Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     869
NP_001075109                                                           19     869
NP_000121                                                              16    5631
NP_001123492                                                           16    5631
NP_001123493                                                           16    5631
NP_116202                                                              16    5631
NP_001007472                                                           16    5631
NP_005168                                                              16    5631
NP_001073137                                                           16    5631
XP_006724407                                                           16    5631
XP_005261847                                                           16    5631
XP_005257520                                                           16    5631
XP_005257518                                                           16    5631
XP_005257516                                                           16    5631
XP_005254137                                                           16    5631
XP_006720017                                                           16    5631
NP_001092764                                                           16    5631
NP_001229679                                                           16    5631
NP_115823                                                              16    7355
XP_006722988                                                           16    7355
NP_742107                                                              15    9605
NP_004870                                                              15    9605
NP_001277064                                                           15    9605
NP_689899                                                              15    9605
NP_054772                                                              15    9605
NP_660204                                                              15    9605
NP_001020276                                                           15    9605
NP_997724                                                              15    9605
NP_115598                                                              15    9605
NP_001258671                                                           15    9605
NP_001258670                                                           15    9605
NP_071737                                                              15    9605
NP_001883                                                              15    9605
NP_878905                                                              15    9605
XP_006714631                                                           15    9605
XP_006714630                                                           15    9605
XP_005271975                                                           15    9605
XP_005271974                                                           15    9605
XP_005265446                                                           15    9605
XP_005265445                                                           15    9605
NP_005469                                                              15    9605
NP_001128350                                                           15    9605
>NP_008835
          Length = 4128

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLFSILFSG 9
            QLFS LFSG
Sbjct: 2807 QLFSSLFSG 2815
>NP_001075109
          Length = 4097

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLFSILFSG 9
            QLFS LFSG
Sbjct: 2807 QLFSSLFSG 2815
>NP_000121
          Length = 2224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           +LFSI F+G
Sbjct: 265 ELFSIHFNG 273
>NP_001123492
          Length = 838

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           ++FS +FSG
Sbjct: 445 EMFSTVFSG 453
>NP_001123493
          Length = 837

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           ++FS +FSG
Sbjct: 438 EMFSTVFSG 446
>NP_116202
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LFSILFSG 9
           +FS+LF G
Sbjct: 144 VFSVLFGG 151
>NP_001007472
          Length = 1707

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QLFSILFS 8
          Q+FS LFS
Sbjct: 15 QVFSFLFS 22
>NP_005168
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           ++FS +FSG
Sbjct: 438 EMFSTVFSG 446
>NP_001073137
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LFSILFSG 9
           +FS+LF G
Sbjct: 125 VFSVLFGG 132
>XP_006724407
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LFSILFS 8
          LFS+LFS
Sbjct: 15 LFSLLFS 21
>XP_005261847
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LFSILFS 8
          LFS+LFS
Sbjct: 15 LFSLLFS 21
>XP_005257520
          Length = 784

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           ++FS +FSG
Sbjct: 445 EMFSTVFSG 453
>XP_005257518
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           ++FS +FSG
Sbjct: 445 EMFSTVFSG 453
>XP_005257516
          Length = 844

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLFSILFSG 9
           ++FS +FSG
Sbjct: 445 EMFSTVFSG 453
>XP_005254137
          Length = 632

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LFSILFSG 9
           +FS+LF G
Sbjct: 144 VFSVLFGG 151
>XP_006720017
          Length = 741

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LFSILFSG 9
           +FS+LF G
Sbjct: 125 VFSVLFGG 132
>NP_001092764
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LFSILFS 8
          LFS+LFS
Sbjct: 15 LFSLLFS 21
>NP_001229679
          Length = 245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  LFSILFS 8
          LFSI+FS
Sbjct: 47 LFSIIFS 53
>NP_115823
          Length = 2809

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 296 FSVLFGG 302
>XP_006722988
          Length = 2818

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 305 FSVLFGG 311
>NP_742107
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   QLFSILFS 8
           QLF  LFS
Sbjct: 383 QLFHFLFS 390
>NP_004870
          Length = 340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LFSILF 7
           LFSILF
Sbjct: 265 LFSILF 270
>NP_001277064
          Length = 326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LFSILF 7
           LFSILF
Sbjct: 251 LFSILF 256
>NP_689899
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   QLFSILFSG 9
           +LF++ F G
Sbjct: 174 ELFNVFFGG 182
>NP_054772
          Length = 555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QLFSILFS 8
           Q+F ILF+
Sbjct: 471 QIFGILFT 478
>NP_660204
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLFSILF 7
           QLF ILF
Sbjct: 279 QLFRILF 285
>NP_001020276
          Length = 365

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLFSILF 7
           QLF ILF
Sbjct: 307 QLFRILF 313
>NP_997724
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3  FSILFS 8
          FSILFS
Sbjct: 35 FSILFS 40
>NP_115598
          Length = 757

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   FSILFS 8
           FSILFS
Sbjct: 678 FSILFS 683
>NP_001258671
          Length = 276

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLFSILF 7
           QLF ILF
Sbjct: 218 QLFRILF 224
>NP_001258670
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLFSILF 7
           QLF ILF
Sbjct: 279 QLFRILF 285
>NP_071737
          Length = 725

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 192 FSLLFEG 198
>NP_001883
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   QLFSILF 7
           QLF ILF
Sbjct: 279 QLFRILF 285
>NP_878905
          Length = 790

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 192 FSLLFEG 198
>XP_006714631
          Length = 329

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   QLFSILFSG 9
           +LF++ F G
Sbjct: 174 ELFNVFFGG 182
>XP_006714630
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   QLFSILFSG 9
           +LF++ F G
Sbjct: 174 ELFNVFFGG 182
>XP_005271975
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   QLFSILFSG 9
           +LF++ F G
Sbjct: 174 ELFNVFFGG 182
>XP_005271974
          Length = 358

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   QLFSILFSG 9
           +LF++ F G
Sbjct: 174 ELFNVFFGG 182
>XP_005265446
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 225 FSLLFEG 231
>XP_005265445
          Length = 823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 225 FSLLFEG 231
>NP_005469
          Length = 135

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  LFSILFS 8
          LFS+LF+
Sbjct: 10 LFSVLFA 16
>NP_001128350
          Length = 790

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   FSILFSG 9
           FS+LF G
Sbjct: 192 FSLLFEG 198
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.153    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 395,288
Number of extensions: 883
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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