Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              19     665
XP_005262424                                                           19     665
NP_001137499                                                           16    5631
NP_055249                                                              16    5631
NP_001137500                                                           16    5631
NP_001137498                                                           16    5631
XP_006717120                                                           16    5631
XP_006717119                                                           16    5631
XP_005266123                                                           16    5631
NP_789786                                                              16    7355
NP_001264282                                                           16    7355
XP_005276824                                                           16    7355
XP_005276823                                                           16    7355
XP_005276822                                                           16    7355
XP_006726278                                                           16    7355
XP_005276821                                                           16    7355
XP_006726277                                                           16    7355
XP_005276820                                                           16    7355
XP_005276819                                                           16    7355
XP_005276818                                                           16    7355
XP_005248520                                                           16    7355
XP_005248519                                                           16    7355
XP_005248518                                                           16    7355
XP_006714633                                                           16    7355
XP_005248517                                                           16    7355
XP_006714632                                                           16    7355
XP_005248516                                                           16    7355
XP_005248515                                                           16    7355
XP_005248514                                                           16    7355
XP_006714171                                                           16    7355
NP_001092874                                                           15    9605
NP_001092875                                                           15    9605
NP_001153632                                                           15    9605
NP_001153633                                                           15    9605
NP_932173                                                              15    9605
NP_000326                                                              15    9605
NP_008879                                                              15    9605
NP_001032                                                              15    9605
NP_004659                                                              15    9605
NP_001099001                                                           15    9605
NP_065870                                                              15    9605
NP_001156001                                                           15    9605
XP_006716232                                                           15    9605
XP_006716231                                                           15    9605
XP_005248808                                                           15    9605
XP_005248806                                                           15    9605
XP_005248805                                                           15    9605
XP_005248802                                                           15    9605
XP_005248801                                                           15    9605
XP_005248800                                                           15    9605
XP_006713346                                                           15    9605
XP_006713345                                                           15    9605
NP_001005185                                                           15    9605
NP_703146                                                              15    9605
>NP_722576
          Length = 3080

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QMVEFSVLG 9
            QMVEF VLG
Sbjct: 1859 QMVEFPVLG 1867
>XP_005262424
          Length = 2875

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QMVEFSVLG 9
            QMVEF VLG
Sbjct: 1654 QMVEFPVLG 1662
>NP_001137499
          Length = 521

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 195 RLIEFDILG 203
>NP_055249
          Length = 597

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 229 RLIEFDILG 237
>NP_001137500
          Length = 590

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 229 RLIEFDILG 237
>NP_001137498
          Length = 606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 229 RLIEFDILG 237
>XP_006717120
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 160 RLIEFDILG 168
>XP_006717119
          Length = 615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 229 RLIEFDILG 237
>XP_005266123
          Length = 624

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QMVEFSVLG 9
           +++EF +LG
Sbjct: 229 RLIEFDILG 237
>NP_789786
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 463 EFSVLG 468
>NP_001264282
          Length = 374

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VEFSVLG 9
           +EFS+LG
Sbjct: 284 IEFSMLG 290
>XP_005276824
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005276823
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005276822
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 373 EFSVLG 378
>XP_006726278
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 386 EFSVLG 391
>XP_005276821
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 390 EFSVLG 395
>XP_006726277
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 458 EFSVLG 463
>XP_005276820
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 462 EFSVLG 467
>XP_005276819
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_005276818
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_005248520
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005248519
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 338 EFSVLG 343
>XP_005248518
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 373 EFSVLG 378
>XP_006714633
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 386 EFSVLG 391
>XP_005248517
          Length = 438

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 390 EFSVLG 395
>XP_006714632
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 458 EFSVLG 463
>XP_005248516
          Length = 510

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 462 EFSVLG 467
>XP_005248515
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_005248514
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EFSVLG 9
           EFSVLG
Sbjct: 463 EFSVLG 468
>XP_006714171
          Length = 206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VEFSVLG 9
           +EFS+LG
Sbjct: 126 IEFSMLG 132
>NP_001092874
          Length = 2016

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1807 QFIEYSVL 1814
>NP_001092875
          Length = 1998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1789 QFIEYSVL 1796
>NP_001153632
          Length = 1983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1774 QFIEYSVL 1781
>NP_001153633
          Length = 1962

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1753 QFIEYSVL 1760
>NP_932173
          Length = 2016

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1807 QFIEYSVL 1814
>NP_000326
          Length = 2015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1806 QFIEYSVL 1813
>NP_008879
          Length = 471

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MVEFSV 7
           MVEFSV
Sbjct: 129 MVEFSV 134
>NP_001032
          Length = 1827

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   MVEFSVLG 9
           M+EFS+ G
Sbjct: 619 MLEFSLFG 626

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MVEFSVLG 9
            M+EFS+ G
Sbjct: 1515 MMEFSLFG 1522
>NP_004659
          Length = 1857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MVEFSVLG 9
            M+EFS+ G
Sbjct: 1541 MMEFSLFG 1548
>NP_001099001
          Length = 1319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>NP_065870
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>NP_001156001
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_006716232
          Length = 2753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MVEFSVLG 9
            M+EFS+ G
Sbjct: 1541 MMEFSLFG 1548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MVEFSVLG 9
            M+EFS+ G
Sbjct: 2437 MMEFSLFG 2444
>XP_006716231
          Length = 2753

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MVEFSVLG 9
            M+EFS+ G
Sbjct: 1541 MMEFSLFG 1548

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    MVEFSVLG 9
            M+EFS+ G
Sbjct: 2437 MMEFSLFG 2444
>XP_005248808
          Length = 1121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248806
          Length = 1317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248805
          Length = 1345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248802
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248801
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_005248800
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MVEFSV 7
          MVEFSV
Sbjct: 7  MVEFSV 12
>XP_006713346
          Length = 2016

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1807 QFIEYSVL 1814
>XP_006713345
          Length = 2016

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    QMVEFSVL 8
            Q +E+SVL
Sbjct: 1807 QFIEYSVL 1814
>NP_001005185
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QMVEFSVLG 9
          Q+ EF +LG
Sbjct: 8  QVAEFIILG 16
>NP_703146
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  MVEFSVLG 9
          +VEF++LG
Sbjct: 9  VVEFTLLG 16
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.140    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 379,092
Number of extensions: 546
Number of successful extensions: 57
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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