Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              20     509
NP_001035957                                                           20     509
XP_006721990                                                           20     509
XP_006721989                                                           20     509
XP_005258041                                                           20     509
XP_006721988                                                           20     509
XP_006721987                                                           20     509
XP_006721986                                                           20     509
XP_005258040                                                           20     509
XP_006721985                                                           20     509
NP_001269607                                                           16    5631
NP_001269606                                                           16    5631
NP_000457                                                              16    5631
NP_006761                                                              16    5631
NP_057314                                                              16    5631
NP_002220                                                              16    5631
XP_005250490                                                           16    5631
XP_005250486                                                           16    5631
NP_001849                                                              16    7355
NP_780775                                                              16    7355
NP_001230199                                                           16    7355
NP_055176                                                              16    7355
NP_001231627                                                           16    7355
NP_004917                                                              16    7355
NP_060718                                                              16    7355
NP_001231630                                                           16    7355
NP_066933                                                              15    9605
NP_000570                                                              15    9605
NP_001093638                                                           15    9605
NP_631918                                                              15    9605
NP_005984                                                              15    9605
NP_005651                                                              15    9605
XP_006726460                                                           15    9605
XP_006722353                                                           15    9605
XP_005256453                                                           15    9605
XP_005251116                                                           15    9605
XP_006716714                                                           15    9605
XP_005275982                                                           15    9605
>NP_000258
          Length = 2818

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1674 RLLTGLKGS 1682
>NP_001035957
          Length = 2839

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1695 RLLTGLKGS 1703
>XP_006721990
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1705 RLLTGLKGS 1713
>XP_006721989
          Length = 2823

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1705 RLLTGLKGS 1713
>XP_005258041
          Length = 2836

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1674 RLLTGLKGS 1682
>XP_006721988
          Length = 2846

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1684 RLLTGLKGS 1692
>XP_006721987
          Length = 2849

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1705 RLLTGLKGS 1713
>XP_006721986
          Length = 2854

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1692 RLLTGLKGS 1700
>XP_005258040
          Length = 2857

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1695 RLLTGLKGS 1703
>XP_006721985
          Length = 2867

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QLLTGLKGS 9
            +LLTGLKGS
Sbjct: 1705 RLLTGLKGS 1713
>NP_001269607
          Length = 1075

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LLTGLKGS 9
           LLTG KGS
Sbjct: 388 LLTGGKGS 395
>NP_001269606
          Length = 1226

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LLTGLKGS 9
           LLTG KGS
Sbjct: 596 LLTGGKGS 603
>NP_000457
          Length = 1283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LLTGLKGS 9
           LLTG KGS
Sbjct: 596 LLTGGKGS 603
>NP_006761
          Length = 520

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TGLKGS 9
           TGLKGS
Sbjct: 345 TGLKGS 350
>NP_057314
          Length = 521

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  LTGLKG 8
          LTGLKG
Sbjct: 47 LTGLKG 52
>NP_002220
          Length = 347

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QLLTGLKG 8
           QLL G+KG
Sbjct: 337 QLLLGVKG 344
>XP_005250490
          Length = 700

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  LLTGLKGS 9
          LLTG KGS
Sbjct: 13 LLTGGKGS 20
>XP_005250486
          Length = 1243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LLTGLKGS 9
           LLTG KGS
Sbjct: 556 LLTGGKGS 563
>NP_001849
          Length = 1142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LTGLKGS 9
           LTG+KG+
Sbjct: 972 LTGMKGA 978
>NP_780775
          Length = 1026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LLTGLKGS 9
           +LTGL+G+
Sbjct: 862 ILTGLEGN 869
>NP_001230199
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LLTGLKGS 9
           +LTGL+G+
Sbjct: 936 ILTGLEGN 943
>NP_055176
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   LLTGLKGS 9
           +LTGL+G+
Sbjct: 936 ILTGLEGN 943
>NP_001231627
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QLLTGLKG 8
          QLL+ LKG
Sbjct: 66 QLLSSLKG 73
>NP_004917
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QLLTGLKG 8
          QLL+ LKG
Sbjct: 66 QLLSSLKG 73
>NP_060718
          Length = 605

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QLLTGLKGS 9
          Q +TG++GS
Sbjct: 22 QAVTGVRGS 30
>NP_001231630
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QLLTGLKG 8
           QLL+ LKG
Sbjct: 135 QLLSSLKG 142
>NP_066933
          Length = 1796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LTGLKG 8
            LTG+KG
Sbjct: 1694 LTGIKG 1699
>NP_000570
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QLLTGL 6
           QLLTGL
Sbjct: 102 QLLTGL 107
>NP_001093638
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QLLTGL 6
           QLLTGL
Sbjct: 102 QLLTGL 107
>NP_631918
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLLTGLK 7
           QLL GLK
Sbjct: 180 QLLNGLK 186
>NP_005984
          Length = 1192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLLTGLK 7
           QLL GLK
Sbjct: 364 QLLVGLK 370
>NP_005651
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QLLTGLKGS 9
           +LLT +KGS
Sbjct: 388 KLLTKVKGS 396
>XP_006726460
          Length = 247

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QLLTGL 6
           QLLTGL
Sbjct: 215 QLLTGL 220
>XP_006722353
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLLTGLK 7
           QLL GLK
Sbjct: 364 QLLVGLK 370
>XP_005256453
          Length = 1175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLLTGLK 7
           QLL GLK
Sbjct: 347 QLLVGLK 353
>XP_005251116
          Length = 1780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LTGLKG 8
            LTG+KG
Sbjct: 1694 LTGIKG 1699
>XP_006716714
          Length = 1796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    LTGLKG 8
            LTG+KG
Sbjct: 1694 LTGIKG 1699
>XP_005275982
          Length = 187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   QLLTGL 6
           QLLTGL
Sbjct: 155 QLLTGL 160
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.310    0.136    0.355 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 528,712
Number of extensions: 1186
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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