Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1482
XP_005266703                                                           18    1482
NP_056280                                                              17    3301
NP_001239085                                                           17    3301
XP_005248127                                                           16    5631
XP_005248126                                                           16    5631
XP_005248124                                                           16    5631
XP_005248123                                                           16    5631
XP_005248122                                                           16    5631
XP_005248121                                                           16    5631
XP_005248120                                                           16    5631
XP_006714059                                                           16    5631
XP_005248119                                                           16    5631
XP_005248118                                                           16    5631
XP_005248117                                                           16    5631
XP_005248116                                                           16    5631
XP_005248115                                                           16    5631
XP_005248114                                                           16    5631
NP_005076                                                              16    7355
NP_001230280                                                           16    7355
NP_057202                                                              16    7355
NP_004781                                                              16    7355
NP_695008                                                              16    7355
NP_695010                                                              16    7355
NP_695009                                                              16    7355
NP_006518                                                              16    7355
NP_055796                                                              16    7355
XP_006718456                                                           16    7355
XP_005272276                                                           16    7355
XP_005272275                                                           16    7355
XP_006717355                                                           16    7355
XP_005272274                                                           16    7355
XP_005272273                                                           16    7355
XP_005250069                                                           16    7355
XP_005250068                                                           16    7355
XP_005250067                                                           16    7355
NP_694948                                                              16    7355
NP_001182583                                                           15    9605
NP_115916                                                              15    9605
NP_001182582                                                           15    9605
NP_001076582                                                           15    9605
NP_852150                                                              15    9605
NP_005465                                                              15    9605
NP_001015053                                                           15    9605
NP_005885                                                              15    9605
XP_006721692                                                           15    9605
XP_005256964                                                           15    9605
XP_005256963                                                           15    9605
XP_005256962                                                           15    9605
XP_005256961                                                           15    9605
XP_006720705                                                           15    9605
XP_005245659                                                           15    9605
NP_001123920                                                           15    9605
NP_001094874                                                           15    9605
>NP_443179
          Length = 2170

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKQLSIGKS 9
            QKQ SIGKS
Sbjct: 2140 QKQPSIGKS 2148
>XP_005266703
          Length = 1501

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKQLSIGKS 9
            QKQ SIGKS
Sbjct: 1471 QKQPSIGKS 1479
>NP_056280
          Length = 1508

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    QKQLSIGKS 9
            +KQLS GKS
Sbjct: 1133 EKQLSEGKS 1141
>NP_001239085
          Length = 1507

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    QKQLSIGKS 9
            +KQLS GKS
Sbjct: 1132 EKQLSEGKS 1140
>XP_005248127
          Length = 1322

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248126
          Length = 1359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 371 KRLSIGKA 378
>XP_005248124
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 483 KRLSIGKA 490
>XP_005248123
          Length = 1471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 483 KRLSIGKA 490
>XP_005248122
          Length = 1471

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 483 KRLSIGKA 490
>XP_005248121
          Length = 1543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 555 KRLSIGKA 562
>XP_005248120
          Length = 1549

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 561 KRLSIGKA 568
>XP_006714059
          Length = 1600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248119
          Length = 1601

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248118
          Length = 1602

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248117
          Length = 1626

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248116
          Length = 1627

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248115
          Length = 1629

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>XP_005248114
          Length = 1630

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KQLSIGKS 9
           K+LSIGK+
Sbjct: 642 KRLSIGKA 649
>NP_005076
          Length = 2090

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 205 KQLAVGK 211
>NP_001230280
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           +KQL +GK+
Sbjct: 157 KKQLQLGKT 165
>NP_057202
          Length = 625

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           +KQL +GK+
Sbjct: 157 KKQLQLGKT 165
>NP_004781
          Length = 563

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKQLSIGK 8
           QK+L++GK
Sbjct: 314 QKELTMGK 321
>NP_695008
          Length = 550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKQLSIGK 8
           QK+L++GK
Sbjct: 314 QKELTMGK 321
>NP_695010
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKQLSIGK 8
           QK+L++GK
Sbjct: 314 QKELTMGK 321
>NP_695009
          Length = 506

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKQLSIGK 8
           QK+L++GK
Sbjct: 314 QKELTMGK 321
>NP_006518
          Length = 270

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           QKQ++ GK+
Sbjct: 139 QKQINYGKN 147
>NP_055796
          Length = 1946

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    QKQLSIGKS 9
            QKQL  GK+
Sbjct: 1456 QKQLQSGKA 1464
>XP_006718456
          Length = 913

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 737 KQLTVGK 743
>XP_005272276
          Length = 2036

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 246 KQLAVGK 252
>XP_005272275
          Length = 2083

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 246 KQLAVGK 252
>XP_006717355
          Length = 2120

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 246 KQLAVGK 252
>XP_005272274
          Length = 2121

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 246 KQLAVGK 252
>XP_005272273
          Length = 2132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KQLSIGK 8
           KQL++GK
Sbjct: 246 KQLAVGK 252
>XP_005250069
          Length = 590

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           +KQL +GK+
Sbjct: 157 KKQLQLGKT 165
>XP_005250068
          Length = 615

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           +KQL +GK+
Sbjct: 133 KKQLQLGKT 141
>XP_005250067
          Length = 638

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           +KQL +GK+
Sbjct: 157 KKQLQLGKT 165
>NP_694948
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKQLSIGKS 9
           QK+LS+G S
Sbjct: 135 QKRLSLGYS 143
>NP_001182583
          Length = 502

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKQLSIGKS 9
           QKQ  +GK+
Sbjct: 353 QKQTRVGKA 361
>NP_115916
          Length = 531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKQLSIGKS 9
           QKQ  +GK+
Sbjct: 382 QKQTRVGKA 390
>NP_001182582
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKQLSIGKS 9
           QKQ  +GK+
Sbjct: 362 QKQTRVGKA 370
>NP_001076582
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKQLSIGKS 9
           QKQ  +GK+
Sbjct: 362 QKQTRVGKA 370
>NP_852150
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKQLSIGKS 9
           QKQ  +GK+
Sbjct: 362 QKQTRVGKA 370
>NP_005465
          Length = 1122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 526 QQQLQLGK 533
>NP_001015053
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 527 QQQLQLGK 534
>NP_005885
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KQLSIGKS 9
           KQL  GKS
Sbjct: 283 KQLGCGKS 290
>XP_006721692
          Length = 1081

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 485 QQQLQLGK 492
>XP_005256964
          Length = 1118

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 522 QQQLQLGK 529
>XP_005256963
          Length = 1122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 526 QQQLQLGK 533
>XP_005256962
          Length = 1122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 526 QQQLQLGK 533
>XP_005256961
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKQLSIGK 8
           Q+QL +GK
Sbjct: 527 QQQLQLGK 534
>XP_006720705
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KQLSIGK 8
          KQLS+G+
Sbjct: 6  KQLSLGR 12
>XP_005245659
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KQLSIGKS 9
           KQL  GKS
Sbjct: 283 KQLGCGKS 290
>NP_001123920
          Length = 175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KQLSIGK 8
          KQLS+G+
Sbjct: 16 KQLSLGR 22
>NP_001094874
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  KQLSIGK 8
          KQLS+G+
Sbjct: 6  KQLSLGR 12
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.118    0.275 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 503,410
Number of extensions: 657
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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