Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000236                                                              21     175
NP_001120972                                                           21     175
XP_006716052                                                           21     175
XP_006716051                                                           21     175
NP_001229679                                                           17    4312
NP_001244066                                                           16    5631
NP_777576                                                              16    7355
NP_997235                                                              15    9605
NP_115820                                                              15    9605
NP_001145                                                              15    9605
NP_001119595                                                           15    9605
NP_001167560                                                           15    9605
NP_114423                                                              15    9605
NP_001167561                                                           15    9605
NP_061950                                                              15    9605
NP_061949                                                              15    9605
NP_066307                                                              15    9605
NP_059982                                                              15    9605
NP_974487                                                              15    9605
XP_005260914                                                           15    9605
XP_005260913                                                           15    9605
XP_006722754                                                           15    9605
XP_005259877                                                           15    9605
XP_005258175                                                           15    9605
XP_006714247                                                           15    9605
XP_005262992                                                           15    9605
NP_055790                                                              15    9605
>NP_000236
          Length = 1390

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QLGASLNDD 9
           Q+GASLNDD
Sbjct: 332 QIGASLNDD 340
>NP_001120972
          Length = 1408

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QLGASLNDD 9
           Q+GASLNDD
Sbjct: 332 QIGASLNDD 340
>XP_006716052
          Length = 1409

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QLGASLNDD 9
           Q+GASLNDD
Sbjct: 351 QIGASLNDD 359
>XP_006716051
          Length = 1427

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QLGASLNDD 9
           Q+GASLNDD
Sbjct: 351 QIGASLNDD 359
>NP_001229679
          Length = 245

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QLGASLND 8
          +LGA LND
Sbjct: 87 KLGAELND 94
>NP_001244066
          Length = 903

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   GASLNDD 9
           GAS NDD
Sbjct: 200 GASQNDD 206
>NP_777576
          Length = 1749

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QLGASLND 8
           Q+GASL D
Sbjct: 696 QIGASLMD 703
>NP_997235
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LGASLNDD 9
           +GA  NDD
Sbjct: 209 IGAQSNDD 216
>NP_115820
          Length = 1834

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLGASLNDD 9
           ++GAS +DD
Sbjct: 278 RVGASAHDD 286
>NP_001145
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLGASLNDD 9
           + G+SL DD
Sbjct: 132 EYGSSLEDD 140
>NP_001119595
          Length = 361

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLGASLNDD 9
           ++GA L DD
Sbjct: 140 KVGADLQDD 148
>NP_001167560
          Length = 875

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LGASLN 7
           LGASLN
Sbjct: 524 LGASLN 529
>NP_114423
          Length = 891

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LGASLN 7
           LGASLN
Sbjct: 540 LGASLN 545
>NP_001167561
          Length = 918

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LGASLN 7
           LGASLN
Sbjct: 567 LGASLN 572
>NP_061950
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  QLGASLN 7
          QLG SLN
Sbjct: 65 QLGRSLN 71
>NP_061949
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  QLGASLN 7
          QLG SLN
Sbjct: 65 QLGKSLN 71
>NP_066307
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  QLGASLN 7
          QLG SLN
Sbjct: 65 QLGRSLN 71
>NP_059982
          Length = 443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLGASLNDD 9
           ++GA L DD
Sbjct: 222 KVGADLQDD 230
>NP_974487
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QLGASLNDD 9
           ++GA L DD
Sbjct: 243 KVGADLQDD 251
>XP_005260914
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LGASLN 7
           LGASLN
Sbjct: 505 LGASLN 510
>XP_005260913
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LGASLN 7
           LGASLN
Sbjct: 647 LGASLN 652
>XP_006722754
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QLGASLNDD 9
           +LGASL+ D
Sbjct: 868 KLGASLSAD 876
>XP_005259877
          Length = 1394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QLGASLNDD 9
            +LGASL+ D
Sbjct: 1093 KLGASLSAD 1101
>XP_005258175
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  QLGASLNDD 9
          ++GA L DD
Sbjct: 41 KVGADLQDD 49
>XP_006714247
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  LGASLNDD 9
          +GA  NDD
Sbjct: 77 IGAQSNDD 84
>XP_005262992
          Length = 524

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LGASLNDD 9
           +GA  NDD
Sbjct: 209 IGAQSNDD 216
>NP_055790
          Length = 1570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    QLGASLNDD 9
            +LGASL+ D
Sbjct: 1269 KLGASLSAD 1277
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.128    0.339 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 524,886
Number of extensions: 889
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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