Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_078824                                                              18    1482
XP_005247816                                                           18    1482
XP_006713806                                                           18    1482
NP_001159477                                                           17    4312
NP_001159478                                                           17    4312
NP_036424                                                              17    4312
NP_004428                                                              17    4312
NP_976217                                                              17    4312
NP_001269263                                                           17    4312
NP_037444                                                              17    4312
NP_001093646                                                           17    4312
NP_001159479                                                           17    4312
NP_065385                                                              17    4312
NP_976218                                                              17    4312
NP_001269264                                                           17    4312
NP_001245257                                                           17    4312
XP_005259935                                                           17    4312
XP_005259934                                                           17    4312
XP_005259933                                                           17    4312
XP_005259931                                                           17    4312
XP_005259930                                                           17    4312
XP_006722776                                                           17    4312
XP_005245831                                                           17    4312
XP_005245830                                                           17    4312
XP_005245829                                                           17    4312
XP_005245827                                                           17    4312
XP_005245826                                                           17    4312
XP_006710503                                                           17    4312
XP_005245825                                                           17    4312
XP_005245824                                                           17    4312
XP_005245822                                                           17    4312
XP_005245821                                                           17    4312
XP_005245820                                                           17    4312
XP_005245818                                                           17    4312
XP_006710502                                                           17    4312
XP_005245817                                                           17    4312
XP_006710501                                                           17    4312
XP_005245814                                                           17    4312
XP_006710500                                                           17    4312
XP_006710499                                                           17    4312
XP_006710498                                                           17    4312
XP_005245810                                                           17    4312
XP_006710497                                                           17    4312
NP_001231573                                                           16    5631
NP_054762                                                              16    5631
NP_001008723                                                           16    7355
NP_001103373                                                           16    7355
NP_116203                                                              16    7355
XP_005254026                                                           16    7355
XP_005254025                                                           16    7355
NP_065750                                                              15    9605
NP_848018                                                              15    9605
NP_001003396                                                           15    9605
NP_115493                                                              15    9605
XP_005267181                                                           15    9605
XP_006715621                                                           15    9605
XP_006715620                                                           15    9605
XP_005267180                                                           15    9605
XP_005267179                                                           15    9605
XP_006715619                                                           15    9605
XP_005267178                                                           15    9605
XP_005263332                                                           15    9605
XP_006714403                                                           15    9605
XP_005263331                                                           15    9605
XP_005245421                                                           15    9605
XP_006711532                                                           15    9605
XP_005245420                                                           15    9605
NP_777568                                                              15    9605
NP_001003397                                                           15    9605
NP_001003395                                                           15    9605
NP_003278                                                              15    9605
>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKESMKAEA 9
            QKE+MKAEA
Sbjct: 1979 QKEAMKAEA 1987
>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKESMKAEA 9
            QKE+MKAEA
Sbjct: 1979 QKEAMKAEA 1987
>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKESMKAEA 9
            QKE+MKAEA
Sbjct: 1932 QKEAMKAEA 1940
>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKESMKAEA 9
            QKE+MKAEA
Sbjct: 2011 QKEAMKAEA 2019
>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    QKESMKAEA 9
            QKE+MKAEA
Sbjct: 2011 QKEAMKAEA 2019
>NP_078824
          Length = 865

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKES+KAE
Sbjct: 240 QKESLKAE 247
>XP_005247816
          Length = 806

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKES+KAE
Sbjct: 240 QKESLKAE 247
>XP_006713806
          Length = 831

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKES+KAE
Sbjct: 206 QKESLKAE 213
>NP_001159477
          Length = 864

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>NP_001159478
          Length = 720

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>NP_036424
          Length = 1136

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KESMKAEA 9
           K+S+KAEA
Sbjct: 858 KDSLKAEA 865
>NP_004428
          Length = 588

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 379 QKETVKAE 386
>NP_976217
          Length = 641

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 379 QKETVKAE 386
>NP_001269263
          Length = 771

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KESMKAEA 9
           K+S+KAEA
Sbjct: 493 KDSLKAEA 500
>NP_037444
          Length = 719

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 377 QEEAMKAE 384
>NP_001093646
          Length = 717

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 377 QEEAMKAE 384
>NP_001159479
          Length = 601

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 379 QKETVKAE 386
>NP_065385
          Length = 731

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   QKESMKAEA 9
           QKE ++AEA
Sbjct: 383 QKEQLEAEA 391
>NP_976218
          Length = 775

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 553 QKETVKAE 560
>NP_001269264
          Length = 1019

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KESMKAEA 9
           K+S+KAEA
Sbjct: 741 KDSLKAEA 748
>NP_001245257
          Length = 1152

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KESMKAEA 9
           K+S+KAEA
Sbjct: 874 KDSLKAEA 881
>XP_005259935
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 304 QEEAMKAE 311
>XP_005259934
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 304 QEEAMKAE 311
>XP_005259933
          Length = 646

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 304 QEEAMKAE 311
>XP_005259931
          Length = 716

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 377 QEEAMKAE 384
>XP_005259930
          Length = 718

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           Q+E+MKAE
Sbjct: 377 QEEAMKAE 384
>XP_006722776
          Length = 1164

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   KESMKAEA 9
           K+S+KAEA
Sbjct: 886 KDSLKAEA 893
>XP_005245831
          Length = 767

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245830
          Length = 782

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245829
          Length = 787

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245827
          Length = 809

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 587 QKETVKAE 594
>XP_005245826
          Length = 810

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710503
          Length = 817

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 379 QKETVKAE 386
>XP_005245825
          Length = 821

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245824
          Length = 823

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245822
          Length = 829

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245821
          Length = 831

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245820
          Length = 850

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245818
          Length = 864

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710502
          Length = 876

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245817
          Length = 881

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710501
          Length = 972

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245814
          Length = 979

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710500
          Length = 991

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710499
          Length = 993

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710498
          Length = 1012

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_005245810
          Length = 1014

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>XP_006710497
          Length = 1026

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QKE++KAE
Sbjct: 588 QKETVKAE 595
>NP_001231573
          Length = 172

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          QKE+MK E
Sbjct: 88 QKENMKME 95
>NP_054762
          Length = 213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   QKESMKAE 8
           QKE+MK E
Sbjct: 129 QKENMKME 136
>NP_001008723
          Length = 872

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           +KE++KAE
Sbjct: 553 EKETLKAE 560
>NP_001103373
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ESMKAEA 9
          ES+KAEA
Sbjct: 47 ESLKAEA 53
>NP_116203
          Length = 420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ESMKAEA 9
          ES+KAEA
Sbjct: 47 ESLKAEA 53
>XP_005254026
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ESMKAEA 9
          ES+KAEA
Sbjct: 47 ESLKAEA 53
>XP_005254025
          Length = 444

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  ESMKAEA 9
          ES+KAEA
Sbjct: 47 ESLKAEA 53
>NP_065750
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QK+++KAE
Sbjct: 449 QKKALKAE 456
>NP_848018
          Length = 482

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QKESMKAEA 9
          Q+E +KA+A
Sbjct: 63 QEEDLKAQA 71
>NP_001003396
          Length = 144

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
>NP_115493
          Length = 205

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKESMKAE 8
           Q+E +KAE
Sbjct: 133 QREQLKAE 140
>XP_005267181
          Length = 102

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 6  EKEELKAE 13
>XP_006715621
          Length = 163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
>XP_006715620
          Length = 166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
>XP_005267180
          Length = 175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 6  EKEELKAE 13
>XP_005267179
          Length = 175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 6  EKEELKAE 13
>XP_006715619
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
>XP_005267178
          Length = 191

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
>XP_005263332
          Length = 190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKESMKAE 8
           Q+E +KAE
Sbjct: 133 QREQLKAE 140
>XP_006714403
          Length = 190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKESMKAE 8
           Q+E +KAE
Sbjct: 118 QREQLKAE 125
>XP_005263331
          Length = 190

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   QKESMKAE 8
           Q+E +KAE
Sbjct: 118 QREQLKAE 125
>XP_005245421
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QK+++KAE
Sbjct: 449 QKKALKAE 456
>XP_006711532
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QK+++KAE
Sbjct: 449 QKKALKAE 456
>XP_005245420
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   QKESMKAE 8
           QK+++KAE
Sbjct: 455 QKKALKAE 462
>NP_777568
          Length = 306

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  QKESMKAEA 9
          Q+E +KA+A
Sbjct: 63 QEEDLKAQA 71
>NP_001003397
          Length = 131

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
>NP_001003395
          Length = 175

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 6  EKEELKAE 13
>NP_003278
          Length = 204

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  QKESMKAE 8
          +KE +KAE
Sbjct: 35 EKEELKAE 42
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.280   0.0929    0.196 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 410,453
Number of extensions: 893
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)

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