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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1135
XP_005267290                                                           18    1135
NP_001058                                                              16    7355
NP_001059                                                              16    7355
XP_005257689                                                           16    7355
XP_005265484                                                           16    7355
NP_001275929                                                           15    9605
NP_001275930                                                           15    9605
NP_705837                                                              15    9605
XP_006724226                                                           15    9605
XP_006724225                                                           15    9605

>NP_005091
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QLSTINSVA 9
          QLSTIN VA
Sbjct: 61 QLSTINGVA 69



>XP_005267290
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  QLSTINSVA 9
          QLSTIN VA
Sbjct: 61 QLSTINGVA 69



>NP_001058
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLSTINSVA 9
           Q+S +NS+A
Sbjct: 310 QISFVNSIA 318



>NP_001059
          Length = 1621

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLSTINSVA 9
           Q+S +NS+A
Sbjct: 326 QISFVNSIA 334



>XP_005257689
          Length = 1519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLSTINSVA 9
           Q+S +NS+A
Sbjct: 298 QISFVNSIA 306



>XP_005265484
          Length = 1626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLSTINSVA 9
           Q+S +NS+A
Sbjct: 331 QISFVNSIA 339



>NP_001275929
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSTINSVA 9
           LST+NS A
Sbjct: 171 LSTVNSSA 178



>NP_001275930
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSTINSVA 9
           LST+NS A
Sbjct: 171 LSTVNSSA 178



>NP_705837
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSTINSVA 9
           LST+NS A
Sbjct: 180 LSTVNSSA 187



>XP_006724226
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSTINSVA 9
           LST+NS A
Sbjct: 171 LSTVNSSA 178



>XP_006724225
          Length = 802

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LSTINSVA 9
           LST+NS A
Sbjct: 171 LSTVNSSA 178



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.111    0.246 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 256,613
Number of extensions: 221
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)