Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              19     665
NP_444254                                                              19     665
NP_444253                                                              19     665
NP_444255                                                              19     665
XP_005247551                                                           19     665
XP_005247550                                                           19     665
XP_006713712                                                           19     665
XP_005247549                                                           19     665
NP_004620                                                              16    7355
NP_004044                                                              16    7355
NP_001243239                                                           16    7355
NP_001271427                                                           16    7355
NP_055720                                                              16    7355
NP_689559                                                              16    7355
NP_001271428                                                           16    7355
NP_056081                                                              16    7355
NP_001247420                                                           16    7355
XP_003960378                                                           16    7355
XP_006723821                                                           16    7355
XP_006723820                                                           16    7355
XP_006723819                                                           16    7355
XP_006721509                                                           16    7355
XP_006721508                                                           16    7355
XP_006721507                                                           16    7355
XP_003960020                                                           16    7355
XP_005246524                                                           16    7355
XP_006712499                                                           16    7355
XP_006712498                                                           16    7355
XP_003403815                                                           16    7355
NP_963844                                                              16    7355
NP_001277238                                                           15    9605
NP_001894                                                              15    9605
NP_001277236                                                           15    9605
XP_006714599                                                           15    9605
>NP_444256
          Length = 1794

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1626 QKTGNAVRA 1634
>NP_444254
          Length = 1845

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1677 QKTGNAVRA 1685
>NP_444253
          Length = 1914

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1746 QKTGNAVRA 1754
>NP_444255
          Length = 1863

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1695 QKTGNAVRA 1703
>XP_005247551
          Length = 1855

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1687 QKTGNAVRA 1695
>XP_005247550
          Length = 1856

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1688 QKTGNAVRA 1696
>XP_006713712
          Length = 1923

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1756 QKTGNAVRA 1764
>XP_005247549
          Length = 1924

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    QKMGNAVRA 9
            QK GNAVRA
Sbjct: 1756 QKTGNAVRA 1764
>NP_004620
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   QKMGNAVRA 9
           +KMGN  RA
Sbjct: 257 RKMGNPQRA 265
>NP_004044
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  GNAVRA 9
          GNAVRA
Sbjct: 26 GNAVRA 31
>NP_001243239
          Length = 598

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMGNAVR 8
           KMGN+V+
Sbjct: 507 KMGNSVK 513
>NP_001271427
          Length = 573

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMGNAVRA 9
           QK+ NA+RA
Sbjct: 423 QKITNAMRA 431
>NP_055720
          Length = 669

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMGNAVR 8
           KMGN+V+
Sbjct: 507 KMGNSVK 513
>NP_689559
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMGNAVRA 9
           QK+ NA+RA
Sbjct: 419 QKITNAMRA 427
>NP_001271428
          Length = 432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMGNAVRA 9
           QK+ NA+RA
Sbjct: 282 QKITNAMRA 290
>NP_056081
          Length = 581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKMGNAVRA 9
           +KMGN V +
Sbjct: 495 EKMGNTVES 503
>NP_001247420
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKMGNAVRA 9
           +KMGN V +
Sbjct: 511 EKMGNTVES 519
>XP_003960378
          Length = 141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  QKMGNAVR 8
          Q  GNAVR
Sbjct: 73 QSRGNAVR 80
>XP_006723821
          Length = 334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKMGNAVRA 9
           +KMGN V +
Sbjct: 248 EKMGNTVES 256
>XP_006723820
          Length = 334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKMGNAVRA 9
           +KMGN V +
Sbjct: 248 EKMGNTVES 256
>XP_006723819
          Length = 614

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   QKMGNAVRA 9
           +KMGN V +
Sbjct: 528 EKMGNTVES 536
>XP_006721509
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMGNAVRA 9
           QK+ NA+RA
Sbjct: 386 QKITNAMRA 394
>XP_006721508
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMGNAVRA 9
           QK+ NA+RA
Sbjct: 419 QKITNAMRA 427
>XP_006721507
          Length = 569

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKMGNAVRA 9
           QK+ NA+RA
Sbjct: 419 QKITNAMRA 427
>XP_003960020
          Length = 141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  QKMGNAVR 8
          Q  GNAVR
Sbjct: 73 QSRGNAVR 80
>XP_005246524
          Length = 559

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMGNAVR 8
           KMGN+V+
Sbjct: 507 KMGNSVK 513
>XP_006712499
          Length = 604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMGNAVR 8
           KMGN+V+
Sbjct: 507 KMGNSVK 513
>XP_006712498
          Length = 661

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   KMGNAVR 8
           KMGN+V+
Sbjct: 507 KMGNSVK 513
>XP_003403815
          Length = 141

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  QKMGNAVR 8
          Q  GNAVR
Sbjct: 73 QSRGNAVR 80
>NP_963844
          Length = 277

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3 MGNAVRA 9
         MGN +RA
Sbjct: 1 MGNTIRA 7
>NP_001277238
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  KMGNAVRA 9
          K GN VRA
Sbjct: 17 KRGNMVRA 24
>NP_001894
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KMGNAVRA 9
           K GN VRA
Sbjct: 120 KRGNMVRA 127
>NP_001277236
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KMGNAVRA 9
           K GN VRA
Sbjct: 120 KRGNMVRA 127
>XP_006714599
          Length = 906

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   KMGNAVRA 9
           K GN VRA
Sbjct: 120 KRGNMVRA 127
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.120    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 399,907
Number of extensions: 351
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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