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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000312                                                              20     509
NP_060240                                                              16    5631
NP_068586                                                              16    7355
NP_001229757                                                           16    7355
XP_005249324                                                           16    7355
XP_005249323                                                           16    7355
NP_542165                                                              15    9605
NP_073563                                                              15    9605
NP_001098028                                                           15    9605
NP_004631                                                              15    9605
NP_079206                                                              15    9605
XP_005248664                                                           15    9605
XP_005248663                                                           15    9605
XP_006714758                                                           15    9605

>NP_000312
          Length = 928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   QIMMFSMYG 9
           QIMM SMYG
Sbjct: 702 QIMMCSMYG 710



>NP_060240
          Length = 296

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           I+M+S YG
Sbjct: 181 ILMYSYYG 188



>NP_068586
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 178 VLMYSYYG 185



>NP_001229757
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 205 VLMYSYYG 212



>XP_005249324
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 178 VLMYSYYG 185



>XP_005249323
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 178 VLMYSYYG 185



>NP_542165
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   QIMMFS 6
           Q+MMFS
Sbjct: 223 QVMMFS 228



>NP_073563
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 193 VIMYSYYG 200



>NP_001098028
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 182 VVMYSYYG 189



>NP_004631
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   QIMMFS 6
           Q+MMFS
Sbjct: 223 QVMMFS 228



>NP_079206
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 182 VVMYSYYG 189



>XP_005248664
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 169 VVMYSYYG 176



>XP_005248663
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 182 VVMYSYYG 189



>XP_006714758
          Length = 281

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IMMFSMYG 9
           ++M+S YG
Sbjct: 182 VVMYSYYG 189



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.144    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 278,633
Number of extensions: 466
Number of successful extensions: 14
Number of sequences better than 10000.0: 14
Number of HSP's gapped: 14
Number of HSP's successfully gapped: 14
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)