +KL
Sbjct: 604 KNVKGPKKL 612
>XP_005271602
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QNVRGMRKL 9
           +NV+G +KL
Sbjct: 604 KNVKGPKKL 612
>XP_006710573
          Length = 5177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3230 HVRGIKKL 3237
>XP_006710572
          Length = 5188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3241 HVRGIKKL 3248
>XP_006710571
          Length = 5194

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3247 HVRGIKKL 3254
>XP_006710570
          Length = 5195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3248 HVRGIKKL 3255
>XP_006710569
          Length = 5212

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3265 HVRGIKKL 3272
>XP_006710568
          Length = 5221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3274 HVRGIKKL 3281
>XP_006710567
          Length = 5222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3275 HVRGIKKL 3282
>XP_006710566
          Length = 5222

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3275 HVRGIKKL 3282
>XP_005245859
          Length = 5223

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    NVRGMRKL 9
            +VRG++KL
Sbjct: 3276 HVRGIKKL 3283
>NP_001005482
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QNVRGMRK 8
           ++VRG+RK
Sbjct: 234 KSVRGVRK 241
>NP_001004759
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   NVRGMR 7
           NVRG+R
Sbjct: 345 NVRGLR 350
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.138    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 428,685
Number of extensions: 616
Number of successful extensions: 54
Number of sequences better than 10000.0: 53
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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