Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_997698                                                              17    4312
NP_001597                                                              17    4312
XP_006717059                                                           17    4312
NP_004789                                                              16    7355
NP_710142                                                              16    7355
XP_006712581                                                           16    7355
XP_005246132                                                           16    7355
NP_001073973                                                           16    7355
NP_004612                                                              15    9605
NP_872603                                                              15    9605
NP_061987                                                              15    9605
NP_001273612                                                           15    9605
NP_065122                                                              15    9605
NP_001161048                                                           15    9605
XP_006718961                                                           15    9605
XP_006717209                                                           15    9605
XP_006717208                                                           15    9605
XP_006717207                                                           15    9605
XP_005272093                                                           15    9605
XP_006714294                                                           15    9605
NP_001161049                                                           15    9605
NP_057312                                                              15    9605
NP_001273611                                                           15    9605
>NP_997698
          Length = 2466

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           Q MM+RA+I
Sbjct: 694 QDMMERAII 702
>NP_001597
          Length = 2436

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           Q MM+RA+I
Sbjct: 664 QDMMERAII 672
>XP_006717059
          Length = 2435

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           Q MM+RA+I
Sbjct: 663 QDMMERAII 671
>NP_004789
          Length = 747

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           Q+MMKR V
Sbjct: 623 QQMMKRPV 630
>NP_710142
          Length = 899

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           Q+M+KR V+
Sbjct: 713 QEMLKRMVV 721
>XP_006712581
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKMMKRAV 8
           QK+M+RA+
Sbjct: 598 QKIMQRAI 605
>XP_005246132
          Length = 1047

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKMMKRAV 8
           QK+M+RA+
Sbjct: 598 QKIMQRAI 605
>NP_001073973
          Length = 1052

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKMMKRAV 8
           QK+M+RA+
Sbjct: 598 QKIMQRAI 605
>NP_004612
          Length = 931

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           QK+MKR +
Sbjct: 856 QKIMKRLI 863
>NP_872603
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QKMMKRAV 8
          ++MMKRA+
Sbjct: 65 EEMMKRAL 72
>NP_061987
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           +KMMKR ++
Sbjct: 889 EKMMKRLLM 897
>NP_001273612
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QKMMKRAV 8
          ++MMKRA+
Sbjct: 65 EEMMKRAL 72
>NP_065122
          Length = 862

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           QK+MKR +
Sbjct: 781 QKIMKRLI 788
>NP_001161048
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           QK+MKR +
Sbjct: 665 QKIMKRLI 672
>XP_006718961
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           QK+MKR +
Sbjct: 831 QKIMKRLI 838
>XP_006717209
          Length = 812

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           +KMMKR ++
Sbjct: 801 EKMMKRLLM 809
>XP_006717208
          Length = 838

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           +KMMKR ++
Sbjct: 827 EKMMKRLLM 835
>XP_006717207
          Length = 861

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   QKMMKRAVI 9
           +KMMKR ++
Sbjct: 850 EKMMKRLLM 858
>XP_005272093
          Length = 477

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           QK+MKR +
Sbjct: 396 QKIMKRLI 403
>XP_006714294
          Length = 464

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKMMKRAV 8
           ++MMKRA+
Sbjct: 104 EEMMKRAL 111
>NP_001161049
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKMMKRAV 8
           QK+MKR +
Sbjct: 720 QKIMKRLI 727
>NP_057312
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QKMMKRAV 8
          ++MMKRA+
Sbjct: 65 EEMMKRAL 72
>NP_001273611
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1  QKMMKRAV 8
          ++MMKRA+
Sbjct: 69 EEMMKRAL 76
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.132    0.316 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 269,423
Number of extensions: 364
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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