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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_001254652                                                           17    4312
XP_005259249                                                           17    4312
XP_005259248                                                           17    4312
NP_001185731                                                           16    5631
NP_001185730                                                           16    5631
NP_006036                                                              16    5631
NP_003751                                                              16    5631
NP_001012989                                                           16    7355
NP_001013859                                                           15    9605
NP_001013858                                                           15    9605
NP_003541                                                              15    9605
NP_004757                                                              15    9605
XP_005249935                                                           15    9605
XP_005249934                                                           15    9605
XP_006715851                                                           15    9605
XP_005249933                                                           15    9605

>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QMLEMEVEL 9
            QMLEM+VEL
Sbjct: 3132 QMLEMQVEL 3140



>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QMLEMEVEL 9
            QMLEM+VEL
Sbjct: 3124 QMLEMQVEL 3132



>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QMLEMEVEL 9
            QMLEM+VEL
Sbjct: 3085 QMLEMQVEL 3093



>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QMLEMEVEL 9
            QMLEM+VEL
Sbjct: 3164 QMLEMQVEL 3172



>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QMLEMEVEL 9
            QMLEM+VEL
Sbjct: 3177 QMLEMQVEL 3185



>NP_001254652
          Length = 512

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  QMLEMEVE 8
          Q+LE+EVE
Sbjct: 27 QVLELEVE 34



>XP_005259249
          Length = 512

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  QMLEMEVE 8
          Q+LE+EVE
Sbjct: 27 QVLELEVE 34



>XP_005259248
          Length = 520

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  QMLEMEVE 8
          Q+LE+EVE
Sbjct: 35 QVLELEVE 42



>NP_001185731
          Length = 1591

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QMLEMEVEL 9
           +MLE E+EL
Sbjct: 527 EMLEAELEL 535



>NP_001185730
          Length = 1621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QMLEMEVEL 9
           +MLE E+EL
Sbjct: 520 EMLEAELEL 528



>NP_006036
          Length = 1047

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QMLEMEVEL 9
           + LEME+EL
Sbjct: 637 ESLEMEMEL 645



>NP_003751
          Length = 1585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QMLEMEVEL 9
           +MLE E+EL
Sbjct: 521 EMLEAELEL 529



>NP_001012989
          Length = 171

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           ++E+EVEL
Sbjct: 116 LMELEVEL 123



>NP_001013859
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 472 MLEMELKM 479



>NP_001013858
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 472 MLEMELKM 479



>NP_003541
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 472 MLEMELKM 479



>NP_004757
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           +LE+EV+L
Sbjct: 877 LLELEVDL 884



>XP_005249935
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 425 MLEMELKM 432



>XP_005249934
          Length = 671

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 425 MLEMELKM 432



>XP_006715851
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 472 MLEMELKM 479



>XP_005249933
          Length = 718

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MLEMEVEL 9
           MLEME+++
Sbjct: 472 MLEMELKM 479



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.130    0.294 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 449,392
Number of extensions: 620
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)