Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_005272158                                                           18    1935
NP_114158                                                              18    1935
NP_008835                                                              17    3301
NP_001075109                                                           17    3301
NP_060141                                                              16    5631
NP_001034450                                                           16    7355
NP_001278310                                                           16    7355
NP_001120965                                                           16    7355
NP_066187                                                              16    7355
NP_001139                                                              16    7355
XP_006714257                                                           16    7355
XP_006714256                                                           16    7355
XP_006714255                                                           16    7355
XP_005263005                                                           16    7355
XP_006714254                                                           16    7355
XP_006714253                                                           16    7355
XP_005263002                                                           16    7355
XP_005262999                                                           16    7355
XP_006714252                                                           16    7355
XP_006714251                                                           16    7355
XP_006714250                                                           16    7355
NP_001093321                                                           15    9605
NP_001038945                                                           15    9605
NP_001193928                                                           15    9605
XP_006719269                                                           15    9605
>XP_005272158
          Length = 135

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLASRLLEL 9
           QLA RLLEL
Sbjct: 96  QLACRLLEL 104
>NP_114158
          Length = 254

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLASRLLEL 9
           QLA RLLEL
Sbjct: 96  QLACRLLEL 104
>NP_008835
          Length = 4128

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLASRLLEL 9
            QLAS LLEL
Sbjct: 1509 QLASGLLEL 1517
>NP_001075109
          Length = 4097

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QLASRLLEL 9
            QLAS LLEL
Sbjct: 1509 QLASGLLEL 1517
>NP_060141
          Length = 677

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QLASRLLE 8
           QLA+R+LE
Sbjct: 531 QLAARILE 538
>NP_001034450
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  QLASRLLEL 9
          Q  SRLLEL
Sbjct: 4  QAPSRLLEL 12
>NP_001278310
          Length = 474

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  QLASRLLEL 9
          Q  SRLLEL
Sbjct: 4  QAPSRLLEL 12
>NP_001120965
          Length = 1863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1017 LASRLIEV 1024
>NP_066187
          Length = 1872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1026 LASRLIEV 1033
>NP_001139
          Length = 3957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1026 LASRLIEV 1033
>XP_006714257
          Length = 4145

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1068 LASRLIEV 1075
>XP_006714256
          Length = 1901

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1088 LASRLIEV 1095
>XP_006714255
          Length = 1970

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1076 LASRLIEV 1083
>XP_005263005
          Length = 2019

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1058 LASRLIEV 1065
>XP_006714254
          Length = 2049

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1088 LASRLIEV 1095
>XP_006714253
          Length = 2069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1088 LASRLIEV 1095
>XP_005263002
          Length = 4072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1026 LASRLIEV 1033
>XP_005262999
          Length = 4147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1070 LASRLIEV 1077
>XP_006714252
          Length = 4153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1076 LASRLIEV 1083
>XP_006714251
          Length = 4165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1088 LASRLIEV 1095
>XP_006714250
          Length = 4190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    LASRLLEL 9
            LASRL+E+
Sbjct: 1080 LASRLIEV 1087
>NP_001093321
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  QLASRLLEL 9
          Q  SRLLEL
Sbjct: 4  QSPSRLLEL 12
>NP_001038945
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1  QLASRLLEL 9
          Q  SRLLEL
Sbjct: 4  QSPSRLLEL 12
>NP_001193928
          Length = 2069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLASRLLEL 9
           Q+A ++LEL
Sbjct: 730 QMADKILEL 738
>XP_006719269
          Length = 2054

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QLASRLLEL 9
           Q+A ++LEL
Sbjct: 730 QMADKILEL 738
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.137    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 295,963
Number of extensions: 235
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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