Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005179                                                              19     869
NP_733762                                                              19     869
XP_006713860                                                           19     869
XP_006713859                                                           19     869
XP_005247910                                                           19     869
XP_005247909                                                           19     869
NP_037412                                                              18    1135
XP_005273918                                                           18    1135
NP_619542                                                              17    3301
XP_005274600                                                           17    3301
NP_001273560                                                           17    4312
NP_938205                                                              17    4312
NP_037413                                                              17    4312
XP_005260739                                                           17    4312
XP_006719923                                                           17    4312
XP_006719922                                                           17    4312
XP_006719920                                                           17    4312
XP_005266554                                                           17    4312
XP_005266553                                                           17    4312
XP_006719919                                                           17    4312
XP_005266551                                                           17    4312
XP_005266550                                                           17    4312
XP_005266549                                                           17    4312
XP_006719918                                                           17    4312
XP_005266548                                                           17    4312
NP_775752                                                              16    5631
XP_005259550                                                           16    5631
NP_031399                                                              16    7355
NP_001255968                                                           16    7355
NP_004066                                                              15    9605
NP_001155244                                                           15    9605
NP_000860                                                              15    9605
NP_998786                                                              15    9605
NP_065772                                                              15    9605
XP_006724837                                                           15    9605
>NP_005179
          Length = 906

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NLTKLSLI
Sbjct: 149 RNLTKLSLI 157
>NP_733762
          Length = 982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NLTKLSLI
Sbjct: 141 RNLTKLSLI 149
>XP_006713860
          Length = 914

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NLTKLSLI
Sbjct: 169 RNLTKLSLI 177
>XP_006713859
          Length = 966

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NLTKLSLI
Sbjct: 169 RNLTKLSLI 177
>XP_005247910
          Length = 982

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NLTKLSLI
Sbjct: 141 RNLTKLSLI 149
>XP_005247909
          Length = 1010

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NLTKLSLI
Sbjct: 169 RNLTKLSLI 177
>NP_037412
          Length = 674

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           QNLT+LSL+
Sbjct: 248 QNLTELSLV 256
>XP_005273918
          Length = 674

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           QNLT+LSL+
Sbjct: 248 QNLTELSLV 256
>NP_619542
          Length = 1041

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  QNLTKLSL 8
          QNLTK++L
Sbjct: 87 QNLTKINL 94
>XP_005274600
          Length = 1059

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QNLTK++L
Sbjct: 105 QNLTKINL 112
>NP_001273560
          Length = 1009

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 664 QNVTKLSV 671
>NP_938205
          Length = 649

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   NLTKLSLI 9
           NLT+LSL+
Sbjct: 226 NLTELSLV 233
>NP_037413
          Length = 649

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   NLTKLSLI 9
           NLT+LSL+
Sbjct: 226 NLTELSLV 233
>XP_005260739
          Length = 649

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   NLTKLSLI 9
           NLT+LSL+
Sbjct: 226 NLTELSLV 233
>XP_006719923
          Length = 734

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 389 QNVTKLSV 396
>XP_006719922
          Length = 942

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 677 QNVTKLSV 684
>XP_006719920
          Length = 999

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 677 QNVTKLSV 684
>XP_005266554
          Length = 1000

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 677 QNVTKLSV 684
>XP_005266553
          Length = 1007

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 662 QNVTKLSV 669
>XP_006719919
          Length = 1009

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 664 QNVTKLSV 671
>XP_005266551
          Length = 1012

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 667 QNVTKLSV 674
>XP_005266550
          Length = 1017

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 672 QNVTKLSV 679
>XP_005266549
          Length = 1019

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 674 QNVTKLSV 681
>XP_006719918
          Length = 1021

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 676 QNVTKLSV 683
>XP_005266548
          Length = 1022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QNLTKLSL 8
           QN+TKLS+
Sbjct: 677 QNVTKLSV 684
>NP_775752
          Length = 679

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LTKLSLI 9
           LTKLSLI
Sbjct: 331 LTKLSLI 337
>XP_005259550
          Length = 666

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   LTKLSLI 9
           LTKLSLI
Sbjct: 331 LTKLSLI 337
>NP_031399
          Length = 582

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NL+KLS++
Sbjct: 212 KNLSKLSML 220
>NP_001255968
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QNLTKLSLI 9
           +NL+KLS++
Sbjct: 212 KNLSKLSML 220
>NP_004066
          Length = 586

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NLTKLSL 8
          N+TKLS+
Sbjct: 92 NITKLSI 98
>NP_001155244
          Length = 463

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  NLTKLSL 8
          N+TKLS+
Sbjct: 89 NITKLSI 95
>NP_000860
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NLTKLSL 8
           N+TKLS+
Sbjct: 110 NITKLSI 116
>NP_998786
          Length = 516

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   NLTKLSL 8
           N+TKLS+
Sbjct: 110 NITKLSI 116
>NP_065772
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NLTKLSLI 9
            NLTK+S +
Sbjct: 1531 NLTKISYV 1538
>XP_006724837
          Length = 1533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NLTKLSLI 9
            NLTK+S +
Sbjct: 1355 NLTKISYV 1362
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.131    0.309 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 290,035
Number of extensions: 331
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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