Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1935
XP_005266703                                                           18    1935
NP_001128669                                                           17    4312
XP_005265157                                                           17    4312
XP_005265156                                                           17    4312
XP_006713187                                                           17    4312
NP_835467                                                              17    4312
NP_067681                                                              16    7355
NP_004531                                                              16    7355
NP_009185                                                              16    7355
XP_006724071                                                           16    7355
XP_006724070                                                           16    7355
NP_004360                                                              15    9605
NP_476508                                                              15    9605
NP_476507                                                              15    9605
NP_001155000                                                           15    9605
NP_892030                                                              15    9605
XP_006725244                                                           15    9605
XP_006725241                                                           15    9605
XP_006725240                                                           15    9605
XP_006720456                                                           15    9605
XP_005254219                                                           15    9605
XP_005246123                                                           15    9605
XP_005246122                                                           15    9605
XP_003846701                                                           15    9605
>NP_443179
          Length = 2170

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLSIGKSKV 9
            Q SIGKSKV
Sbjct: 2142 QPSIGKSKV 2150
>XP_005266703
          Length = 1501

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLSIGKSKV 9
            Q SIGKSKV
Sbjct: 1473 QPSIGKSKV 1481
>NP_001128669
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSIGKSK 8
           LS+GKSK
Sbjct: 107 LSVGKSK 113
>XP_005265157
          Length = 577

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LSIGKSK 8
          LS+GKSK
Sbjct: 90 LSVGKSK 96
>XP_005265156
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSIGKSK 8
           LS+GKSK
Sbjct: 107 LSVGKSK 113
>XP_006713187
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSIGKSK 8
           LS+GKSK
Sbjct: 107 LSVGKSK 113
>NP_835467
          Length = 594

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LSIGKSK 8
           LS+GKSK
Sbjct: 107 LSVGKSK 113
>NP_067681
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2 LSIGKSKV 9
         + IGKSK+
Sbjct: 1 MGIGKSKI 8
>NP_004531
          Length = 837

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QLSIGKSK 8
          +LS+G+SK
Sbjct: 31 ELSVGESK 38
>NP_009185
          Length = 521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLSIGKSKV 9
           Q+SIG+ K+
Sbjct: 219 QMSIGRGKL 227
>XP_006724071
          Length = 699

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QLSIGKSK 8
          +LS+G+SK
Sbjct: 31 ELSVGESK 38
>XP_006724070
          Length = 727

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QLSIGKSK 8
          +LS+G+SK
Sbjct: 31 ELSVGESK 38
>NP_004360
          Length = 3177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QLSIGKSKV 9
            +L+IG SKV
Sbjct: 1465 RLNIGPSKV 1473
>NP_476508
          Length = 2971

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QLSIGKSKV 9
            +L+IG SKV
Sbjct: 1259 RLNIGPSKV 1267
>NP_476507
          Length = 2570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QLSIGKSKV 9
           +L+IG SKV
Sbjct: 858 RLNIGPSKV 866
>NP_001155000
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LSIGKSKV 9
           LS+GK+K+
Sbjct: 262 LSLGKNKL 269
>NP_892030
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QLSIGKSK 8
           +LS+G+SK
Sbjct: 141 RLSVGQSK 148
>XP_006725244
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QLSIGKSK 8
           +LS+G+SK
Sbjct: 123 RLSVGQSK 130
>XP_006725241
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QLSIGKSK 8
           +LS+G+SK
Sbjct: 123 RLSVGQSK 130
>XP_006725240
          Length = 500

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QLSIGKSK 8
           +LS+G+SK
Sbjct: 141 RLSVGQSK 148
>XP_006720456
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QLSIGKSK 8
           +LS+G+SK
Sbjct: 141 RLSVGQSK 148
>XP_005254219
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QLSIGKSK 8
           +LS+G+SK
Sbjct: 141 RLSVGQSK 148
>XP_005246123
          Length = 2770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QLSIGKSKV 9
            +L+IG SKV
Sbjct: 1058 RLNIGPSKV 1066
>XP_005246122
          Length = 2977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QLSIGKSKV 9
            +L+IG SKV
Sbjct: 1265 RLNIGPSKV 1273
>XP_003846701
          Length = 143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QLSIGKSK 8
           QLS+GK +
Sbjct: 131 QLSVGKKQ 138
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.305    0.124    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 410,212
Number of extensions: 397
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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