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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     509
XP_006713721                                                           20     509
NP_037379                                                              16    5631
XP_005256232                                                           16    5631
XP_005264323                                                           16    5631
NP_001026                                                              16    7355
NP_037418                                                              16    7355
XP_006711873                                                           16    7355
XP_006711871                                                           16    7355
XP_006711870                                                           16    7355
XP_006711869                                                           16    7355
XP_006711868                                                           16    7355
XP_006711867                                                           16    7355
XP_006711866                                                           16    7355
XP_006711865                                                           16    7355

>NP_006209
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QIMSSAKRP 9
           +IMSSAKRP
Sbjct: 770 RIMSSAKRP 778



>XP_006713721
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   QIMSSAKRP 9
           +IMSSAKRP
Sbjct: 770 RIMSSAKRP 778



>NP_037379
          Length = 458

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IMSSAKRP 9
           I+SSA+RP
Sbjct: 274 IVSSAQRP 281



>XP_005256232
          Length = 2424

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3 MSSAKRP 9
         MSS KRP
Sbjct: 1 MSSVKRP 7



>XP_005264323
          Length = 426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IMSSAKRP 9
           I+SSA+RP
Sbjct: 274 IVSSAQRP 281



>NP_001026
          Length = 4967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4137 EIMGSAKR 4144



>NP_037418
          Length = 890

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SSAKRP 9
          SSAKRP
Sbjct: 18 SSAKRP 23



>XP_006711873
          Length = 4954

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4124 EIMGSAKR 4131



>XP_006711871
          Length = 4906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4076 EIMGSAKR 4083



>XP_006711870
          Length = 4965

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4135 EIMGSAKR 4142



>XP_006711869
          Length = 4973

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4143 EIMGSAKR 4150



>XP_006711868
          Length = 4975

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4145 EIMGSAKR 4152



>XP_006711867
          Length = 4977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4147 EIMGSAKR 4154



>XP_006711866
          Length = 4984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4154 EIMGSAKR 4161



>XP_006711865
          Length = 4985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    QIMSSAKR 8
            +IM SAKR
Sbjct: 4155 EIMGSAKR 4162



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.115    0.292 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 311,767
Number of extensions: 455
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)