Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_073153                                                              17    4312
XP_006726447                                                           17    4312
XP_005276263                                                           17    4312
XP_006712754                                                           17    4312
XP_006712753                                                           17    4312
XP_005264736                                                           17    4312
NP_055692                                                              16    5631
NP_208384                                                              16    5631
NP_001032412                                                           16    5631
NP_001008392                                                           16    5631
XP_005257947                                                           16    5631
XP_005257946                                                           16    5631
XP_005257945                                                           16    5631
XP_006722277                                                           16    5631
NP_005795                                                              16    7355
NP_775753                                                              15    9605
NP_055515                                                              15    9605
NP_006442                                                              15    9605
NP_899152                                                              15    9605
NP_877590                                                              15    9605
XP_006725309                                                           15    9605
XP_006725308                                                           15    9605
XP_006725305                                                           15    9605
XP_005255772                                                           15    9605
XP_005255771                                                           15    9605
XP_005255770                                                           15    9605
XP_005248287                                                           15    9605
NP_001106212                                                           15    9605
NP_001135744                                                           15    9605
NP_612144                                                              15    9605
>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLNGQVTKL 9
            QLN QVTKL
Sbjct: 2296 QLNEQVTKL 2304
>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLNGQVTKL 9
            QLN QVTKL
Sbjct: 2288 QLNEQVTKL 2296
>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLNGQVTKL 9
            QLN QVTKL
Sbjct: 2249 QLNEQVTKL 2257
>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLNGQVTKL 9
            QLN QVTKL
Sbjct: 2328 QLNEQVTKL 2336
>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLNGQVTKL 9
            QLN QVTKL
Sbjct: 2341 QLNEQVTKL 2349
>NP_073153
          Length = 1944

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LNGQVTKL 9
            LNG +TKL
Sbjct: 1181 LNGHLTKL 1188
>XP_006726447
          Length = 368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LNGQVTKL 9
           LNG +TKL
Sbjct: 166 LNGHLTKL 173
>XP_005276263
          Length = 368

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LNGQVTKL 9
           LNG +TKL
Sbjct: 166 LNGHLTKL 173
>XP_006712754
          Length = 1784

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LNGQVTKL 9
            LNG +TKL
Sbjct: 1021 LNGHLTKL 1028
>XP_006712753
          Length = 1846

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LNGQVTKL 9
            LNG +TKL
Sbjct: 1083 LNGHLTKL 1090
>XP_005264736
          Length = 671

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LNGQVTKL 9
           LNG +TKL
Sbjct: 469 LNGHLTKL 476
>NP_055692
          Length = 1942

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNGQVTK 8
           LNG VTK
Sbjct: 124 LNGMVTK 130
>NP_208384
          Length = 2080

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LNGQVTKL 9
           +NG VTK+
Sbjct: 650 INGSVTKI 657
>NP_001032412
          Length = 2649

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    LNGQVTKL 9
            +NG VTK+
Sbjct: 1219 INGSVTKI 1226
>NP_001008392
          Length = 1079

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   QLNGQVTKL 9
           +LNG++ K+
Sbjct: 353 ELNGEINKI 361
>XP_005257947
          Length = 1935

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNGQVTK 8
           LNG VTK
Sbjct: 124 LNGMVTK 130
>XP_005257946
          Length = 1943

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNGQVTK 8
           LNG VTK
Sbjct: 124 LNGMVTK 130
>XP_005257945
          Length = 1969

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNGQVTK 8
           LNG VTK
Sbjct: 124 LNGMVTK 130
>XP_006722277
          Length = 1970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LNGQVTK 8
           LNG VTK
Sbjct: 124 LNGMVTK 130
>NP_005795
          Length = 427

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LNGQVTKL 9
           +NGQVT L
Sbjct: 109 VNGQVTVL 116
>NP_775753
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLNGQVTKL 9
           Q+NG+V +L
Sbjct: 146 QINGRVRQL 154
>NP_055515
          Length = 756

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 683 ELNGQIITL 691
>NP_006442
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NGQVTK 8
           NGQVT+
Sbjct: 287 NGQVTR 292
>NP_899152
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NGQVTK 8
           NGQVT+
Sbjct: 175 NGQVTR 180
>NP_877590
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NGQVTK 8
           NGQVT+
Sbjct: 208 NGQVTR 213
>XP_006725309
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 418 ELNGQIITL 426
>XP_006725308
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 432 ELNGQIITL 440
>XP_006725305
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 728 ELNGQIITL 736
>XP_005255772
          Length = 491

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 418 ELNGQIITL 426
>XP_005255771
          Length = 505

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 432 ELNGQIITL 440
>XP_005255770
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 728 ELNGQIITL 736
>XP_005248287
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NGQVTK 8
           NGQVT+
Sbjct: 175 NGQVTR 180
>NP_001106212
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QLNGQVTK 8
           +LNG+V K
Sbjct: 504 KLNGEVAK 511
>NP_001135744
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LNGQ+  L
Sbjct: 387 ELNGQIITL 395
>NP_612144
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QLNGQVTKL 9
           +LN Q TKL
Sbjct: 324 ELNHQFTKL 332
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.129    0.330 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 495,110
Number of extensions: 584
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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