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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1482
NP_653080                                                              18    1482
XP_005267292                                                           18    1482
XP_005267291                                                           18    1482
XP_005267290                                                           18    1482
NP_005700                                                              16    5631
NP_710142                                                              16    5631
XP_005252782                                                           16    5631
XP_006718182                                                           16    5631

>NP_005091
          Length = 1782

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLAQKSAEQ 9
           Q AQKSAEQ
Sbjct: 863 QQAQKSAEQ 871



>NP_653080
          Length = 1684

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLAQKSAEQ 9
           Q AQKSAEQ
Sbjct: 765 QQAQKSAEQ 773



>XP_005267292
          Length = 1677

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLAQKSAEQ 9
           Q AQKSAEQ
Sbjct: 758 QQAQKSAEQ 766



>XP_005267291
          Length = 1684

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLAQKSAEQ 9
           Q AQKSAEQ
Sbjct: 765 QQAQKSAEQ 773



>XP_005267290
          Length = 1782

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QLAQKSAEQ 9
           Q AQKSAEQ
Sbjct: 863 QQAQKSAEQ 871



>NP_005700
          Length = 552

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QLAQKSAEQ 9
           ++AQK+AE+
Sbjct: 344 EIAQKAAEE 352



>NP_710142
          Length = 899

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QLAQKSAEQ 9
           ++AQK+AE+
Sbjct: 344 EIAQKAAEE 352



>XP_005252782
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QLAQKSAEQ 9
           ++AQK+AE+
Sbjct: 325 EIAQKAAEE 333



>XP_006718182
          Length = 541

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   QLAQKSAEQ 9
           ++AQK+AE+
Sbjct: 325 EIAQKAAEE 333



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.288   0.0979    0.208 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 235,778
Number of extensions: 377
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.0 bits)
S2: 28 (15.4 bits)